Biotin-azide(Synonyms: N-(3-Azidopropyl)biotinamide)

生化分析试剂 Biochemical Assay Reagents
Biotin-azide;(Synonyms: N-(3-Azidopropyl)biotinamide) 纯度: 99.64%

Biotin-azide (N-(3-Azidopropyl)biotinamide) 是具有末端叠氮基团的生物素的一种形式。Biotin-azide 用于通过 Click Chemistry 制备各种生物素化的缀合物。

Biotin-azide(Synonyms: N-(3-Azidopropyl)biotinamide)

Biotin-azide Chemical Structure

CAS No. : 908007-17-0

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1500 In-stock
5 mg ¥1360 In-stock
10 mg ¥1800 In-stock
25 mg ¥3200 In-stock
50 mg ¥5400 In-stock
100 mg ¥8500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Biotin-azide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Biotin-azide (N-(3-Azidopropyl)biotinamide) is a form of biotin with a terminal azide group. Biotin-azide can be used to prepare various biotinylated conjugates via Click Chemistry[1][2].

分子量

326.42

Formula

C13H22N6O2S

CAS 号

908007-17-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 250 mg/mL (765.88 mM; Need ultrasonic)

H2O : 4 mg/mL (12.25 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.0635 mL 15.3177 mL 30.6354 mL
5 mM 0.6127 mL 3.0635 mL 6.1271 mL
10 mM 0.3064 mL 1.5318 mL 3.0635 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.37 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.37 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.37 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Bruckman MA, et al. Tobacco mosaic virus-based protein nanoparticles and nanorods for chemotherapy delivery targeting breast cancer. J Control Release. 2016;231:103‐113.

    [2]. Kim HY, et al. An azido-biotin reagent for use in the isolation of protein adducts of lipid-derived electrophiles by streptavidin catch and photorelease. Mol Cell Proteomics. 2009;8(9):2080‐2089.

荧光染料Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide

荧光染料Dye Reagents Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide; 纯度: 95.02%

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide 是一种可点击、酸裂解的 Biotin-picolyl azide。 Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide 是细胞表面糖蛋白的富集手柄,可用于蛋白质标记。

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azideamp;;

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide Chemical Structure

CAS No. : 2599839-59-3

规格 价格 是否有货 数量
5 mg ¥3900 In-stock
10 mg ¥6200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide is a clickable, acid-cleavable biotin-picolyl azide. Biotin-PEG4-dialkoxydiphenylsilane-picolyl azide is an enrichment handle of cell surface glycoproteins for protein labeling[1].

分子量

978.24

Formula

C47H67N9O10SSi

CAS 号

2599839-59-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (stored under nitrogen)

参考文献
  • [1]. Benjamin Schumann, et al. Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells. Mol Cell. 2020 Jun 4;78(5):824-834.e15.

Janelia Fluor® 549, Azide(Synonyms: JF549, Azide)

Janelia Fluor® 549, Azide;(Synonyms: JF549, Azide)

Janelia Fluor® 549, Azide (JF549, Azide) 是一种荧光染料,最大吸收波长 (λab (max)) 为 549 nm,最大发射波长 (λem (max)) 为 571 nm。Janelia Fluor® 在美国专利下获得许可,来自 Howard Hughes Medical Institute 的 No.9,933,417,10,018,624,10,161,932 和其他专利。

Janelia Fluor® 549, Azideamp;;(Synonyms: JF549, Azide)

Janelia Fluor® 549, Azide Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Janelia Fluor® 549, Azide (JF549, Azide) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

分子量

654.71

Formula

C35H38N6O7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Jonathan B Grimm, et al. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol Biol. 2017;1663:179-188.

Janelia Fluor® 646, Azide(Synonyms: JF646, Azide)

Janelia Fluor® 646, Azide;(Synonyms: JF646, Azide)

Janelia Fluor® 646, Azide (JF646, Azide) 是一种红色荧光染料,含有 click chemistry 基团叠氮化物。Janelia Fluor® 646, Azide 可用于活细胞成像实验中。Janelia Fluor® 产品在美国专利下获得许可,来自 Howard Hughes Medical Institute 的 No.9,933,417,10,018,624,10,161,932 和其他专利。

Janelia Fluor® 646, Azideamp;;(Synonyms: JF646, Azide)

Janelia Fluor® 646, Azide Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Janelia Fluor® 646, Azide (JF646, Azide) is a red fluorogenic fluorescent dye containing a click chemistry group Azide. Janelia Fluor® 646, Azide can be used for live-cell imaging experiments[1][2]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

体外研究
(In Vitro)

Maximum absorption wavelength (λabs)= 646 nm, maximum emission wavelength (λem)=664 nm[1].
JF646 can serve as a ligand for self-labeling tag (such as HaloTag)[1].
Janelia Fluor® 646, Azide is modified by JF646. JF646, a red fluorescent dye, is photostable, membrane-permeable, has a high extinction coefficient[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

696.87

Formula

C37H44N6O6Si

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Grimm JB, Muthusamy AK, Liang Y, et al. A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat Methods. 2017;14(10):987-994. doi:10.1038/nmeth.4403.

    [2]. Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28.