思拓凡沃特曼硝酸纤维素膜Protran BA85滤膜10401196

思拓凡沃特曼硝酸纤维素膜Protran BA85滤膜

简要描述:

思拓凡沃特曼硝酸纤维素膜Protran BA85滤膜

 Whatman转印硝酸纤维素膜Western专用10401196/0.45um
*分*纯硝酸纤维素膜
Protran硝酸纤维素(NC)膜是世界上使用*为广泛的特异性转移介质。Protran硝酸纤维素膜采用*分*纯硝酸纤维素材料制成,保证不含醋酸纤维素材料,确保了高的结合能力。其它的硝酸纤维素杂交膜可能含有大量的醋酸纤维素,这会降低蛋白的结合能力。Protran杂交膜采用纯硝酸纤维素制成,具有*好的操作强度,兼容多种检测方法,包括同位素法,化学发光法(以luminol为基础),比色法和荧光法等。与PVDF膜相比,Protran硝酸纤维素膜在使用中不需要甲醇预湿步骤,这使它可以用于那些需要亲水性环境的蛋白质的转移,在转移之前,膜仅需要在水中简单润湿,然后置于转移缓冲液中,而不再需要其它预湿步骤。
 高结合力,低背景
除了具有高结合能力以外,Protran硝酸纤维素膜本身产生的背景非常低,膜*的表面性质保证了优异的信噪比,而同时却不需要严格的清洗条件。小分子蛋白的高保留率
Protran硝酸纤维素膜有多种孔径大小可供选择,使不同的应用都可以获得*佳的效果。0.2μm孔径的Protran BA83硝酸纤维素膜通过减少小分子“下漏”来保留分子量小于20KD的蛋白质分子。0.45μm孔径的Protran BA85硝酸纤维素膜是较大分子量样品和常规核酸研究的理想选择。0.1μm孔径的Protran BA79则用于分子量低于7kD的小分子蛋白转移。
 Protran硝酸纤维素膜的一个主要优势在于,经实验证明,结合在Protran膜上的蛋白能够保持分子识别活性长达5年。
 Protran杂交三明治组合
由1张预切的硝酸纤维素膜和2张3MM层析杂交纸组成的三明治包装,帮您节省更多的时间。这些膜都是用于杂交的质量*好的NC膜——Protran BA83或者BA85。

Protran 硝酸纤维素膜的结合能力为80~150µg/cm2,可高压灭菌(液体冷循环) 

思拓凡沃特曼硝酸纤维素膜Protran BA85滤膜

订货信息:

思拓凡沃特曼硝酸纤维素膜Protran BA85滤膜10401196
思拓凡沃特曼硝酸纤维素膜Protran BA85滤膜10401196



Abaloparatide TFA(Synonyms: 阿巴洛肽 BA 058 TFA BIM 44058 TFA)

Abaloparatide TFA;(Synonyms: 阿巴洛肽; BA 058 TFA; BIM 44058 TFA) 纯度: 96.11%

Abaloparatide TFA (BA 058 TFA) 是一种甲状旁腺激素受体 1 PTHR1 类似物,是 PTHR1 信号通路的有效和选择性激活剂。Abaloparatide TFA 增强 MC3T3-E1 成骨细胞的 Gs/cAMP 信号传导 (EC50 为 0.3 nM) 和 β-arrestin 的募集 (EC50 为 0.9 nM)。

Abaloparatide TFA (Synonyms: 阿巴洛肽; BA 058 TFA; BIM 44058 TFA)

Abaloparatide TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ¥1200 In-stock
5 mg ¥3500 In-stock
10 mg ¥5500 In-stock
25 mg ¥9900 In-stock
50 mg ¥16500 In-stock
100 mg ¥22500 询价
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Abaloparatide TFA 相关产品

相关化合物库:

  • Drug Repurposing Compound Library Plus
  • FDA-Approved Drug Library Plus
  • FDA-Approved Drug Library Mini
  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Abaloparatide TFA (BA 058 TFA) is a parathyroid hormone receptor 1 (PTHR1) analogue selected to be a potent and selective activator of the PTHR1 signaling pathway. Abaloparatide TFA enhances Gs/cAMP signaling (EC50 of 0.3 nM) and β-arrestin recruitment (EC50 of 0.9 nM) in MC3T3-E1 osteoblast cells[1][2].

IC50 & Target

Parathyroid hormone receptor 1 (PTHR1)[1]

体外研究
(In Vitro)

MC3T3-E1 osteoblast cells are treated with 0.01-100 nM of Abaloparatide for 40 min at 37 ℃ in the presence of 0.5 mM IBMX. The results reveals that exposure of cells to Abaloparatide caused a robust elevation of intracellular cAMP levels. Abaloparatide treatment results in a 2.3-fold decrease in EC50 value for cAMP formation compared to teriparatide (EC50s of 0.3 nM and 0.7 nM, respectively)[1].
A dose-dependent stimulation of β-arrestin/PTHR1 interaction is demonstrated by abaloparatide. Consistently, the calculates the EC50 value for abaloparatide is 1.6-fold lower than that of teriparatide (EC50s of 0.9 nM and 1.5 nM, respectively)[1].
Abaloparatide efficiently induces a dose-dependent stimulation of PTHR1 internalization with a dose as low as 0.1 nM and reaches maximum stimulation at 100 nM concentration. The EC50 value of 0.8 nM for Abaloparatide[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Abaloparatide (1-25 µg/kg; subcutaneous injection; daily; for 12 months; female Sprague-Dawley rats) treatment increases biochemical bone formation markers, histomorphometric indices of bone formation on trabecular, endocortical, and periosteal surfaces. Abaloparatide induces substantial increases in trabecular bone volume and density and improvements in trabecular microarchitecture. Abaloparatide stimulates periosteal expansion and endocortical bone apposition at the tibial diaphysis, leading to marked increases in cortical bone volume and density. Whole-body bone mineral density (BMD) is increasing 25% after 12 months of abaloparatide (25 μg/kg) in osteopenic ovariectomized (OVX) rats[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Sprague-Dawley rats (age 22 weeks)[2]
Dosage: 1 µg/kg, 5 µg/kg, 25 µg/kg
Administration: Subcutaneous injection; daily; for 12 months
Result: Increased biochemical bone formation markers, histomorphometric indices of bone formation on trabecular, endocortical, and periosteal surfaces. Induced substantial increases in trabecular bone volume and density and improvements in trabecular microarchitecture. Stimulated periosteal expansion and endocortical bone apposition at the tibial diaphysis, leading to marked increases in cortical bone volume and density. Whole-body bone mineral density (BMD) was increasing 25%.

Clinical Trial

分子量

4074.61

Formula

C176H301N56F3O51

Sequence

Ala-Val-Ser-Glu-His-Gln-Leu-Leu-His-Asp-Lys-Gly-Lys-Ser-Ile-Gln-Asp-Leu-Arg-Arg-Arg-Glu-Leu-Leu-Glu-Lys-Leu-Leu-{Aib}-Lys-Leu-His-Thr-Ala-NH2

Sequence Shortening

AVSEHQLLHDKGKSIQDLRRRELLEKLL-{Aib}-KLHTA-NH2

中文名称

安巴洛他定; 阿巴洛肽; 阿达帕拉肽

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light, stored under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

H2O : ≥ 50 mg/mL (12.27 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2454 mL 1.2271 mL 2.4542 mL
5 mM 0.0491 mL 0.2454 mL 0.4908 mL
10 mM 0.0245 mL 0.1227 mL 0.2454 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Sahbani K, et al. Abaloparatide exhibits greater osteoanabolic response and higher cAMP stimulation and β-arrestin recruitment than teriparatide. Physiol Rep. 2019 Oct;7(19):e14225.

    [2]. Varela A, et al. One Year of Abaloparatide, a Selective Activator of the PTH1 Receptor, Increased Bone Formation and Bone Mass in Osteopenic Ovariectomized Rats Without Increasing Bone Resorption. J Bone Miner Res. 2017 Jan;32(1):24-33.

Whatman 沃特曼 Optitran 加强型硝酸纤维素膜BA-S 83, BA-S 85

Whatman 沃特曼 Optitran 加强型硝酸纤维素膜

简要描述:

型号 :BA-S 83, BA-S 85

Whatman 沃特曼 Optitran 加强型硝酸纤维素膜, BA-S 83, BA-S 85

详细描述

, BA-S 83, BA-S 85

Whatman 加强型硝酸纤维素膜
Optitran?膜是在100%纯硝酸纤维素膜中插入聚酯非纺织材料制成,这种聚酯的支持物并不影响转移条件和效果,同时具备了更强的操作强度,允许反复多重杂交。

Optitran硝酸纤维素膜拥有极低的非特异性结合力,从而保证了更高的灵敏度。使用标准的硝酸纤维素加工流程制成的膜,使用时无需严苛的洗膜和封膜条件。

Optitran膜集合柔韧性、高强度和的信噪比于一体,尤其适合反复杂交实验。

Optitran硝酸纤维素支持型膜灵敏度高,机械强度好,帮您省去更多花费。

Optitran结合能力:75~90μg/cm2,可高压灭菌(液体冷循环)

Whatman 沃特曼 Optitran 加强型硝酸纤维素膜BA-S 83, BA-S 85

, BA-S 83, BA-S 85

10439391, 10439380, 10439394, 10439396, 10439116, 10439126, 10439191, 10439180, 10439194, 10439196

, BA-S 83, BA-S 85

Avermectin B1a 品牌:Biolinks


Avermectin B1a

品牌:Biolinks
CAS No.:65195-55-3
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

BLS-0350

100 μg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

Whatman硝化纤维 转印膜 BA8509301102

简要描述:Whatman硝化纤维 转印膜 BA8509301102

WhatmanProtran 硝化纤维(NC)转印膜

产品型号:

所属分类:转印膜

详情介绍

Whatman硝化纤维 转印膜  BA8509301102

 

硝化纤维转印膜

*纯硝化纤维 保证zui高结合能力 

可用于WESTERN SOUTHERN NORTHERN BLOTTING 的通用膜

可亲水性 无需甲醛浸润

高结合性能 且背景低 高性噪比 无需严格洗涤条件

*的蛋白保留期 膜上的蛋白可保持五年分子识别活跃性

用于较大分子样品量

产品简介

Whatman硝化纤维 转印膜  BA8509301102

Whatman硝化纤维 转印膜  BA8509301102

 

硝化纤维转印膜

*纯硝化纤维 保证zui高结合能力 

可用于WESTERN SOUTHERN NORTHERN BLOTTING 的通用膜

可亲水性 无需甲醛浸润

高结合性能 且背景低 高性噪比 无需严格洗涤条件

*的蛋白保留期 膜上的蛋白可保持五年分子识别活跃性

用于较大分子样品量

产品简介

Whatman硝化纤维 转印膜  BA8509301102

Jena Bioscience PR-BA1373-L Chlamydia abortus (干冰运输) 1mg

上海金畔生物科技有限公司提供Jena Bioscience PR-BA1373-L Chlamydia abortus (干冰运输) 1mg,欢迎访问官网了解更多产品信息和订购

品牌 货号 产品名称 规格 价格

Jena Bioscience PR-BA1373-L Chlamydia abortus (干冰运输) 1mg 26180.00

Jena Bioscience PR-BA1372-S Chlamydia trachomatis (干冰运输) 250µg 8714.00

Jena Bioscience PR-BA1372-L Chlamydia trachomatis (干冰运输) 1mg 26180.00

Jena Bioscience PR-BA1371-S-S Chlamydia pneumoniae Antigen (Premium) (干冰运输) 250µg 13152.00

Jena Bioscience PR-BA1371-S-L Chlamydia pneumoniae Antigen (Premium) (干冰运输) 1mg 39497.00

Jena Bioscience PR-BA1371-S Chlamydia pneumoniae Antigen (干冰运输) 250µg 6668.00

Jena Bioscience PR-BA1371-L Chlamydia pneumoniae Antigen (干冰运输) 1mg 19983.00

Jena Bioscience PR-BA135VS-S Echo Virus Antigen (干冰运输) 250µg 9407.00

Jena Bioscience PR-BA135VS-L Echo Virus Antigen (干冰运输) 1mg 22944.00

Jena Bioscience PR-BA129G2-S Rubella Virus Antigen (干冰运输) 250µg 7853.00

Jena Bioscience PR-BA129G2-L Rubella Virus Antigen (干冰运输) 1mg 23561.00

Jena Bioscience PR-BA128-S Adenovirus Type 2 Hexon Antigen (干冰运输) 250µg 9918.00

Jena Bioscience PR-BA128-L Adenovirus Type 2 Hexon Antigen (干冰运输) 1mg 29780.00

Jena Bioscience PR-BA127-S Mycoplasma pneumoniae Antigen (干冰运输) 250µg 9858.00

Jena Bioscience PR-BA127-L Mycoplasma pneumoniae Antigen (干冰运输) 1mg 29574.00

Jena Bioscience PR-BA1263-S Parainfluenza Virus Type 3 Antigen (干冰运输) 250µg 4970.00

Jena Bioscience PR-BA1263-L Parainfluenza Virus Type 3 Antigen (干冰运输) 1mg 14931.00

Jena Bioscience PR-BA1262-S Parainfluenza Virus Type 2 Antigen (干冰运输) 250µg 4970.00

Jena Bioscience PR-BA1262-L Parainfluenza Virus Type 2 Antigen (干冰运输) 1mg 14931.00

Jena Bioscience PR-BA1261-S Parainfluenza Virus Type 1 Antigen (干冰运输) 250µg 4970.00

Jena Bioscience PR-BA1261-L Parainfluenza Virus Type 1 Antigen (干冰运输) 1mg

上海金畔生物科技有限公司提供Jena Bioscience PR-BA1261-L Parainfluenza Virus Type 1 Antigen (干冰运输) 1mg,欢迎访问官网了解更多产品信息和订购

品牌 货号 产品名称 规格 价格

Jena Bioscience PR-BA1261-L Parainfluenza Virus Type 1 Antigen (干冰运输) 1mg 14931.00

Jena Bioscience PR-BA125-S Leptospira biflexa Antigen (干冰运输) 250µg 7773.00

Jena Bioscience PR-BA125-L Leptospira biflexa Antigen (干冰运输) 1mg 23316.00

Jena Bioscience PR-BA122VP2-S Parvovirus B19 VP2 VLPs (干冰运输) 250µg 10350.00

Jena Bioscience PR-BA122VP2-L Parvovirus B19 VP2 VLPs (干冰运输) 1mg 31070.00

Jena Bioscience PR-BA122VP1-S Parvovirus B19 VP1 (干冰运输) 250µg 8774.00

Jena Bioscience PR-BA122VP1-L Parvovirus B19 VP1 (干冰运输) 1mg 26325.00

Jena Bioscience PR-BA122VP1B-S Parvovirus B19 VP2 C-term (干冰运输) 250µg 8774.00

Jena Bioscience PR-BA122VP1B-L Parvovirus B19 VP2 C-term (干冰运输) 1mg 26325.00

Jena Bioscience PR-BA121-S Borrelia afzelii Antigen (干冰运输) 250µg 6608.00

Jena Bioscience PR-BA121-L Borrelia afzelii Antigen (干冰运输) 1mg 19841.00

Jena Bioscience PR-BA121GVS-S Borrelia garinii Antigen (干冰运输) 250µg 6341.00

Jena Bioscience PR-BA121GVS-L Borrelia garinii Antigen (干冰运输) 1mg 19020.00

Jena Bioscience PR-BA120P-S Pertussis Toxin (干冰运输 危险品 不能进口) 250µg 11535.00

Jena Bioscience PR-BA120P-L Pertussis Toxin (干冰运输 危险品 不能进口) 1mg 40128.00

Jena Bioscience PR-BA120F-S B. pertussis Filamentous Hemagglutinin (FHA) (干冰运输) 250µg 11535.00

Jena Bioscience PR-BA120F-L B. pertussis Filamentous Hemagglutinin (FHA) (干冰运输) 1mg 34605.00

Jena Bioscience PR-BA1193-S Rotavirus Antigen (干冰运输) 250µg 5582.00

Jena Bioscience PR-BA1193-L Rotavirus Antigen (干冰运输) 1mg 16773.00

Jena Bioscience PR-BA118-S Helicobacter pylori Antigen (干冰运输) 250µg 4560.00

Jena Bioscience PR-BA118-L Helicobacter pylori Antigen (干冰运输) 1mg

上海金畔生物科技有限公司提供Jena Bioscience PR-BA118-L Helicobacter pylori Antigen (干冰运输) 1mg,欢迎访问官网了解更多产品信息和订购

品牌 货号 产品名称 规格 价格

Jena Bioscience PR-BA118-L Helicobacter pylori Antigen (干冰运输) 1mg 13704.00

Jena Bioscience PR-BA117-S Candida albicans Antigen (干冰运输) 250µg 3743.00

Jena Bioscience PR-BA117-L Candida albicans Antigen (干冰运输) 1mg 11250.00

Jena Bioscience PR-BA116-S Brucella abortus Antigen (干冰运输) 250µg 4500.00

Jena Bioscience PR-BA116-L Brucella abortus Antigen (干冰运输) 1mg 13500.00

Jena Bioscience PR-BA113-S Respiratory Syncytial Virus (RSV) Antigen (干冰运输) 250µg 5931.00

Jena Bioscience PR-BA113-L Respiratory Syncytial Virus (RSV) Antigen (干冰运输) 1mg 17795.00

Jena Bioscience PR-BA112-S FSME (TBE) Virus Antigen (Premium) (干冰运输) 250µg 10799.00

Jena Bioscience PR-BA112L-S FSME (TBE) Virus Antigen (干冰运输) 250µg 7016.00

Jena Bioscience PR-BA112L-L FSME (TBE) Virus Antigen (干冰运输) 1mg 21066.00

Jena Bioscience PR-BA112-L FSME (TBE) Virus Antigen (Premium) (干冰运输) 1mg 32397.00

Jena Bioscience PR-BA110-S Toxoplasma gondii Antigen (干冰运输) 250µg 9858.00

Jena Bioscience PR-BA110-L Toxoplasma gondii Antigen (干冰运输) 1mg 29574.00

Jena Bioscience PR-BA109-S Cytomegalovirus Antigen (干冰运输) 250µg 7160.00

Jena Bioscience PR-BA109-L Cytomegalovirus Antigen (干冰运输) 1mg 21476.00

Jena Bioscience PR-BA106-S Legionella pneumophila Antigen (干冰运输) 250µg 6465.00

Jena Bioscience PR-BA106-L Legionella pneumophila Antigen (干冰运输) 1mg 19391.00

Jena Bioscience PR-BA1052-S Herpes Simplex Virus (HSV) Type 2 Antigen (干冰运输) 250µg 5114.00

Jena Bioscience PR-BA1052-L Herpes Simplex Virus (HSV) Type 2 Antigen (干冰运输) 1mg 15339.00

Jena Bioscience PR-BA1051-S Herpes Simplex Virus (HSV) Type 1 Antigen (干冰运输) 250µg 5114.00

Jena Bioscience PR-BA1051-L Herpes Simplex Virus (HSV) Type 1 Antigen (干冰运输) 1mg

上海金畔生物科技有限公司提供Jena Bioscience PR-BA1051-L Herpes Simplex Virus (HSV) Type 1 Antigen (干冰运输) 1mg,欢迎访问官网了解更多产品信息和订购

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Jena Bioscience PR-BA1051-L Herpes Simplex Virus (HSV) Type 1 Antigen (干冰运输) 1mg 15339.00

Jena Bioscience PR-BA104VSG-S Varicella Zoster Virus (VZV) Glycoprotein Antigen (干冰运输) 250µg 9941.00

Jena Bioscience PR-BA104VSG-L Varicella Zoster Virus (VZV) Glycoprotein Antigen (干冰运输) 1mg 29780.00

Jena Bioscience PR-BA104-S Varicella Zoster Virus (VZV) Antigen (干冰运输) 250µg 7649.00

Jena Bioscience PR-BA104-L Varicella Zoster Virus (VZV) Antigen (干冰运输) 1mg 22908.00

Jena Bioscience PR-BA103-S Mumps/Parotitis Virus Antigen (干冰运输) 250µg 6176.00

Jena Bioscience PR-BA103-L Mumps/Parotitis Virus Antigen (干冰运输) 1mg 18531.00

Jena Bioscience PR-BA102-S-S Measles Virus Antigen (Premium) (干冰运输) 250µg 6176.00

Jena Bioscience PR-BA102-S-L Measles Virus Antigen (Premium) (干冰运输) 1mg 18531.00

Jena Bioscience PR-BA102-S Measles Virus Antigen (干冰运输) 250µg 4356.00

Jena Bioscience PR-BA102-L Measles Virus Antigen (干冰运输) 1mg 13050.00

Jena Bioscience PR-969S PC7 (干冰运输) 2000U 6771.00

Jena Bioscience PR-969L PC7 (干冰运输) 5 x 2.000U 27087.00

Jena Bioscience PR-968S Furin (干冰运输) 10µg 8246.00

Jena Bioscience PR-968L Furin (干冰运输) 5 x 10µg 32981.00

Jena Bioscience PR-967S Legumain 10µg 3981.00

Jena Bioscience PR-967L Legumain 5 x 10µg 15923.00

Jena Bioscience PR-958S Estradiol Glow 100µg 3488.00

Jena Bioscience PR-958L Estradiol Glow 5x 100µg 9966.00

Jena Bioscience PR-952 FTaseW102T_Y154TGST (干冰运输) 20µg 5010.00

BA 1 TFA

BA 1 TFA; 纯度: 99.65%

BA 1 TFA 是一种 bombesin (BB) 受体家族激动剂。BA 1 与 Bombesin 受体亚型 3 (BRS3),胃泌素释放肽受体 (GRPR),神经调节素 B 受体 (NMBR) 高亲和力结合,IC50 分别为 6、0.4、2.5 nM 。

BA 1 TFAamp;;

BA 1 TFA Chemical Structure

规格 价格 是否有货 数量
5 mg ¥2000 In-stock
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50 mg ; 询价 ;

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BA 1 TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Anti-Cancer Compound Library
  • Targeted Diversity Library
  • Peptide Library

生物活性

BA 1 TFA is a potent agonist for the bombesin (BB) family of receptors. BA1 binds with high affinity to Bombesin receptor subtype-3 (BRS3), gastrin releasing peptide receptor (GRPR), neuromedin B receptor (NMBR) with IC50s of 6, 0.4, 2.5 nM[1].

体外研究
(In Vitro)

BA1 (0.1 μM) significantly increases FAK tyrosine phosphorylation in BRS-3 transfected NCI-H1299 cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1247.32

Formula

C59H77F3N14O13

Sequence Shortening

YQWAV{Bal}HF{Nle}-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Terry W Moody, et al. ML-18 is a non-peptide bombesin receptor subtype-3 antagonist which inhibits lung cancer growth. Peptides. 2015 Feb;64:55-61.