荧光染料Biotin-probe 1

荧光染料Dye Reagents Biotin-probe 1;

Biotin-probe 1 是一种非放射性标记的探针。采用生物素标记的探针,可应用于原位杂交技术。

Biotin-probe 1

Biotin-probe 1 Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Biotin-probe 1 is a non-radiolabeled probe. Biotin-labeled probes can be applied to in situ hybridization[1].

分子量

714.92

Formula

C32H58N8O8S

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Bloch B.Biotinylated probes for in situ hybridization histochemistry: use for mRNA detection. J Histochem Cytochem. 1993 Dec;41(12):1751-4.

Biotin NHS(Synonyms: Biotin-NHS Biotin N-hydroxysuccinimide ester NHS-Biotin)

Biotin NHS;(Synonyms: Biotin-NHS; Biotin N-hydroxysuccinimide ester; NHS-Biotin) 纯度: ge;98.0%

Biotin NHS是用于制备生物素化表面或多肽的氨基反应性生物素试剂。

Biotin NHSamp;;(Synonyms: Biotin-NHS;  Biotin N-hydroxysuccinimide ester;  NHS-Biotin)

Biotin NHS Chemical Structure

CAS No. : 35013-72-0

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
100 mg ¥600 In-stock
200 mg ¥960 In-stock
500 mg ; 询价 ;
1 g ; 询价 ;

* Please select Quantity before adding items.

Biotin NHS 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Calculations:
For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.
1.Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess:
mL protein × (mg protein/mL protein) × (mmoL protein/mg protein) × (20 mmoL protein/mmoL protein) = mmoL Biotin.
* 20 = Recommended molar fold excess of biotin for 2 mg/ml protein sample
2. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction:
mmoL Biotin × (1000000 μL/L) × (L/ 10 mmoL) =μL Biotin
Biotin Labeling Reaction:
1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations.
2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO.
3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution.
4. Incubate reaction on ice for two hours or at room temperature for 30 minutes.
5. To tests the labeled protein by ELISA or Western blot.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

341.38

Formula

C14H19N3O5S

CAS 号

35013-72-0

中文名称

生物素N-羟基琥珀酰亚胺酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*该产品在溶液状态不稳定,建议您现用现配,即刻使用。

溶解性数据
In Vitro:;

DMSO : 62.5 mg/mL (183.08 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9293 mL 14.6464 mL 29.2929 mL
5 mM 0.5859 mL 2.9293 mL 5.8586 mL
10 mM 0.2929 mL 1.4646 mL 2.9293 mL

*

请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液;该产品在溶液状态不稳定,建议您现用现配,即刻使用

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (6.09 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.09 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.09 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.09 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.09 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.09 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Wojchowski DM et al. Hybridoma production by simplified avidin-mediated electrofusion. J Immunol Methods. 1986 Jun 24;90(2):173-7.

    [2]. Ngo TT et al. Biotinyl-glucose-6-phosphate dehydrogenase preparation, kinetics, and modulation by avidin. Appl Biochem Biotechnol. 1982 Nov;7(6):443-54.

Biotin-azide(Synonyms: N-(3-Azidopropyl)biotinamide)

生化分析试剂 Biochemical Assay Reagents
Biotin-azide;(Synonyms: N-(3-Azidopropyl)biotinamide) 纯度: 99.64%

Biotin-azide (N-(3-Azidopropyl)biotinamide) 是具有末端叠氮基团的生物素的一种形式。Biotin-azide 用于通过 Click Chemistry 制备各种生物素化的缀合物。

Biotin-azide(Synonyms: N-(3-Azidopropyl)biotinamide)

Biotin-azide Chemical Structure

CAS No. : 908007-17-0

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1500 In-stock
5 mg ¥1360 In-stock
10 mg ¥1800 In-stock
25 mg ¥3200 In-stock
50 mg ¥5400 In-stock
100 mg ¥8500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Biotin-azide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Biotin-azide (N-(3-Azidopropyl)biotinamide) is a form of biotin with a terminal azide group. Biotin-azide can be used to prepare various biotinylated conjugates via Click Chemistry[1][2].

分子量

326.42

Formula

C13H22N6O2S

CAS 号

908007-17-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 250 mg/mL (765.88 mM; Need ultrasonic)

H2O : 4 mg/mL (12.25 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.0635 mL 15.3177 mL 30.6354 mL
5 mM 0.6127 mL 3.0635 mL 6.1271 mL
10 mM 0.3064 mL 1.5318 mL 3.0635 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.37 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.37 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.37 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Bruckman MA, et al. Tobacco mosaic virus-based protein nanoparticles and nanorods for chemotherapy delivery targeting breast cancer. J Control Release. 2016;231:103‐113.

    [2]. Kim HY, et al. An azido-biotin reagent for use in the isolation of protein adducts of lipid-derived electrophiles by streptavidin catch and photorelease. Mol Cell Proteomics. 2009;8(9):2080‐2089.

ATP-polyamine-biotin(Synonyms: ATP多胺生物素)

生化分析试剂 Biochemical Assay Reagents
ATP-polyamine-biotin;(Synonyms: ATP多胺生物素) 纯度: 99.95%

ATP-polyamine-biotin,是第一种可透过细胞的 ATP 类似物,是一种有效的激酶共底物。ATP-polyamine-biotin 可促进活细胞中激酶底物的生物素标记。

ATP-polyamine-biotin(Synonyms: ATP多胺生物素)

ATP-polyamine-biotin Chemical Structure

CAS No. : 1800401-93-7

规格 价格 是否有货 数量
1 mg ¥4500 In-stock
2 mg ¥8000 In-stock
5 mg ¥14000 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

ATP-polyamine-biotin 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells[1].

体外研究
(In Vitro)

ATP-polyamine-biotin (APB) is incubated with PKA kinase and full-length protein substrate, myelin basic protein (MBP). Biotinylation was observed only in presence of kinase. In addition, MBP biotinylation was lost in the absence of ATP-polyamine-biotin, in presence of the kinase inhibitor staursporine or upon incubation with acid due to cleavage of phosphoramidate bond. Biotinylated kemptide product is observed only in the presence of APB cosubstrate[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

945.85

Formula

C32H58N11O14P3S

CAS 号

1800401-93-7

中文名称

ATP多胺生物素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

H2O : 6 mg/mL (6.34 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0573 mL 5.2863 mL 10.5725 mL
5 mM 0.2115 mL 1.0573 mL 2.1145 mL
10 mM

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Fouda AE, et al. A Cell-Permeable ATP Analogue for Kinase-Catalyzed Biotinylation. Angew Chem Int Ed Engl. 2015 Aug 10;54(33):9618-21.

荧光染料L-Biotin-NH-5MP

荧光染料Dye Reagents L-Biotin-NH-5MP;

L-Biotin-NH-5MP 是一种生物素偶联的 5-亚甲基吡咯酮 (5MP)。 5-亚甲基吡咯酮 (5MPs) 是一种硫醇特异性的、可逆的生物偶联试剂,可用于半胱氨酸特异性蛋白质修饰,常用于蛋白生物偶联。

L-Biotin-NH-5MP

L-Biotin-NH-5MP Chemical Structure

CAS No. : 2100288-18-2

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

L-Biotin-NH-5MP is a biotin-conjugated 5-Methylene pyrrolone (5MP). 5-Methylene pyrrolones (5MPs) are thiol-specific, reversible bioconjugation reagents for cysteine-specific protein modification, that are commonly used for protein bioconjugation[1].

分子量

336.41

Formula

C15H20N4O3S

CAS 号

2100288-18-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Yingqian Zhang, et al. Thiol Specific and Tracelessly Removable Bioconjugation via Michael Addition to 5-Methylene Pyrrolones. J Am Chem Soc. 2017 May 3;139(17):6146-6151.

Biotin-DADOO(Synonyms: Biotinyl-3,6-dioxaoctanediamine; EZ-Link Amine-PEO2-Biotin)

生化分析试剂 Biochemical Assay Reagents
Biotin-DADOO;(Synonyms: Biotinyl-3,6-dioxaoctanediamine; EZ-Link Amine-PEO2-Biotin) 纯度: ge;98.0%

Biotin-DADOO 是一个生物素酰化试剂,可用于合成生物素-雌二醇共轭化合物,从而通过酶免疫测定法测定血浆雌二醇的浓度。

Biotin-DADOO(Synonyms: Biotinyl-3,6-dioxaoctanediamine;  EZ-Link Amine-PEO2-Biotin)

Biotin-DADOO Chemical Structure

CAS No. : 138529-46-1

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ¥1320 In-stock
10 mg ¥1200 In-stock
50 mg ¥3600 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

Biotin-DADOO 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Biotin-DADOO is a biotinylation reagent, which can be used to synthesize a biotin-estradiol conjugate (i.e., biotin-DADOO-estradiol) to develop a direct, broad range enzyme immunoassay to measure plasma estradiol concentrations.

分子量

374.50

Formula

C16H30N4O4S

CAS 号

138529-46-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (267.02 mM; Need ultrasonic)

H2O : ≥ 100 mg/mL (267.02 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.6702 mL 13.3511 mL 26.7023 mL
5 mM 0.5340 mL 2.6702 mL 5.3405 mL
10 mM 0.2670 mL 1.3351 mL 2.6702 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.68 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.68 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.68 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (6.68 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. You L, et al. Scutellarin inhibits Hela cell growth and glycolysis by inhibiting the activity of pyruvate kinase M2. Bioorg Med Chem Lett. 2017 Dec 15;27(24):5404-5408.

荧光染料Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide

荧光染料Dye Reagents Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide; 纯度: 95.02%

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide 是一种可点击、酸裂解的 Biotin-picolyl azide。 Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide 是细胞表面糖蛋白的富集手柄,可用于蛋白质标记。

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azideamp;;

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide Chemical Structure

CAS No. : 2599839-59-3

规格 价格 是否有货 数量
5 mg ¥3900 In-stock
10 mg ¥6200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide is a clickable, acid-cleavable biotin-picolyl azide. Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide is an enrichment handle of cell surface glycoproteins for protein labeling[1].

分子量

978.24

Formula

C47H67N9O10SSi

CAS 号

2599839-59-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (stored under nitrogen)

参考文献
  • [1]. Benjamin Schumann, et al. Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells. Mol Cell. 2020 Jun 4;78(5):824-834.e15.

Biotin Hydrazide(Synonyms: 生物素酰肼)

生化分析试剂 Biochemical Assay Reagents
Biotin Hydrazide;(Synonyms: 生物素酰肼) 纯度: ge;98.0%

Biotin Hydrazide 是羰基反应性生物素化试剂,是一种羰基探针。

Biotin Hydrazide(Synonyms: 生物素酰肼)

Biotin Hydrazide Chemical Structure

CAS No. : 66640-86-6

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥605 In-stock
100 mg ¥550 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Biotin Hydrazide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Biotin Hydrazide is a carbonyl-reactive biotinylation reagent, which is a carbonyl probe.

体外研究
(In Vitro)

Biotin Hydrazide is a biotinylation reagent used to biotinylate glycoproteins and glycolipids[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

258.34

Formula

C10H18N4O2S

CAS 号

66640-86-6

中文名称

生物素酰肼

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 6.6 mg/mL (25.55 mM; Need ultrasonic and warming)

H2O : 2 mg/mL (7.74 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.8709 mL 19.3543 mL 38.7087 mL
5 mM 0.7742 mL 3.8709 mL 7.7417 mL
10 mM 0.3871 mL 1.9354 mL 3.8709 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 1 mg/mL (3.87 mM); Clear solution

    此方案可获得 ≥ 1 mg/mL (3.87 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 10.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1 mg/mL (3.87 mM); Clear solution

    此方案可获得 ≥ 1 mg/mL (3.87 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 10.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 1 mg/mL (3.87 mM); Clear solution

    此方案可获得 ≥ 1 mg/mL (3.87 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 10.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Wu J, et al. Two-dimensional gel electrophoretic detection of protein carbonyls derivatized with biotin-hydrazide. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Apr 15;1019:128-31.

Biotin-VAD-FMK

生化分析试剂 Biochemical Assay Reagents
Biotin-VAD-FMK; 纯度: ge;98.0%

Biotin-VAD-FMK是可渗透细胞的,不可逆的,由生物素标记的胱天蛋白酶抑制剂,用于鉴定细胞裂解物中的半胱天冬酶活性。

Biotin-VAD-FMK

Biotin-VAD-FMK Chemical Structure

CAS No. : 1135688-15-1

规格 价格 是否有货 数量
1 mg ¥7000 In-stock
5 mg ¥25000 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Biotin-VAD-FMK 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library

生物活性

Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

IC50 Target

Caspase

;

体外研究
(In Vitro)

Biotin-VAD-FMK is a synthetic peptide designed as a methyl ester to facilitate cell permeability.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

To examine whether caspase-2 is activated in the absence of proteolytic cleavage, an in vivo affinity labeling approach is used, using the biotinylated caspase inhibitor biotin-VAD-fmk that detects only active caspases[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

672.81

Formula

C30H49FN6O8S

CAS 号

1135688-15-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : ≥ 27.3 mg/mL (40.58 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4863 mL 7.4315 mL 14.8630 mL
5 mM 0.2973 mL 1.4863 mL 2.9726 mL
10 mM 0.1486 mL 0.7432 mL 1.4863 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Li J, et al. Nitric oxide suppresses apoptosis via interrupting caspase activation and mitochondrial dysfunction in cultured hepatocytes. J Biol Chem. 1999 Jun 11;274(24):17325-33.

    [2]. Samraj AK, et al. Loss of caspase-9 reveals its essential role for caspase-2 activation and mitochondrial membranedepolarization. Mol Biol Cell. 2007 Jan;18(1):84-93.

荧光染料Biotin-16-dUTP(Synonyms: Biotin-16-deoxyuridine-5′-triphosphate)

荧光染料Dye Reagents Biotin-16-dUTP;(Synonyms: Biotin-16-deoxyuridine-5′-triphosphate)

Biotin-16-dUTP 是 dTTP 的荧光替代物。

Biotin-16-dUTP(Synonyms: Biotin-16-deoxyuridine-5

Biotin-16-dUTP Chemical Structure

CAS No. : 86303-26-6

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Biotin-16-dUTP is a fluorescent substitute of dTTP.

分子量

947.78

Formula

C32H52N7O18P3S

CAS 号

86303-26-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.