标签归档：fluo

Fluo-3AM(Synonyms: Fluo-3-pentaacetoxymethyl ester)

发表于由

Fluo-3AM;(Synonyms: Fluo-3-pentaacetoxymethyl ester) 纯度: ge;99.0%

Fluo-3AM(Fluo-3-pentaacetoxymethyl ester)是一种钙指示剂，结合Ca2_addition_能增强其荧光，用于流式细胞仪实验涉及光敏化笼状的螯合剂，第二信使和神经递质以及细胞的药理筛选中Ca2 _addition_信号的空间动态成像。

Fluo-3AMamp;;(Synonyms: Fluo-3-pentaacetoxymethyl ester)

Fluo-3AM Chemical Structure

CAS No. : 121714-22-5

规格 价格 是否有货 数量
500 μg ￥3500 In-stock
1 mg ￥5200 In-stock
5 mg ; 询价 ;
10 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Fluo-3AM(Fluo-3-pentaacetoxymethyl ester) is a calcium indicator that exhibit an increase in fluorescence upon binding Ca2+; used to image the spatial dynamics of Ca2+ signaling, in flow cytometry experiments involving photoactivation of caged chelators, second messengers, and neurotransmitters, and for cell-based pharmacological screening.

分子量

1129.85

Formula

C51H50Cl2N2O23
CAS 号

121714-22-5
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 2.5 mg/mL (2.21 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.8851 mL 4.4254 mL 8.8507 mL
5 mM — — —
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

Fluo-4 AM

发表于由

Fluo-4 AM;

Fluo-4 AM 是一种常用的检测细胞内 Ca2+ 浓度的探针。

Fluo-4 AMamp;;

Fluo-4 AM Chemical Structure

CAS No. : 273221-67-3

规格 价格 是否有货
100 μg ￥1500 询问价格 货期

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生物活性

Fluo-4 AM is a cell-permeable Ca2+ indicator[1].

体外研究
(In Vitro)

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Count the cells and take 106 cells from each sample (control and experiment/s).
2. Collect the cells (5 min, 3000×g, 4 °C) and wash once in PBS.
3. Resuspend the cells in 0.5-ml PBS and add 0.5 μl of Fluo-4-AM (1 mM stock) to a final concentration of 1 μM. Incubate at 37 °C for 1 h.
4. Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1-ml PBS. Separate into 2 flow cytometry tubes—0.5 ml in each.
5. Evaluate the staining by flow cytometry and analyze the data by a software such as CellQuest software.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1096.94

Formula

C51H50F2N2O23
CAS 号

273221-67-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, sealed storage, away from moisture and light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献

  • [1]. Gee KR, et al. Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes. Cell Calcium. 2000 Feb;27(2):97-106.

    [2]. Fluo-4.
Cell Assay
[1]

For measuring fluorescence from cells in suspension, dilutions to 2 to 3×106 cells are made, from cultures of rat basophilic leukemia (RBL) cells. Cells are incubated in suspension in 1 μM dye (including Fluo-4 AM) for 30 min at 37°C. Cell suspensions are then transferred to cuvets for measurements of fluorescence emission intensity by spectrofluorometer[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Gee KR, et al. Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes. Cell Calcium. 2000 Feb;27(2):97-106.

    [2]. Fluo-4.

荧光染料Fluo-8 AM

发表于由

荧光染料Dye Reagents Fluo-8 AM;

Fluo-8 AM 是一种钙荧光探针，可以加载到原生质体中检测苹果肉组织细胞中的钙。

Fluo-8 AM

Fluo-8 AM Chemical Structure

CAS No. : 1345980-40-6

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Fluo-8 AM is a calcium fluorescent probe that can be loaded into protoplasts to detect calcium in the flesh tissue cells of Malus domestica[1].

分子量

1046.93

Formula

C50H50N2O23
CAS 号

1345980-40-6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Lina Qiu, et al. Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells of Malus domestica. Hortic Res. 2020 Jun 1;7(1):91.