Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.
IC50 Target
Caspase
;
体外研究 (In Vitro)
Biotin-VAD-FMK is a synthetic peptide designed as a methyl ester to facilitate cell permeability.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
To examine whether caspase-2 is activated in the absence of proteolytic cleavage, an in vivo affinity labeling approach is used, using the biotinylated caspase inhibitor biotin-VAD-fmk that detects only active caspases[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
672.81
Formula
C30H49FN6O8S
CAS 号
1135688-15-1
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Li J, et al. Nitric oxide suppresses apoptosis via interrupting caspase activation and mitochondrial dysfunction in cultured hepatocytes. J Biol Chem. 1999 Jun 11;274(24):17325-33.
[2]. Samraj AK, et al. Loss of caspase-9 reveals its essential role for caspase-2 activation and mitochondrial membranedepolarization. Mol Biol Cell. 2007 Jan;18(1):84-93.