Enhanced Green Fluorescent Protein (EGFP) (200-208)

Enhanced Green Fluorescent Protein (EGFP) (200-208);

Enhanced Green Fluorescent Protein (EGFP) (200-208) 是一种标记基因产物,源自维多利亚水母 (Aequorea Victoria)。Enhanced Green Fluorescent Protein (EGFP) (200-208) 是一种常见的报告蛋白,易于被检测到。

Enhanced Green Fluorescent Protein (EGFP) (200-208)amp;;

Enhanced Green Fluorescent Protein (EGFP) (200-208) Chemical Structure

CAS No. : 323198-39-6

规格 是否有货
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生物活性

Enhanced Green Fluorescent Protein (EGFP) (200-208) is a marker gene product derived from the jellyfish Aequorea Victoria. Enhanced Green Fluorescent Protein (EGFP) (200-208) is a common reporter protein and is easy to detect[1].

分子量

1019.11

Formula

C45H70N12O15

CAS 号

323198-39-6

Sequence Shortening

HYLSTQSAL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. J Steitz, et al. Balb/c EGFP mice are tolerant against immunization utilizing recombinant adenoviral-based vectors encoding EGFP: a novel model for the study of tolerance mechanisms and vaccine efficacy. Mol Immunol. 2010 Feb;47(5):1149-53.

荧光染料Green DND-26

荧光染料Dye Reagents Green DND-26;

Green DND-26 用于标记酸性溶酶体以确定细胞分布。Green DND-26 是一种荧光染料,可对活细胞中的酸性隔室进行染色,并已被证明可选择性地积聚在肺泡 II 型 (AT2) 细胞的层状体中。

Green DND-26

Green DND-26 Chemical Structure

CAS No. : 220524-71-0

规格 是否有货
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生物活性

Green DND-26 is used to mark acidic lysosomes to determine the cellular distribution. Green DND-26 is a fluorescent dye that stains acidic compartments in live cells and has been shown to selectively accumulate in lamellar bodies in alveolar type II (AT2) cells in the lung[1][2].

分子量

362.23

Formula

C18H25BF2N4O

CAS 号

220524-71-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Sun B, et al. Acid-Activatable Transmorphic Peptide-Based Nanomaterials for Photodynamic Therapy. Angew Chem Int Ed Engl. 2020;59(46):20582-20588.

    [2]. Van der Velden JL, et al. LysoTracker is a marker of differentiated alveolar type II cells. Respir Res. 2013;14(1):123. Published 2013 Nov 11.

Indocyanine green(Synonyms: 吲哚菁绿 Foxgreen IC Green)

Indocyanine green;(Synonyms: 吲哚菁绿; Foxgreen; IC Green) 纯度: ge;98.0%

Indocyanine green (Foxgreen) 是一种低毒性荧光剂,广泛用于医学诊断,如确定心输出量,肝功能和肝血流量,以及用于眼科血管造影。

Indocyanine greenamp;;(Synonyms: 吲哚菁绿; Foxgreen;  IC Green)

Indocyanine green Chemical Structure

CAS No. : 3599-32-4

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ¥1390 In-stock
100 mg ¥840 In-stock
500 mg ¥3360 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

Indocyanine green 相关产品

bull;相关化合物库:

  • Rare Diseases Drug Library
  • Bioactive Compound Library Plus
  • Drug-Induced Liver Injury (DILI) Compound Library

生物活性

Indocyanine green (Foxgreen) is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography.

体外研究
(In Vitro)

Indocyanine green (Foxgreen)-photodynamic therapy (ICG-PDT) at concentrations 1000 μg/mL, induces the significant expression of BAX in HGF cells; however, the laser irradiation as well as ICG shows no significant effects on the expression of BCL-2 gene[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

774.96

Formula

C43H47N2NaO6S2

CAS 号

3599-32-4

中文名称

靛氰绿;吲哚菁绿

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 33.33 mg/mL (43.01 mM; Need ultrasonic)

H2O : 5 mg/mL (6.45 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2904 mL 6.4519 mL 12.9039 mL
5 mM 0.2581 mL 1.2904 mL 2.5808 mL
10 mM 0.1290 mL 0.6452 mL 1.2904 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.23 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.23 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (3.23 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (3.23 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Gharesi S, et al. Effect of photodynamic therapy based on indocyanine green on expression of apoptosis-related genes in human gingival fibroblast cells. Photodiagnosis Photodyn Ther. 2017 Apr 21.

荧光染料MitoTracker Green FM

荧光染料Dye Reagents MitoTracker Green FM;

MitoTracker Green FM 是一种绿色荧光染料,可以选择性地积聚在线粒体基质中。MitoTracker Green FM 通过与半胱氨酸残基的游离硫醇基反应共价结合线粒体蛋白。

MitoTracker Green FM

MitoTracker Green FM Chemical Structure

CAS No. : 201860-17-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

MitoTracker Green FM is a green-fluorescent dye that can selectively accumulate in the mitochondrial matrix. MitoTracker Green FM covalently binds to mitochondrial proteins by reacting with free thiol groups of cysteine residues[1].

分子量

671.87

Formula

C34H28Cl5N3O

CAS 号

201860-17-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Gautam N, et, al. A high content imaging flow cytometry approach to study mitochondria in T cells: MitoTracker Green FM dye concentration optimization. Methods. 2018 Feb 1;134-135:11-19.

荧光染料ER-Tracker Green

荧光染料Dye Reagents ER-Tracker Green;

ER-Tracker Green 是一种内质网特异性的荧光染料。

ER-Tracker Green

ER-Tracker Green Chemical Structure

CAS No. : 730931-46-1

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
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生物活性

ER-Tracker Green is a fluorescent dye that specific for endoplasmic reticulum[1].

体外研究
(In Vitro)

ER tracker Green (1 mM, incubated in OptiMEM for 30 min at 37 °C) is used for endoplasmic reticulum staining[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

783.09

Formula

C37H42BClF2N6O6S

CAS 号

730931-46-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. J Jacob Strouse, et al. Fluorescent substrates for flow cytometric evaluation of efflux inhibition in ABCB1, ABCC1, and ABCG2 transporters. Anal Biochem. 2013 Jun 1;437(1):77-87.

荧光染料Sel-green

荧光染料Dye Reagents Sel-green;

Sel-green 是一种选择性的硒醇荧光探针,用于定量硒酶硫氧蛋白还原酶中的 Sec 含量,并对活细胞中的 Sec 进行荧光呈像。

Sel-green

Sel-green Chemical Structure

CAS No. : 1574299-37-8

规格 价格 是否有货
5 mg ¥3500 询问价格 货期
10 mg ¥5800 询问价格 货期

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生物活性

Sel-green, a selective selenol fluorescent probe, is applied to quantify the Sec content in the selenoenzyme thioredoxin reductase and image endogenous Sec in live HepG2 cells[1].

体外研究
(In Vitro)

Sel-green displays >20-fold selectivity of Sec over DTT[1]. Sel-green is suitable for identifying the selenol species metabolized from various selenocompounds in cells, which helps to clarify the mechanism underlying the cytotoxicity of different selenocompounds[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

462.43

Formula

C19H18N4O8S

CAS 号

1574299-37-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

溶解性数据
In Vitro:;

DMSO : 50 mg/mL (108.12 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1625 mL 10.8124 mL 21.6249 mL
5 mM 0.4325 mL 2.1625 mL 4.3250 mL
10 mM 0.2162 mL 1.0812 mL 2.1625 mL

参考文献
  • [1]. Baoxin Zhang, et al. Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applications. J Am Chem Soc. 2015 Jan 21;137(2):757-69.

荧光染料PMQA(Synonyms: Zn-green)

荧光染料Dye Reagents PMQA;(Synonyms: Zn-green)

PMQA (Zn-green) 是一种基于 8-aminoquinoline 的比率荧光传感器,显示了 Zn2+ 诱导的辐射红移 (85 nm)。PMQA (Zn-green) 是一种具有细胞膜通透性的荧光探针,适用于活细胞中的 Zn2+ 检测。

PMQA(Synonyms: Zn-green)

PMQA Chemical Structure

CAS No. : 1263820-18-3

规格 价格 是否有货
5 mg ¥2500 询问价格 货期
10 mg ¥4000 询问价格 货期

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生物活性

PMQA (Zn-green), an 8-aminoquinoline-based ratiometric fluorescent sensor, demonstrates the Zn2+-induced redshift of emission (85 nm). PMQA (Zn-green) is a cell membrane-permeable probe and suitable for imaging Zn2+ in living cells[1].

体外研究
(In Vitro)

After incubation with PMQA (20 μM) for 1 h, weak green fluorescence appeared, presumably caused by the probe capturing intracellular zinc from its native ligands since PMQA has high affinity for zinc[1].
The response of PMQA to Zn2+ can be reversed by N,N,N’,N’-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) or EDTA[1].
When the stock solution of PMQA (0.1 mM in Tris Buffer) is kept at room temperature for a week, there is no significant loss of PMQA determined by HPLC analysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

292.34

Formula

C17H16N4O

CAS 号

1263820-18-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Liangwei Zhang, et al. 8-Aminoquinoline-based ratiometric zinc probe: unexpected binding mode and its application in living cells. Bioorg Med Chem Lett. 2013 Jun 15;23(12):3511-4.

荧光染料NEP(Synonyms: VDP-green (NEP))

荧光染料Dye Reagents NEP;(Synonyms: VDP-green (NEP))

NEP (VDP-green (NEP)) 是一种基于分子内电荷转移 (ICT) 机制的感应荧光探针,用于感应含邻二硫醇的蛋白质 (VDPs)。NEP 在活细胞和动物体内对 VDPs 表现出高选择性,并显示强绿色荧光信号 (λexem=430/535 nm)。NEP 具有用于帕金森氏症研究的潜力。

NEP(Synonyms: VDP-green (NEP))

NEP Chemical Structure

CAS No. : 2414276-32-5

规格 价格 是否有货
5 mg ¥3500 询问价格 货期
10 mg ¥5800 询问价格 货期

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生物活性

NEP (VDP-green (NEP)) is a turn-on fluorescent probe based on the intramolecular charge transfer (ICT) mechanism for sensing vicinal dithiol-containing proteins (VDPs). NEP exhibits high selectivity toward VDPs in live cells and in vivo and displays a strong green fluorescence signal (λexem=430/535 nm). NEP has the potential for parkinsonism[1].

体外研究
(In Vitro)

NEP (1-30 μM; 6 hours) has no or little cytotoxicity in HepG2 and PC12 cells[1].
NEP (10 μM; 4 hours) causes the fluorescence intensity to decrease gradually in PC12 cells pretreated with 6-OHDA (0, 50, 100, and 200 μM; for 30 min)[1].
NEP contains a dithiarsolane moiety (five-membered As-S ring) as the receptor of VDPs. In the presence of VDPs, NEP displays a strong green fluorescence signal produced by the cyclic dithiarsolane cleavage and subsequent intramolecular cyclization to liberate the fluorophore. NEP maintains a reliable fluorescence response within the range of pH 7-10[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

NEP (10 μM; for 4 h) causes the obvious green signal in zebrafishes (4 day old)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

654.63

Formula

C29H31AsN4O5S2

CAS 号

2414276-32-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Guodong Hu, et al. Decrease of Protein Vicinal Dithiols in Parkinsonism Disclosed by a Monoarsenical Fluorescent Probe. Anal Chem. 2020 Mar 17;92(6):4371-4378.

荧光染料AC-green(Synonyms: VDP-green)

荧光染料Dye Reagents AC-green;(Synonyms: VDP-green)

AC-green (VDP-green) 是一种 β-烯丙基氨基甲酸酯荧光探针,可选择性地对活体系统中的邻位二硫醇蛋白质 (VDPs) 进行成像 (λex/λem=400/475 nm)。AC-green 可以高灵敏度检测降低牛血清白蛋白 (rBSA)。AC-green 毒性低,灵敏度高,适用于检测活细胞和斑马鱼中的 VDP。

AC-green(Synonyms: VDP-green)

AC-green Chemical Structure

规格 价格 是否有货
5 mg ¥3500 询问价格 货期
10 mg ¥5800 询问价格 货期

* Please select Quantity before adding items.

生物活性

AC-green (VDP-green) is a β-allyl carbamate fluorescent probe for specifically imaging vicinal dithiol proteins (VDPs) in living systems (λexem=400/475 nm). AC-green can detect the reduced bovine serum albumin (rBSA) with high sensitivity. AC-green displays low toxicity and features high sensitivity, and is suitable for sensing VDPs in living cells and zebrafishes[1].

体外研究
(In Vitro)

AC-green (VDP-green) can respond to VDPs with more than 60-fold increase of emission in aqueous solution, while there is no significant interference from biological thiols, amino acids or inorganic salts[1].
AC-green (2 μM; 90 min) has no apparent fluorescence signal within the pH range of 5.0-9.0. Addition of rBSA turns on the fluorescence[1].
AC-gree (10 μM) has low cytotoxicity in HepG2 cells and Hela cells[1].
AC-gree (10 μM; for 15 min) images VDPs in living HepG2 cells and bright green fluorescence appeared. This fluorescence is inhibited when the cells are pretreated with PAO, a popular specificligand for protein vicinal dithiols[1].
AC-gree (10 μM; for 20 min) incubates with zebrafishes has a strong fluorescence signal appeared in the green channel[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

435.47

Formula

C24H25N3O5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Lanning Zhao, et al. A β-allyl carbamate fluorescent probe for vicinal dithiol proteins. Chem Commun (Camb). 2020 Mar 5;56(19):2857-2860.

Fast Green FCF(Synonyms: FD&C Green No. 3 Food green 3 C.I. 42053)

Fast Green FCF;(Synonyms: FDC Green No. 3; Food green 3; C.I. 42053) 纯度: 96.45%

Fast Green FCF是一种海绿色三芳基甲烷食用染料, 可在电泳时进行蛋白质染色, 最大吸收在625nm处。

Fast Green FCFamp;;(Synonyms: FDamp;C Green No. 3;  Food green 3;  C.I. 42053)

Fast Green FCF Chemical Structure

CAS No. : 2353-45-9

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ¥500 In-stock
500 mg ¥400 In-stock
1 g ¥500 In-stock
5 g ; 询价 ;
10 g ; 询价 ;

* Please select Quantity before adding items.

Fast Green FCF 相关产品

bull;相关化合物库:

  • Food Additive Library
  • Inactive Ingredient Library
  • Bioactive Compound Library Plus

生物活性

Fast Green FCF is a sea green triarylmethane food dye. Fast Green FCF is used as a quantitative stain for histones at alkaline pH after acid extraction of DNA. It is also used as a protein stain in electrophoresis. Its absorption maximum is at 625 nm.

分子量

808.85

Formula

C37H34N2Na2O10S3

CAS 号

2353-45-9

中文名称

固绿 FCF;坚牢绿;坚牢绿 FCF;食用色素绿色 3 号;固绿 FCF;快绿;食品绿 3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

H2O : 10 mg/mL (12.36 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2363 mL 6.1816 mL 12.3632 mL
5 mM 0.2473 mL 1.2363 mL 2.4726 mL
10 mM 0.1236 mL 0.6182 mL 1.2363 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。