VPM peptide is a dithiol protease-cleavable peptide cross-linker. VPM peptide can be incorporated into the backbone of the PEG-diacrylate (PEG-DA) macromer to form PEG hydrogel[1][2].
体外研究 (In Vitro)
VPM peptide is rapidly cleaved by matrix metalloproteinase (MMP)-1 and MMP-2 proteases[2]. VPM-crosslinked microgels are degradable by proteases in a concentration-dependent manner[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
1696.95
Formula
C63H109N25O22S4
CAS 号
1428885-83-9
Sequence Shortening
GCRDVPMSMRGGDRCG
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;
H2O
Peptide Solubility and Storage Guidelines:
1.;;Calculate the length of the peptide.
2.;;Calculate the overall charge of the entire peptide according to the following table:
;
Contents
Assign value
Acidic amino acid
Asp (D), Glu (E), and the C-terminal -COOH.
-1
Basic amino acid
Arg (R), Lys (K), His (H), and the N-terminal -NH2
+1
Neutral amino acid
Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)
0
3.;;Recommended solution:
Overall charge of peptide
Details
Negative (lt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH4OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)
1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
[1]. Phelps EA, et, al. Maleimide cross-linked bioactive PEG hydrogel exhibits improved reaction kinetics and cross-linking for cell encapsulation and in situ delivery. Adv Mater. 2012 Jan 3;24(1):64-70, 2
[2]. Foster GA, et, al. Protease-degradable microgels for protein delivery for vascularization. Biomaterials. 2017 Jan;113:170-175.
PEG300 (Polyethylene glycol 300), a neutral polymer of molecular weight 300, is a water-soluble, low immunogenic and biocompatible polymer formed by repeating units of ethylene glycol[1][2].
体外研究 (In Vitro)
The FDA has approved polyethylene glycol (PEG) for use as a vehicle or base in foods, cosmetics and pharmaceuticals, including injectable, topical, rectal and nasal formulations. PEG shows little toxicity, and is eliminated from the body intact by either the kidneys (for PEGs < 30 kDa) or in the faeces (for PEGs > 20 kDa). PEG lacks immunogenicity, and antibodies to PEG are generated in rabbits only if PEG is combined with highly immunogenic proteins[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The final concentration of PEG300 can go up to 50% in the formulations for intravenous and intramuscular injection without any toxic effects. When administered orally, the highest concentration of PEG300 can reach up to 90%[4][5].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
300.00
CAS 号
25322-68-3
中文名称
聚乙二醇
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, sealed storage, away from moisture
溶解性数据
In Vitro:;
DMSO : 100 mg/mL (333.33 mM; Need ultrasonic)
H2O : ≥ 50 mg/mL (166.67 mM)
*“≥” means soluble, but saturation unknown.
参考文献
[1]. J.Billingham, et al. Adsorption of polyamine, polyacrylic acid and polyethylene glycol on montmorillonite: An in situ study using ATR-FTIR. Volume 14, Issue 1, March 1997, Pages 19-34.
[2]. Lee CC, et al. Structural basis of polyethylene glycol recognition by antibody. J Biomed Sci. 2020 Jan 7;27(1):12.
[3]. Harris JM, et al. Effect of pegylation on pharmaceuticals. Nat Rev Drug Discov. 2003 Mar;2(3):214-21.
[5]. Ellen Weisberg, et al. Beneficial effects of combining nilotinib and imatinib in preclinical models of BCR-ABL+ leukemias. Blood. 2007 Mar 1;109(5):2112-20.
PEG400 is a strongly hydrophilic polyethylene glycol used as an excellent solvent for a large number of substances. PEG400 is widely used in a variety of pharmaceutical formulations.
体内研究 (In Vivo)
Treatment of Fischer 344 rats with PEG400 at a constant volume of 1.0, 2.5 or 5.0 mL/kg body weight/day 5 days/wk for 13 week does not result in any mortality attributed to chemical toxicity or changes in haematology or clinical chemistry findings[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The final concentration of PEG300 can go up to 50% in the formulations for intravenous and intramuscular injection without any toxic effects. In PEG400 based solubility-enabling formulations in oral delivery of lipophilic drugs, both the 60% and the 100% PEG-400 formulations allowed full solubilization of the dose throughout the entire gastrointestinal tract-like journey[2][3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Pure form
-20deg;C
3 years
4deg;C
2 years
参考文献
[1]. Hermansky SJ, et al. Effects of polyethylene glycol 400 (PEG 400) following 13 weeks of gavage treatment in Fischer-344 rats. Food Chem Toxicol. 1995 Feb;33(2):139-49.
[3]. Avital Beig, et al. Striking the Optimal Solubility-Permeability Balance in Oral Formulation Development for Lipophilic Drugs: Maximizing Carbamazepine Blood Levels. Mol Pharm. 2017 Jan 3;14(1):319-327.
Animal Administration [1]
Rats[1]
Fischer-344 rats (10/group/sex) are administered polyethylene glycol 400 (PEG400) by gavage at 1.0, 2.5 or 5.0 mL/kg (1. l, 2.8 and 5.6 g/kg, respectively) body weight/day 5 days/wk for 13 wk. Animals in the control group receive water by gavage (5.0 mL/kg body weight/treatment day). An additional 10 rats/sex/group are assigned to the control and high-dose groups for a 6-wk recovery period. Evaluation of potential renal toxicity is identified as a primary objective[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Hermansky SJ, et al. Effects of polyethylene glycol 400 (PEG 400) following 13 weeks of gavage treatment in Fischer-344 rats. Food Chem Toxicol. 1995 Feb;33(2):139-49.
[3]. Avital Beig, et al. Striking the Optimal Solubility-Permeability Balance in Oral Formulation Development for Lipophilic Drugs: Maximizing Carbamazepine Blood Levels. Mol Pharm. 2017 Jan 3;14(1):319-327.
N-(Azido-PEG3)-N-Fluorescein-PEG3-acid Chemical Structure
CAS No. : 2100306-50-9
规格
是否有货
1 mg
询价
* Please select Quantity before adding items.
生物活性
N-(Azido-PEG3)-N-Fluorescein-PEG3-acid is a PEG-based PROTAC linker which contains azide, fluorescein and carboxylic acid moieties.
IC50 Target
PEGs
;
体外研究 (In Vitro)
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
811.85
Formula
C38H45N5O13S
CAS 号
2100306-50-9
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
DBCO-PEG12-TCO cantains a TCO and a DBCO moiety. TCO group can specifically react with terrahydrazine.
体外研究 (In Vitro)
DBCO reacts with azide functionalized compounds or biomolecules without catalyst to form a stable triazole linkage, which is an ideal alternative to copper intolerant applications.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
1028.23
Formula
C54H81N3O16
CAS 号
2055022-06-3
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
[1]. Yin H, et al. Electrochemical immunosensor for N6-methyladenosine detection in human cell lines based on biotin-streptavidin system and silver-SiO2 signal amplification. Biosens Bioelectron. 2017 Apr 15;90:494-500.
18:1 PEG-PE is a polyethyleneglycol/phosphatidyl-ethanolamine conjugate. 18:1 PEG-PE can be used for drug delivery[1].
Formula
C45H87N2O11P
CAS 号
474922-90-2
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. [1]Anatoly N Lukyanov, et al. Increased accumulation of PEG-PE micelles in the area of experimental myocardial infarction in rabbits. J Control Release. 2004 Jan 8;94(1):187-93.
