Notoginsenoside Fe(Synonyms: Notoginseng triterpenes; Ginsenoside Mb)

天然产物 糖类和糖苷 Saccharides and Glycosides

Notoginsenoside Fe;(Synonyms: Notoginseng triterpenes; Ginsenoside Mb) 纯度: 99.94%

三七皂苷Fe(Notoginsenoside Fe)是从珠子参变种中分离的天然化合物。

Notoginsenoside Fe(Synonyms: Notoginseng triterpenes;  Ginsenoside Mb)

Notoginsenoside Fe Chemical Structure

CAS No. : 88105-29-7

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ¥2421 In-stock
5 mg ¥1800 In-stock
10 mg ¥2400 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Notoginsenoside Fe 相关产品

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  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Natural Product Library
  • Glycoside Compound Library
  • Terpenoids Library
  • Traditional Chinese Medicine Monomer Library
  • Food-Sourced Compound Library

生物活性

Notoginsenoside Fe is a natural compound isolated from Panax japlcus var.

分子量

917.13

Formula

C47H80O17

CAS 号

88105-29-7

中文名称

三七皂苷 Fe

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 50 mg/mL (54.52 mM; Need ultrasonic)

DMSO : < 1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble or slightly soluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0904 mL 5.4518 mL 10.9036 mL
5 mM 0.2181 mL 1.0904 mL 2.1807 mL
10 mM 0.1090 mL 0.5452 mL 1.0904 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Li S, et al. Development of a method to screen and isolate potential xanthine oxidase inhibitors from Panax japlcus var via ultrafiltration liquid chromatography combined with counter-current chromatography. Talanta. 2015 Mar;134:665-73.

    [2]. He R, et al. Chemical constituents of leaves of Panax japonicus var. major. Zhongguo Zhong Yao Za Zhi. 2014 May;39(9):1635-8.

Notoginsenoside Fc(Synonyms: 三七皂苷 FC)

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Notoginsenoside Fc (Synonyms: 三七皂苷 FC)

Notoginsenoside Fc 是从三七叶中分离得到的一种原卟啉二醇 (PPD-) 型皂苷,可有效抵消血小板聚集。Notoginsenoside Fc 可以通过促进自噬促进糖尿病大鼠血管损伤后的内皮细胞再生。

Notoginsenoside Fc(Synonyms: 三七皂苷 FC)

Notoginsenoside Fc Chemical Structure

CAS No. : 88122-52-5

规格 价格 是否有货
5 mg ¥2350 询问价格 & 货期
10 mg ¥4000 询问价格 & 货期

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生物活性

Notoginsenoside Fc, a protopanaxadiol- (PPD-) type saponin isolated from the leaves of Panax notoginseng, effectively counteracts platelet aggregation. Notoginsenoside Fc can accelerate reendothelialization following vascular injury in diabetic rats by promoting autophagy[1].

体外研究
(In Vitro)

Notoginsenoside Fc (20 μM; for 24 h) markedly upregulates the expression of LC3B and Beclin 1 and downregulated that of p62 in RAOECs[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Notoginsenoside Fc (3.5 mg/kg/day) accelerates reendothelialization and alleviates excessive neointimal formation following carotid artery injury in diabetic Sprague-Dawley rats (200±20 g) in vivo[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1211.38

Formula

C58H98O26

CAS 号

88122-52-5

中文名称

三七皂苷 FC

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Liu J, et al. Notoginsenoside Fc Accelerates Reendothelialization following Vascular Injury in Diabetic Rats by Promoting Endothelial Cell Autophagy. J Diabetes Res. 2019 Sep 3;2019:9696521.

Notoginsenoside Fa(Synonyms: 三七皂苷FA)

天然产物 糖类和糖苷 Saccharides and Glycosides

Notoginsenoside Fa;(Synonyms: 三七皂苷FA) 纯度: ge;98.0%

Notoginsenoside Fa 是从P. notoginseng 提取出来的一种原人参二醇型皂苷,有激活和恢复退化脑功能的潜能。

Notoginsenoside Fa(Synonyms: 三七皂苷FA)

Notoginsenoside Fa Chemical Structure

CAS No. : 88100-04-3

规格 价格 是否有货 数量
5 mg ¥2020 In-stock
10 mg ¥3430 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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Notoginsenoside Fa 相关产品

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生物活性

Notoginsenoside Fa, a protopanaxadiol (ppd)-type saponin isolated from P. notoginseng, could possibly activate and recover the function of degenerated brain[1][2].

分子量

1241.41

Formula

C59H100O27

CAS 号

88100-04-3

中文名称

三七皂苷FA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (80.55 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.8055 mL 4.0277 mL 8.0554 mL
5 mM 0.1611 mL 0.8055 mL 1.6111 mL
10 mM 0.0806 mL 0.4028 mL 0.8055 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (2.01 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.01 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (2.01 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.01 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (2.01 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.01 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Z.M. Qian, et al. Simultaneous determination of nucleobases, nucleosides and saponins in Panax notoginseng using multiple columns high performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis 48 (2008) 1361-1367.

    [2]. Tohda C, et al. Abeta(25-35)-induced memory impairment, axonal atrophy, and synaptic loss are ameliorated by M1, A metabolite of protopanaxadiol-type saponins. Neuropsychopharmacology. 2004 May;29(5):860-8.

Notoginsenoside R2(Synonyms: 20(S)-Notoginsenoside R2; Ginsenoside Ng-R2)

天然产物 糖类和糖苷 Saccharides and Glycosides

Notoginsenoside R2;(Synonyms: 20(S)-Notoginsenoside R2; Ginsenoside Ng-R2) 纯度: 98.14%

三七皂苷R2(Notoginsenoside R2)是新分离三七皂苷,显示对6-OHDA诱导的氧化应激和细胞凋亡的保护作用。

Notoginsenoside R2(Synonyms: 20(S)-Notoginsenoside R2;  Ginsenoside Ng-R2)

Notoginsenoside R2 Chemical Structure

CAS No. : 80418-25-3

规格 价格 是否有货 数量
1 mg ¥1000 In-stock
5 mg ¥2500 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Notoginsenoside R2 相关产品

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  • Natural Product Library Plus
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  • Oxygen Sensing Compound Library
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  • Traditional Chinese Medicine Monomer Library
  • Neuroprotective Compound Library
  • Food-Sourced Compound Library

生物活性

Notoginsenoside R2 is a newly isolated notoginsenoside from Panax notoginseng, showed neuroprotective effects against 6-OHDA-induced oxidative stress and apoptosis.

分子量

770.99

Formula

C41H70O13

CAS 号

80418-25-3

中文名称

三七皂甙 R2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (129.70 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2970 mL 6.4852 mL 12.9703 mL
5 mM 0.2594 mL 1.2970 mL 2.5941 mL
10 mM 0.1297 mL 0.6485 mL 1.2970 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.24 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.24 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.24 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.24 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.24 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.24 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Meng XB, et al. P90RSK and Nrf2 Activation via MEK1/2-ERK1/2 Pathways Mediated by Notoginsenoside R2 to Prevent 6-Hydroxydopamine-Induced Apoptotic Death in SH-SY5Y Cells. Evid Based Complement Alternat Med. 2013;2013:971712.

    [2]. González-Burgos E, et al. Potential neuroprotective activity of Ginseng in Parkinson’s disease: a review. J Neuroimmune Pharmacol. 2015 Mar;10(1):14-29.

Notoginsenoside S(Synonyms: 三七皂苷S)

天然产物 糖类和糖苷 Saccharides and Glycosides

Notoginsenoside S;(Synonyms: 三七皂苷S)

Notoginsenoside S 是从Panax notoginseng 中分离得到的天然化合物。

Notoginsenoside S(Synonyms: 三七皂苷S)

Notoginsenoside S Chemical Structure

CAS No. : 575446-95-6

规格 价格 是否有货
1 mg ¥1900 询问价格 货期
5 mg ¥5710 询问价格 货期

* Please select Quantity before adding items.

生物活性

Notoginsenoside S is a compound isolated from Panax notoginseng.

分子量

1343.50

Formula

C63H106O30

CAS 号

575446-95-6

中文名称

三七皂苷S

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Notoginsenoside R3

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Notoginsenoside R3 

Notoginsenoside R3 是 P. notoginseng 的活性成分。P. notoginseng 可用作各种生理效应相关的信号分子。P. notoginseng可用作治疗心血管疾病的中药。

Notoginsenoside R3

Notoginsenoside R3 Chemical Structure

CAS No. : 87741-76-2

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100 mg   询价  
250 mg   询价  
500 mg   询价  

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生物活性

Notoginsenoside R3 is an active principle components of P. notoginseng. Notoginsenoside R3 act as a signaling molecule that is associated with various physiological effects. P. notoginseng and its components are used as traditional Chinese medicine for cardiovascular disease[1].

分子量

963.15

Formula

C48H82O19

CAS 号

87741-76-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Chang-Yin Lee, et al. Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng. Oncol Rep. 2017 Jan;37(1):399-407.

20(R)-Notoginsenoside R2(Synonyms: 20(R)-三七皂苷R)

天然产物 糖类和糖苷 Saccharides and Glycosides

20(R)-Notoginsenoside R2;(Synonyms: 20(R)-三七皂苷R)

20(R)-Notoginsenoside R2 是一种分离自 Panax notoginseng 的皂苷。

20(R)-Notoginsenoside R2(Synonyms: 20(R)-三七皂苷R)

20(R)-Notoginsenoside R2 Chemical Structure

CAS No. : 948046-15-9

规格 价格 是否有货
5 mg ¥3360 询问价格 货期
10 mg ¥5710 询问价格 货期

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生物活性

20(R)-Notoginsenoside R2 is an isolated notoginsenoside from Panax notoginseng[1].

分子量

770.99

Formula

C41H70O13

CAS 号

948046-15-9

中文名称

20(R)-三七皂苷R

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Chen G, et al. Microbial transformation of 20(S)-protopanaxatriol-type saponins by Absidia coerulea. J Nat Prod. 2007 Jul;70(7):1203-6. Epub 2007 Jul 13.

Notoginsenoside T5

天然产物 糖类和糖苷 Saccharides and Glycosides

Notoginsenoside T5;

Notoginsenoside T5 是一种 dammarane 61 glycoside. Notoginsenoside T5 是从 P. 62 notoginseng 的根皂苷酸性脱糖基中分离出来的。

Notoginsenoside T5

Notoginsenoside T5 Chemical Structure

CAS No. : 769932-34-5

规格 是否有货
5 mg 询价
10 mg 询价
25 mg 询价

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生物活性

Notoginsenoside T5 is a dammarane 61 glycoside. Notoginsenoside T5 is isolated from the acidic deglycosylation of saponins from the roots of P. 62 notoginseng[1].

分子量

752.97

Formula

C41H68O12

CAS 号

769932-34-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Wang RF, et al. Chemical transformation and target preparation of saponins in stems and leaves of Panax notoginseng. J Ginseng Res. 2018;42(3):270-276.

Notoginsenoside FP2(Synonyms: 三七皂苷FP2)

天然产物 糖类和糖苷 Saccharides and Glycosides

Notoginsenoside FP2;(Synonyms: 三七皂苷FP2)

Notoginsenoside FP2 是从 Panax notoginseng 果梗中分离得到的,具有研究心血管疾病的潜能。

Notoginsenoside FP2(Synonyms: 三七皂苷FP2)

Notoginsenoside FP2 Chemical Structure

CAS No. : 1004988-75-3

规格 价格 是否有货
5 mg ¥3360 询问价格 货期
10 mg ¥5710 询问价格 货期

* Please select Quantity before adding items.

生物活性

Notoginsenoside FP2, a dammarane-Type Bisdesmoside isolated from the Fruit Pedicels of Panax notoginseng, has potential to treat cardiovascular disease[1][2].

分子量

1211.38

Formula

C58H98O26

CAS 号

1004988-75-3

中文名称

三七皂苷FP2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Xian-You Wang, et al. Two New Dammarane-Type Bisdesmosides from the Fruit Pedicels of Panax notoginseng. Helvetica Chimica Acta -Vol. 91 (2008).

    [2]. Liu J, et al. Saponins of Panax notoginseng: chemistry, cellular targets and therapeutic opportunities in cardiovascular diseases. Expert Opin Investig Drugs. 2014 Apr;23(4):523-39.

Notoginsenoside Ft1(Synonyms: 三七皂甙 Ft1)

天然产物 糖类和糖苷 Saccharides and Glycosides

Notoginsenoside Ft1;(Synonyms: 三七皂甙 Ft1) 纯度: ge;98.0%

三七皂苷Ft1(Notoginsenoside Ft1)是植物分离三七皂苷,刺激血管生成。

Notoginsenoside Ft1(Synonyms: 三七皂甙 Ft1)

Notoginsenoside Ft1 Chemical Structure

CAS No. : 155683-00-4

规格 价格 是否有货 数量
5 mg ¥800 In-stock
10 mg ¥1000 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Notoginsenoside Ft1 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Natural Product Library
  • Glycoside Compound Library
  • Terpenoids Library

生物活性

Notoginsenoside Ft1 is a saponin isolated from Panax notoginseng; stimulator of angiogenesis. IC50 value: Target: angiogenesis stimulator in vitro: Ft1 increases translocalization of hypoxia-inducible factor-1α (HIF-1α) from cytoplasm to nuclei, where it binds to the vascular endothelial growth factor (VEGF) promoter, increasing the expression of VEGF mRNA and the subsequent secretion of the growth factor. Ft1 induces the activation of PI3K/AKT and Raf/MEK/ERK signaling pathways [1]. Among the saponins examined, Ft1 was the most potent procoagulant and induced dose-dependent platelet aggregation. Ft1 reduced plasma coagulation indexes, decreased tail bleeding time and increased thrombogenesis. Moreover, it potentiated ADP-induced platelet aggregation and increased cytosolic Ca(2+) accumulation, effects that were attenuated by clopidogrel. Ft1 binds to platelet P2Y12 receptors. The increase in intracellular Ca(2+) evoked by Ft1 in HEK293 cells overexpressing P2Y12 receptors could be blocked by ticagrelor [2]. Ft1 caused endothelium-dependent relaxations, which were abolished by l-NAME (inhibitor of nitric oxide synthases) and ODQ (inhibitor of soluble guanylyl cyclase). Ft1 increased the cGMP level in rat mesenteric arteries. GR and ER were present in the endothelial layer and their antagonism by RU486 and PHTPP, respectively, inhibited Ft1-induced endothelium-dependent relaxations and phosphorylations of eNOS, Akt and ERK1/2 [3]. Ft1 showed the best inhibitory effect on cell proliferation of SH-SY5Y cells with IC50 of 45μM. Ft1 not only arrested the cell cycle at S, G2/M stages, but also promoted cell apoptosis. Ft1 up-regulated the protein expressions of cleaved caspase 3, phospho-p53, p21, and cyclin B1, but down-regulated that of Bcl-2. Moreover, Ft1 enhanced the phosphorylation of ERK1/2, JNK and p38 MAPK [4]. in vivo: Ft1 promotes the formation of blood vessels in Matrigel plug and wound healing in mice [1].

分子量

917.13

Formula

C47H80O17

CAS 号

155683-00-4

中文名称

三七皂甙 Ft1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (stored under nitrogen)

溶解性数据
In Vitro:;

DMSO : 6.67 mg/mL (7.27 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0904 mL 5.4518 mL 10.9036 mL
5 mM 0.2181 mL 1.0904 mL 2.1807 mL
10 mM

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 0.67 mg/mL (0.73 mM); Clear solution

    此方案可获得 ≥ 0.67 mg/mL (0.73 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 6.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.67 mg/mL (0.73 mM); Clear solution

    此方案可获得 ≥ 0.67 mg/mL (0.73 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 6.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 0.67 mg/mL (0.73 mM); Clear solution

    此方案可获得 ≥ 0.67 mg/mL (0.73 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 6.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Shen K, et al. Notoginsenoside Ft1 promotes angiogenesis via HIF-1α mediated VEGF secretion and the regulation of PI3K/AKT and Raf/MEK/ERK signaling pathways. Biochem Pharmacol. 2012 Sep 15;84(6):784-92.

    [2]. Gao B, et al. Platelet P2Y?? receptors are involved in the haemostatic effect of notoginsenoside Ft1, a saponin isolated from Panax notoginseng. Br J Pharmacol. 2014 Jan;171(1):214-23.

    [3]. Shen K, et al. Notoginsenoside Ft1 activates both glucocorticoid and estrogen receptors to induce endothelium-dependent, nitric oxide-mediated relaxations in rat mesenteric arteries. Biochem Pharmacol. 2014 Mar 1;88(1):66-74.

    [4]. Gao B, et al. p38 MAPK and ERK1/2 pathways are involved in the pro-apoptotic effect of notoginsenoside Ft1 on human neuroblastoma SH-SY5Y cells. Life Sci. 2014 Jul 17;108(2):63-70.