Autocamtide-2-related inhibitory peptide

发表于2024年7月22日由Whatman官网

Autocamtide-2-related inhibitory peptide;

Autocamtide-2-related inhibitory peptide 是高度特异性的、CaMKII 的有效抑制剂，其IC₅₀ 值为 40 nM。

Autocamtide-2-related inhibitory peptide Chemical Structure

CAS No. : 167114-91-2

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

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Autocamtide-2-related inhibitory peptide 的其他形式现货产品:

Autocamtide-2-related inhibitory peptide TFA

生物活性	Autocamtide-2-related inhibitory peptide is a highly specific and potent inhibitor of CaMKII with an IC₅₀ of 40 nM.
IC₅₀ Target	IC50: 40 nM (CaMKII)^[1].
体外研究 (In Vitro)	It is found that Autocamtide-2-related inhibitory peptide (a novel synthetic peptide ATP) is a potent inhibitor of CaM-kinase II with an IC₅₀ of 40 nM, which is active in the presence or absence of Ca²⁺/calmodulin. It is 50 and 500 times more potent than CaMK-(281-302Ala286) and KN-93,respectively, under the assay condition used^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	1497.74
Formula	C₆₄H₁₁₆N₂₂O₁₉
CAS 号	167114-91-2
Sequence	Lys-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu
Sequence Shortening	KKALRRQEAVDAL
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Ishida A, et al. A novel highly specific and potent inhibitor of calmodulin-dependent protein kinase II. Biochem Biophys Res Commun. 1995 Jul 26;212(3):806-12.

Autocamtide-2-related inhibitory peptide, myristoylated

发表于2024年7月16日由Whatman官网

Autocamtide-2-related inhibitory peptide, myristoylated;

Autocamtide-2-related inhibitory peptide, myristoylated 是Autocamtide-2-related inhibitory peptide 烷基化修饰后的形式。 Autocamtide-2-related inhibitory peptide 是高度特异性的、CaMKII 的有效抑制剂，其IC₅₀ 值为 40 nM。

Autocamtide-2-related inhibitory peptide, myristoylated Chemical Structure

CAS No. : 201422-04-0

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

生物活性	Autocamtide-2-related inhibitory peptide, myristoylated is the myristoylated Autocamtide-2-related inhibitory peptide. Autocamtide-2-related inhibitory peptide is a highly specific and potent inhibitor of CaMKII with an IC₅₀ of 40 nM^[1].
分子量	1708.10
Formula	C₇₈H₁₄₂N₂₂O₂₀
CAS 号	201422-04-0
Sequence	{Lys(Myr)}-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu
Sequence Shortening	{Lys(Myr)}-KALRRQEAVDAL
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Ishida A, et al. A novel highly specific and potent inhibitor of calmodulin-dependent protein kinase II. Biochem Biophys Res Commun. 1995 Jul 26;212(3):806-12.

Calcitonin Gene Related Peptide (CGRP) II, rat

发表于2024年7月16日由Whatman官网

Calcitonin Gene Related Peptide (CGRP) II, rat;

Calcitonin Gene Related Peptide (CGRP) II, rat 是由 37 个氨基酸组成的神经多肽。

Calcitonin Gene Related Peptide (CGRP) II, rat Chemical Structure

CAS No. : 99889-63-1

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

Calcitonin Gene Related Peptide (CGRP) II, rat 的其他形式现货产品:

Calcitonin Gene Related Peptide (CGRP) II, rat TFA

生物活性

Calcitonin Gene Related Peptide (CGRP) II, rat is a neuropeptide with 37 amino acid^[1].

分子量

3805.31

Formula

C₁₆₃H₂₆₇N₅₁O₅₀S₂

CAS 号

99889-63-1

Sequence

Ser-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asp-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2 (Disulfide bridge: Cys2-Cys7)

Sequence Shortening

SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSKAF-NH2 (Disulfide bridge: Cys2-Cys7)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility

In Vitro:;

H₂O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

;	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3.;;Recommended solution:

Overall charge of peptide	Details
Negative (lt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH₄OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

参考文献

[1]. Beglinger C, et al. Calcitonin gene-related peptides I and II and calcitonin: distinct effects on gastric acid secretion in humans. Gastroenterology. 1988 Oct;95(4):958-65.

Autocamtide-2-related inhibitory peptide, myristoylated TFA

发表于2024年7月6日由Whatman官网

Autocamtide-2-related inhibitory peptide, myristoylated TFA;

Autocamtide-2-related inhibitory peptide, myristoylated TFA 是Autocamtide-2-related inhibitory peptide 烷基化修饰后的形式。 Autocamtide-2-related inhibitory peptide 是高度特异性的、CaMKII 的有效抑制剂，其IC₅₀ 值为 40 nM。

Autocamtide-2-related inhibitory peptide, myristoylated TFA Chemical Structure

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

生物活性	Autocamtide-2-related inhibitory peptide, myristoylated TFA is the myristoylated Autocamtide-2-related inhibitory peptide. Autocamtide-2-related inhibitory peptide is a highly specific and potent inhibitor of CaMKII with an IC₅₀ of 40 nM^[1].
分子量	1822.12
Formula	C₇₈H₁₄₂N₂₂O₂₀.C₂HF₃O₂
Sequence	{Lys(Myr)}-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu
Sequence Shortening	{Lys(Myr)}-KALRRQEAVDAL
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Ishida A, et al. A novel highly specific and potent inhibitor of calmodulin-dependent protein kinase II. Biochem Biophys Res Commun. 1995 Jul 26;212(3):806-12.

Eledoisin Related Peptide(Synonyms: Eledoisin-Related Peptide Eledoisin RP)

发表于2024年7月1日由Whatman官网

Eledoisin Related Peptide;(Synonyms: Eledoisin-Related Peptide; Eledoisin RP)

Eledoisin Related Peptide是物质P的类似物，刺激神经元并触发行为反应。它也是 tachykinin receptor 的配体。

Eledoisin Related Peptideamp;;(Synonyms: Eledoisin-Related Peptide; Eledoisin RP)

Eledoisin Related Peptide Chemical Structure

CAS No. : 2990-43-4

规格	价格	是否有货
1 mg	￥770	询问价格货期
5 mg	￥3100	询问价格货期
10 mg	￥4900	询问价格货期

* Please select Quantity before adding items.

生物活性

Eledoisin Related Peptide is a Substance P analog that excites neurons and triggers behavioral responses. Eledoisin Related Peptide is also a tachykinin receptor ligand.

IC₅₀ Target

Tachykinin receptor^[1]

体内研究
(In Vivo)

Eledoisin Related Peptide shares with Substance P (SP) a common N-terminal amino acid sequence and has been shown by to have SP-like activity in the periphery (gut and salivary glands) and the CNS. Eledoisin-related peptide seems to be roughly equipotent with Substance P at identical ejection currents on the single-cell activity of neurons in this nucleus^[2]. Both glutamate and substance P (and its analogue, eledoisin-related peptide) have excitatory effects on the activity of respiratory neurons and reflex interneurons^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

706.94

Formula

C₃₄H₅₈N₈O₆S

CAS 号

2990-43-4

Sequence

Lys-Phe-Ile-Gly-Leu-Met-NH2

Sequence Shortening

KFIGLM-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility

In Vitro:;

H₂O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

;	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3.;;Recommended solution:

Overall charge of peptide	Details
Negative (lt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH₄OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

参考文献

[1]. Severini C, et al. The tachykinin peptide family. Pharmacol Rev. 2002 Jun;54(2):285-322.

[2]. Guyenet PG, et al. Excitation of neurons in the nucleus locus coeruleus by substance P and related peptides. Brain Res. 1977 Nov 4;136(1):178-84.

[3]. Henry JL, et al. Effects of glutamate, substance P and eledoisin-related peptide on solitary tract neurones involved in respiration and respiratory reflexes. Neuroscience. 1985 Mar;14(3):863-73.

Animal Administration ^[2]	Rats^[2] Twenty-two male albino rats (230-260 g) are anesthetized with chloral hydrate (400 mg/kg, i.p.) and prepared for recording. Briefly, a singlebarrel recording pipette (tip 1 μm) is glued alongside a conventional five-barrel micropipette (tip 15-25/μm) then filled with 2 M NaCI saturated with Fast Green (impedance 4-7 M). The distance between the tip of the recording electrode and that of the five-barrel micropipette is 15-25/zm. Fast Green is ejected at the end of the experiment to identify the recording site. One side barrel of the five-barrel micropipette is loaded with 4 M NaCI for automatic current balancing and the others with three of the following solutions: L-epinephrine bitartrate (0. l M, pH 4.0), L-norepinephrine bitartrate (0.1 M, pH 4.0), Substance P (2.75 mM), physalaemin (2.6 mM), substance P 4-11 octapeptide (3.1 mM), eledoisin-related peptide (20 mM), neurotensin, bradykinin triacetate (15 mM), met-enkephalin (6.5 mM), TRH (48 mM). Spontaneously active cells are recorded in the locus coeruleus or in the nearby mesencephalic nucleus of the fifth nerve whose cells are easily identified by their increased activity upon manipulation of the jaw^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Severini C, et al. The tachykinin peptide family. Pharmacol Rev. 2002 Jun;54(2):285-322. [2]. Guyenet PG, et al. Excitation of neurons in the nucleus locus coeruleus by substance P and related peptides. Brain Res. 1977 Nov 4;136(1):178-84. [3]. Henry JL, et al. Effects of glutamate, substance P and eledoisin-related peptide on solitary tract neurones involved in respiration and respiratory reflexes. Neuroscience. 1985 Mar;14(3):863-73.

Animal Administration
^[2]

Rats^[2]

Twenty-two male albino rats (230-260 g) are anesthetized with chloral hydrate (400 mg/kg, i.p.) and prepared for recording. Briefly, a singlebarrel recording pipette (tip 1 μm) is glued alongside a conventional five-barrel micropipette (tip 15-25/μm) then filled with 2 M NaCI saturated with Fast Green (impedance 4-7 M). The distance between the tip of the recording electrode and that of the five-barrel micropipette is 15-25/zm. Fast Green is ejected at the end of the experiment to identify the recording site. One side barrel of the five-barrel micropipette is loaded with 4 M NaCI for automatic current balancing and the others with three of the following solutions: L-epinephrine bitartrate (0. l M, pH 4.0), L-norepinephrine bitartrate (0.1 M, pH 4.0), Substance P (2.75 mM), physalaemin (2.6 mM), substance P 4-11 octapeptide (3.1 mM), eledoisin-related peptide (20 mM), neurotensin, bradykinin triacetate (15 mM), met-enkephalin (6.5 mM), TRH (48 mM). Spontaneously active cells are recorded in the locus coeruleus or in the nearby mesencephalic nucleus of the fifth nerve whose cells are easily identified by their increased activity upon manipulation of the jaw^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Severini C, et al. The tachykinin peptide family. Pharmacol Rev. 2002 Jun;54(2):285-322.

[2]. Guyenet PG, et al. Excitation of neurons in the nucleus locus coeruleus by substance P and related peptides. Brain Res. 1977 Nov 4;136(1):178-84.

[3]. Henry JL, et al. Effects of glutamate, substance P and eledoisin-related peptide on solitary tract neurones involved in respiration and respiratory reflexes. Neuroscience. 1985 Mar;14(3):863-73.

Calcitonin Gene Related Peptide (CGRP) II, rat TFA

发表于2024年4月11日由Whatman官网

Calcitonin Gene Related Peptide (CGRP) II, rat TFA; 纯度: 98.25%

Calcitonin Gene Related Peptide (CGRP) II, rat (TFA) 是由 37 个氨基酸组成的神经多肽。

Calcitonin Gene Related Peptide (CGRP) II, rat TFAamp;;

Calcitonin Gene Related Peptide (CGRP) II, rat TFA Chemical Structure

规格	价格	是否有货	数量
5 mg	￥950	In-stock
10 mg	￥1600	In-stock
50 mg	;	询价	;
100 mg	;	询价	;

* Please select Quantity before adding items.

Calcitonin Gene Related Peptide (CGRP) II, rat TFA 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus
Peptide Library

生物活性

Calcitonin Gene Related Peptide (CGRP) II, rat (TFA) is a neuropeptide with 37 amino acid^[1].

分子量

3919.33

Formula

C₁₆₅H₂₆₈F₃N₅₁O₅₂S₂

Sequence

Ser-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asp-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2 (Disulfide bridge: Cys2-Cys7)

Sequence Shortening

SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSKAF-NH2 (Disulfide bridge: Cys2-Cys7)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80deg;C	2 years
	-20deg;C	1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

Solvent Solubility

In Vitro:;

H₂O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

;	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3.;;Recommended solution:

Overall charge of peptide	Details
Negative (lt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH₄OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

参考文献

[1]. Beglinger C, et al. Calcitonin gene-related peptides I and II and calcitonin: distinct effects on gastric acid secretion in humans. Gastroenterology. 1988 Oct;95(4):958-65.

Autocamtide-2-related inhibitory peptide TFA

发表于2024年4月9日由Whatman官网

Autocamtide-2-related inhibitory peptide TFA 纯度: 95.85%

Autocamtide-2-related inhibitory peptide (TFA) 是高度特异性的、CaMKII 的有效抑制剂，其 IC₅₀ 值为 40 nM。

Autocamtide-2-related inhibitory peptide TFA Chemical Structure

规格	价格	是否有货
1 mg	￥2200	In-stock
5 mg	￥8900	In-stock
10 mg		询价
50 mg		询价

* Please select Quantity before adding items.

Autocamtide-2-related inhibitory peptide TFA 相关产品

•相关化合物库:

Bioactive Compound Library Plus
Peptide Library

生物活性

Autocamtide-2-related inhibitory peptide (TFA) is a highly specific and potent inhibitor of CaMKII with an IC₅₀ of 40 nM.

IC₅₀ & Target

IC50: 40 nM (CaMKII)^[1].

体外研究
(In Vitro)

It is found that Autocamtide-2-related inhibitory peptide (TFA) is a potent inhibitor of CaM-kinase II with an IC₅₀ of 40 nM, which is active in the presence or absence of Ca²⁺/calmodulin. It is 50 and 500 times more potent than CaMK-(281-302Ala286) and KN-93, respectively, under the assay condition used^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1611.76

Formula

C₆₆H₁₂₆N₂₂O₂₁F₃

Sequence

Lys-Lys-Ala-Lru-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu

Sequence Shortening

KKALRRQEAVDAL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

H₂O : 110 mg/mL (68.25 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.6204 mL	3.1022 mL	6.2044 mL
5 mM	0.1241 mL	0.6204 mL	1.2409 mL
10 mM	0.0620 mL	0.3102 mL	0.6204 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

参考文献

[1]. Ishida A, et al. A novel highly specific and potent inhibitor of calmodulin-dependent protein kinase II. Biochem Biophys Res Commun. 1995 Jul 26;212(3):806-12.

[1]. Ishida A, et al. A novel highly specific and potent inhibitor of calmodulin-dependent protein kinase II. Biochem Biophys Res Commun. 1995 Jul 26;212(3):806-12.

Anti-SOD1（ALS-related mutants）Cocktail，Human，Rat-Mono （MS785/MS27）全面检测ALS相关SOD1突变体的混合型抗体

发表于2024年3月20日由Whatman官网

Anti-SOD1（ALS-related mutants）Cocktail，Human，Rat-Mono （MS785/MS27）
全面检测ALS相关SOD1突变体的混合型抗体

产品特性
相关资料
Q&A
参考文献

全面检测ALS相关SOD1突变体的混合型抗体

Anti-SOD1（ALS-related mutants）Cocktail，Human，Rat-Mono（MS785/MS27）

本产品为两种针对SOD1突变体的抗体混合物。SOD1突变体被认为是导致神经性疾病肌萎缩性侧索硬化(ALS)的原因之一，本产品可特异性识别SOD1突变体的共通二级结构，并且通过免疫沉淀后以Western blot或免疫染色等方法可以检测出上百种SOD1突变体。

另外，本混合型抗体也适用于缺锌性内质网应激相关的SOD1研究。详细请浏览“MEMO——锌缺乏和SOD1立体结构变化”。

※ 本产品仅供研究。研究以外不可使用。

※ 本产品基于东京大学药学系研究科细胞情报学教室的研究成果产品化而成。

Anti-SOD1（ALS-related mutants）Cocktail，Human，Rat-Mono （MS785/MS27）全面检测ALS相关SOD1突变体的混合型抗体

免疫沉淀后突变体SOD1的检测实例

在HEK293细胞中表达野生型以及不同突变型SOD1，裂解细胞后添加各抗体（MS785：5 μg/mL, MS27：2 μg/mL, MS785/MS27混合：1 μg/mL）并反应10小时，然后使用Protein G 磁珠进行免疫沉淀。结果显示，MS785和MS27在经过免疫沉淀反应后，未识别出野生型（WT）但与突变体特异性结合。在抗原位点出现突变时，MS785和MS27无法单独识别，但是补充MS785/MS27混合体后，即可检测出来。

◆肌萎缩性侧索硬化（ALS）与SOD1

肌萎缩性侧索硬化(ALS；Amyotrophic Lateral Sclerosis)是一种由于运动神经的细胞死亡导致全身骨骼肌无力、主要症状为肌肉萎缩的神经退行性疾病。90%的ALS为非遗传性发病，10%左右为遗传性发病，并且已经鉴定出了约20种致病基因。其中因氧化还原酶SOD1（Cu/Zn Superoxide Dismutase）的突变导致发病的案例最多，而表达SOD1突变体的转基因小鼠也作为ALS的动物模型使用。借助各种研究手段我们有望阐明由SOD1突变导致的ALS发病机理。

SOD1是一种在活性中心带有铜离子与锌离子的金属酶，主要负责分解细胞内产生的活性氧。虽然SOD1是一种相对较小的蛋白，只有154个氨基酸（人）、分子量仅为24 kDa，但是却有着上百种突变体（图1）。这些突变体基本不会影响分解天然活性氧的酶活性。因此，研究认为，ALS的发病中存在着酶活性以外的其他因素，而伴随突变的毒性获得（toxic gain of function）被视为极有可能的因素。

原本，野生型SOD1形成二聚体，但有关SOD1突变体，东京大学一条秀宪教授等人已澄清了的以下要点：

●　上百种各形各式的SOD1突变体采用了与野生型SOD1不同的共通二级结构（图2）。

●　通过与内质网中的蛋白降解途径（ERAD）的构成蛋白Derlin-1相结合获得细胞毒性。

并且，以SOD1突变体与Derilin-1结合位点为基础，成功开发出能够特异性识别突变体立体结构的大鼠单克隆抗体，克隆MS785和MS27。这些抗体作为阐明SOD1突变引发的ALS发病机制的优秀工具备受期待。

Anti-SOD1（ALS-related mutants）Cocktail，Human，Rat-Mono （MS785/MS27）全面检测ALS相关SOD1突变体的混合型抗体

图1 报告中SOD1突变位点的案例

Anti-SOD1（ALS-related mutants）Cocktail，Human，Rat-Mono （MS785/MS27）全面检测ALS相关SOD1突变体的混合型抗体

图2 SOD1野生型与突变体的结构区别以及抗体识别位点

◆特点

●　混合了抗SOD1大鼠单克隆抗体克隆MS785和克隆MS27的混合型抗体。

※　亦可分别购入克隆MS785、MS27。

●　克隆MS785和MS27识别SOD1突变体共通的单体二级结构，且拥有在免疫沉淀后检测出上百种SOD1突变体的实绩。

●　可以通过免疫细胞染色法特异性检测SOD1突变体。

●　在缺锌条件下可以检测作为内质网应激的原因之一的野生型SOD1二级结构变化。适用于内质网应激研究和SOD1基础研究。详细请浏览“MEMO——锌缺乏和SOD1二级结构改变”。

◆抗体信息

克隆名	MS785	MS27
抗体种类	大鼠单克隆抗体
同种型	IgG2b/κ	IgG2a/κ
交叉性	人
性状	Protein G纯化（0.5 mg/mL），storage buffer：50％甘油／PBS

■　关于克隆MS785与MS27的区别

●　MS785是以人SOD1的6~16氨基酸序列，MS27是以人SOD1的30~40氨基酸序列分别作为抗原制备的单抗。

●　均可广泛地检测突变体，但无法检测到某些每个表位均有突变的特定突变体。

●　由于MS785和MS27有互补性，所以混合型抗体可以补全抗原位点突变体的检测。

◆应用

●　免疫沉淀，免疫染色：突变体检测

●　ELISA：突变体检测（详细请参考原著文献2。）

●　Western blot：同时检测突变体与野生型

注意：在变性状态下，不仅会检测到SOD1突变体，也会检测到野生型SOD1。因此，实验过程中必须防止蛋白变性，请勿使用SDS等变性剂。

◆原著文献

1.　Fujisawa, et al., Ann. Neurol., 72, 739～749 (2012).

1.　"A novel monoclonal antibody reveals a conformational alteration shared by amyotrophic lateral

1.　sclerosis-linked SOD1 mutants."

2.　Fujisawa, et al., Neurobiol. Dis., 82, 478～486 (2015).

2.　"A systematic immunoprecipitation approach reinforces the concept of common conformational

1.　alterations in amyotrophic lateral sclerosis-linked SOD1 mutants."

◆通过免疫染色对SOD1突变体的检测实例

在HEK293细胞表达野生型以及G93A突变体SOD1（FLAG融合蛋白），分别使用MS785、MS27以及MS785/MS27混合型抗体（红）和抗FLAG抗体（绿）进行免疫染色（工作浓度：1 μg/mL）。可以确认，野生型以及G93A突变体均倍倍抗FLAG抗体检测到信号，但是MS785、MS27以及MS785/27混合型抗体均不与野生型反应，仅与突变体反应。

Anti-SOD1（ALS-related mutants）Cocktail，Human，Rat-Mono （MS785/MS27）全面检测ALS相关SOD1突变体的混合型抗体

◆缺锌状态下野生型SOD1的检测实例

本抗体可检测出缺锌时野生型SOD1的动态结构变化。不仅ALS研究，也适用于SOD1的基础研究。

●　本产品有在血清缺乏条件以及锌螯合剂TPEN处理下检测出内源性野生型SOD1的检测实例。

●　MS785、MS27以及混合物都可用于检测。

锌缺乏和SOD1立体构造改变

OD1是拥有锌离子的金属蛋白。东京大学的一条教授等人明确，在缺锌状态下野生型SOD1会释放锌离子，改变二级结构，并采取SOD1突变体相似的单体结构。这种结构变化，令原本不结合的内质网相关性降解（ERAD）蛋白Derlin-1获得结合能力，诱导了ER 应激（内质网应激）。即使是野生型SOD1，本产品也能识别出缺锌状态下的立体结构变化，检测出缺乏锌的SOD1。因此适用于SOD1基础研究以及ER应激研究。

Anti-SOD1（ALS-related mutants）Cocktail，Human，Rat-Mono （MS785/MS27）全面检测ALS相关SOD1突变体的混合型抗体

参考文献：

1. Fujisawa, et al., Ann. Neurol., 72, 739～749 (2012).

1. "A novel monoclonal antibody reveals a conformational alteration shared by amyotrophic lateral sclerosis-linked SOD1 mutants."

2. Homma, et al., Molecular Cell, 52, 75～86 (2013).

1. "SOD1 as a Molecular Switch for Initiating the Homeostatic ER Stress Response under Zinc Deficiency."

3. Fujisawa, et al., Neurobiol. Dis., 82, 478～486 (2015).

1. "A systematic immunoprecipitation approach reinforces the concept of common conformational alterations in amyotrophic lateral sclerosis-linked SOD1 mutants."

Anti-SOD1（ALS-related mutants）Cocktail，Human，Rat-Mono （MS785/MS27）全面检测ALS相关SOD1突变体的混合型抗体

将HEK293细胞用10 μM锌螯合剂TPEN处理8小时。细胞裂解后，分别使用MS785、MS27以及MS785/MS27混合抗体进行免疫沉淀。由于锌离子的缺失，内源性野生型SOD1与SOD1突变体同样被MS785、MS27检测出来。

◆储存温度

20°C

※ 本页面产品仅供研究用，研究以外不可使用。

产品列表

产品编号	产品名称	产品规格	产品等级	备注
FDV-0021A	Anti-SOD1(ALS-related mutants)Cocktail, Human, Rat-Mono(MS785/MS27) Anti-SOD1（ALS相关突变体）混合型，人，大鼠单克隆（MS785/MS27）	100 μg
FDV-0021B	Anti-SOD1(ALS-related mutants), Human, Rat-Mono(MS785) Anti-SOD1（ALS相关突变体）,人，大鼠单克隆（MS785）	100 μg
FDV-0021C	Anti-SOD1(ALS-related mutants), Human, Rat-Mono(MS27) Anti-SOD1（ALS相关突变体）,人，大鼠单克隆（MS27）	100 μg

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
YIF-LF-P0141	–	0.2 mg	–		–	–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
YIF-LF-P0326	–	0.5 mg	–		–	–

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Rac1 (Ras-related C3 Botulinum Toxin Substrate 1) (human) (rec.)

Rac1（Ras相关C3肉毒杆菌毒素底物1）（人）（rec.）

Sumo1 (Small Ubiquitin-related Modifier) (human) (rec.)

Sumo1（小泛素相关调节剂）（人）（rec.）

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