NBD-F;(Synonyms: 4-Fluoro-7-nitrobenzofurazan) 纯度: ge;98.0%
NBD-F (4-Fluoro-7-nitrobenzofurazan) 是一种用于氨基酸分析 (amino acid analysis) 的前荧光试剂。NBD-F 与一级或二级胺反应生成荧光产物,用于分析氨基酸和低分子量胺。
NBD-F Chemical Structure
CAS No. : 29270-56-2
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
10;mM;*;1 mL in DMSO | ¥1540 | In-stock | |
50 mg | ¥1800 | In-stock | |
100 mg | ¥2600 | In-stock | |
200 mg | ; | 询价 | ; |
500 mg | ; | 询价 | ; |
* Please select Quantity before adding items.
NBD-F 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
生物活性 |
NBD-F (4-Fluoro-7-nitrobenzofurazan) is a pro-fluorescent reagent which is developed for amino acid analysis. NBD-F reacts with primary or secondary amines to produce a fluorescent product and used for analysis of amino acids and low molecular weight amines[1]. |
||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
体外研究 (In Vitro) |
As the percentage of the organic phase changes, the retention time of NBD-F remains relatively stable, while the retention times of the derivatization products changes. The pH of the mobile phase affects the separation of the NBD-F and the derivatization products[1]. NBD-F is a fluorescent derivatization reagent that is originally developed for amino acid analysis, and recently applied to the analysis of other amino acid derivatives such as N-methyl-D-aspartic acid and glutathione. The use of NBD-F appears to have several advantages in that the derivatization procedure is simple and its derivatives are highly stable[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
||||||||||||||||
分子量 |
183.10 |
||||||||||||||||
Formula |
C6H2FN3O3 |
||||||||||||||||
CAS 号 |
29270-56-2 |
||||||||||||||||
中文名称 |
4-氟-7-硝基-2,1,3-苯并氧杂噁二唑 |
||||||||||||||||
运输条件 |
Room temperature in continental US; may vary elsewhere. |
||||||||||||||||
储存方式 |
4deg;C, protect from light *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light) |
||||||||||||||||
溶解性数据 |
In Vitro:;
DMSO : 100 mg/mL (546.15 mM; Need ultrasonic) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
|
||||||||||||||||
参考文献 |
|
Kinase Assay [1] |
An accurately weighed quantity (0.0183 g) of NBD-F is transferred into a 1 mL centrifuge tube, dissolved in acetonitrile and made up to volume with the same solvent to produce stock solutions of 0.1 M. The solution is protected from light and stored at -20°C until analyzed. A 100 μL aliquot of mixed amino acids solution or sample supernatant, 175 μL of borate buffer solution, 200 μL of acetonitrile and 25 μL of NBD-F working solution are mixed in a 1.5 mL centrifuge tube. The well-mixed solution is allowed to react at 60°C in the water bath for 7 min, excluding light. NBD-F reacts with amino group and enables amino acids to be detected with UV detection. After cooling to room temperature, 10 μL of the solution is injected into the equilibrated HPLC system[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
---|---|
参考文献 |
|