RFRP-3(human) TFA(Synonyms: Neuropeptide VF(124-131)(human) TFA)

RFRP-3(human) TFA;(Synonyms: Neuropeptide VF(124-131)(human) TFA)

RFRP-3 TFA efficiently inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM.
Scatchard-plot analysis shows that 125I-labelled hRFRP-3 has a single class of high-affinity binding sites for the membrane fractions of CHO cells expressing rat OT7T022, the Kd value and the Bmax values are 0.19 nM and 1.3 pM, respectively.
RFRP-3 TFA specifically stimulate cells transfected with a new orphan 7TMR, OT7T022, it binds to OT7T022 as a specific ligand with high affinity (Kd= 0.19 nM).
RFRP-3 (10-8 to 10-14M) has no effect on LH and FSH levels alone, but when it combines with GnRH, LH and FSH secretion is significantly reduced by the combination.

RFRP-3(human) TFAamp;;(Synonyms: Neuropeptide VF(124-131)(human) TFA)

RFRP-3(human) TFA Chemical Structure

规格 是否有货
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RFRP-3(human) TFA 的其他形式现货产品:

RFRP-3(human)

生物活性

RFRP-3 (Neuropeptide VF(124-131))(human) TFA, a human GnIH peptide homolog, is a potent inhibitor of gonadotropin secretion by inhibiting Ca2+ mobilization. RFRP-3(human) is a NPFF1 receptor agonist, it inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM[1].

体外研究
(In Vitro)

RFRP-3 efficiently inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM[1].
Scatchard-plot analysis shows that 125I-labelled hRFRP-3 has a single class of high-affinity binding sites for the membrane fractions of CHO cells expressing rat OT7T022, the Kd value and the Bmax values are 0.19 nM and 1.3 pM, respectively.
RFRP-3 TFA specifically stimulate cells transfected with a new orphan 7TMR, OT7T022, it binds to OT7T022 as a specific ligand with high affinity (Kd= 0.19 nM)[1].
RFRP-3 TFA (10-8 to 10-14M) has no effect on LH and FSH levels alone, but when it combines with GnRH, LH and FSH secretion is significantly reduced by the combination[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1083.16

Formula

C47H73F3N14O12

Sequence Shortening

VPNLPQRF-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Iain J Clarke, et al. Potent action of RFamide-related peptide-3 on pituitary gonadotropes indicative of a hypophysiotropic role in the negative regulation of gonadotropin secretion. Endocrinology

RFRP-1(human) TFA

RFRP-1(human) TFA;

RFRP-1(human) TFA is a potent endogenous NPFF receptor agonist (EC50 values are 0.0011 and 29 nM for NPFF2 and NPFF1, respectively). Attenuates contractile function of isolated rat and rabbit cardiac myocytes. Reduces heart rate, stroke volume, ejection fraction and cardiac output, and increases plasma prolactin levels in rats.

RFRP-1(human) TFAamp;;

RFRP-1(human) TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

RFRP-1(human) TFA is a potent endogenous NPFF receptor agonist (EC50 values are 0.0011 and 29 nM for NPFF2 and NPFF1, respectively). Attenuates contractile function of isolated rat and rabbit cardiac myocytes. Reduces heart rate, stroke volume, ejection fraction and cardiac output, and increases plasma prolactin levels in rats.

分子量

1542.74

Formula

C62H102F3N19O16S

Sequence Shortening

MPHSFANLPLRF-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

RFRP-3(human)(Synonyms: Neuropeptide VF(124-131)(human))

RFRP-3(human);(Synonyms: Neuropeptide VF(124-131)(human)) 纯度: 98.51%

RFRP-3 (Neuropeptide VF(124-131))(human),一种人GnIH 肽同源物,是一种促性腺激素分泌抑制 Ca2+ 动员的有效抑制剂。RFRP-3(human) 是一种NPFF1 受体激动剂,它抑制 forskolin (HY-15371) 诱导的 cAMP 生成的 IC50 值为 0.7 nM。

RFRP-3(human)amp;;(Synonyms: Neuropeptide VF(124-131)(human))

RFRP-3(human) Chemical Structure

CAS No. : 311309-27-0

规格 价格 是否有货 数量
1 mg ¥1400 In-stock
5 mg ¥4900 In-stock
10 mg ¥7800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

RFRP-3 (Neuropeptide VF(124-131))(human), a human GnIH peptide homolog, is a potent inhibitor of gonadotropin secretion by inhibiting Ca2+ mobilization. RFRP-3(human) is a NPFF1 receptor agonist, it inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM[1].

体外研究
(In Vitro)

RFRP-3 efficiently inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM[1].
Scatchard-plot analysis shows that 125I-labelled hRFRP-3 has a single class of high-affinity binding sites for the membrane fractions of CHO cells expressing rat OT7T022, the Kd value and the Bmax values are 0.19 nM and 1.3 pM, respectively.
RFRP-3 specifically stimulate cells transfected with a new orphan 7TMR, OT7T022, it binds to OT7T022 as a specific ligand with high affinity (Kd= 0.19 nM)[1].
RFRP-3 (10-8 to 10-14M) has no effect on LH and FSH levels alone, but when it combines with GnRH, LH and FSH secretion is significantly reduced by the combination[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

969.14

Formula

C45H72N14O10

CAS 号

311309-27-0

Sequence Shortening

VPNLPQRF-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : ≥ 100 mg/mL (103.18 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0318 mL 5.1592 mL 10.3184 mL
5 mM 0.2064 mL 1.0318 mL 2.0637 mL
10 mM 0.1032 mL 0.5159 mL 1.0318 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Iain J Clarke, et al. Potent action of RFamide-related peptide-3 on pituitary gonadotropes indicative of a hypophysiotropic role in the negative regulation of gonadotropin secretion. Endocrinology

RFRP-1(human)

RFRP-1(human); 纯度: 99.32%

RFRP-1(human) 是促性腺激素抑制激素 (GnIH) 的同系物。RFRP-1(human) 靶向人类促性腺激素释放激素 (GnRH) 神经元和促性腺激素,并有效抑制促性腺激素。RFRP-1(human) 是一种有效的神经肽 FF (NPFF) 受体激动剂,对 NPFF2NPFF1 的 EC50 分别为 0.0011 nM 和 29 nM。

RFRP-1(human)amp;;

RFRP-1(human) Chemical Structure

CAS No. : 311309-25-8

规格 价格 是否有货 数量
5 mg ¥3100 In-stock
10 mg ¥5500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

RFRP-1(human) 相关产品

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生物活性

RFRP-1(human) is a gonadotropin-inhibitory hormone (GnIH) homolog. RFRP-1(human) targets human gonadotropin-releasing hormone (GnRH) neurons and gonadotropes and potently inhibits gonadotropin. RFRP-1(human) is a potent Neuropeptide FF (NPFF) receptor agonist with EC50s of 0.0011 nM and 29 nM for NPFF2 and NPFF1, respectively[1][2][3].

体内研究
(In Vivo)

RFRP-1(human) rapidly and reversibly decreases shortening and relaxation in isolated mammalian cardiac myocytes in a dose dependent manner[2].
Intravenous injection of RFRP-1(human) in mice decreases heart rate, stroke volume, ejection fraction, and cardiac output[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1428.72

Formula

C67H101N19O14S

CAS 号

311309-25-8

Sequence Shortening

MPHSFANLPLRF-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 100 mg/mL (69.99 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.6999 mL 3.4996 mL 6.9993 mL
5 mM 0.1400 mL 0.6999 mL 1.3999 mL
10 mM 0.0700 mL 0.3500 mL 0.6999 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Takayoshi Ubuka, et al. Identification of human GnIH homologs, RFRP-1 and RFRP-3, and the cognate receptor, GPR147 in the human hypothalamic pituitary axis. PLoS One. 2009 Dec 22;4(12):e8400.

    [2]. R Nichols, et al. Human RFamide-related peptide-1 diminishes cellular and integrated cardiac contractile performance. Peptides. 2010 Nov;31(11):2067-74.

    [3]. C Gouardères, et al. Functional differences between NPFF1 and NPFF2 receptor coupling: high intrinsic activities of RFamide-related peptides on stimulation of [35S]GTPgammaS binding. Neuropharmacology. 2007 Feb;52(2):376-86.