标签归档：sodium

Hyaluronic acid sodium(Synonyms: 透明质酸钠; Sodium hyaluronate)

发表于由

天然产物 糖类和糖苷 Saccharides and Glycosides

Hyaluronic acid sodium;(Synonyms: 透明质酸钠; Sodium hyaluronate)

Hyaluronic acid sodium (Sodium hyaluronate) 是由二糖的重复单元组成的生物聚合物，应用领域广泛。

Hyaluronic acid sodium(Synonyms: 透明质酸钠; Sodium hyaluronate)

Hyaluronic acid sodium Chemical Structure

CAS No. : 9067-32-7

规格 价格 是否有货 数量
50 mg ￥500 In-stock
100 mg ￥700 In-stock
200 mg ￥900 In-stock
500 mg ￥1500 In-stock
1 g ￥1900 In-stock
5 g ; 询价 ;
10 g ; 询价 ;

* Please select Quantity before adding items.

Hyaluronic acid sodium 相关产品

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  • Natural Product Library Plus
  • Drug Repurposing Compound Library Plus
  • FDA-Approved Drug Library Plus
  • Bioactive Compound Library Plus

生物活性

Hyaluronic acid sodium (Sodium hyaluronate) is a biopolymer composed of repeating units of disaccharides with various applications.

体外研究
(In Vitro)

Hyaluronic acid (HA) is widely used in aesthetic medicine due to its binding ability with a large number of water molecules. It improves tissue hydration and their resistance to mechanical damage. HA plays an important role in wound healing, ovulation, fertilization, signal transduction, and tumor physiology. HA is used in joint diseases such as osteoarthritis or rheumatoid arthritis. HA of a high molecular mass reduces the chemotaxis and migration of inflammatory cells which acts as a good barrier to the inflammatory process and protects against the effects of free radicals. HA is used in ophthalmology due to its lubricating properties for the corneal endothelium, and improves tissue hydration and cellular resistance to mechanical damage in aesthetic dermatology, and has marginal adverse effects. Several trials indicate its role in tumor markers, liver diseases, and in pharmaceuticals[1]. Hyaluronan plays an important role in cancer growth and metastasis. HA and HA fragment-tumor cell interaction could activate the downstream signaling pathways, promoting cell proliferation, adhesion, migration and invasion, and inducing angiogenesis, lymphangiogenesis, epithelial-mesenchymal transition, stem cell-like property, and chemoradioresistance in digestive cancers[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

The impact of applied intra-articular HA has been proven in many studies in animals. Studies on HA have shown that it promotes the synthesis of cartilage matrix, prevents its degradation, reduces inflammation, stimulates the synthesis of endogenous HA, and improves the resilience and moisture of cartilage[1]. High molecular size HA preparations, applied topically, promote healing of fresh skin wounds. They also promote the healing of venous leg ulcers and are useful in the management of chronic wounds[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

分子量

1500 (kDa)

CAS 号

9067-32-7
中文名称

透明质酸钠；氯化钠透明质酸；玻璃酸钠；糖醛酸钠
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 3.33 mg/mL (Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)
参考文献

  • [1]. Salwowska NM, et al. Physiochemical properties and application of hyaluronic acid: a systematic review. J Cosmet Dermatol. 2016 Dec;15(4):520-526.

    [2]. Wu RL, et al. Hyaluronic acid in digestive cancers. J Cancer Res Clin Oncol. 2017 Jan;143(1):1-16.

    [3]. Kogan G, et al. Hyaluronic acid: a natural biopolymer with a broad range of biomedical and industrial applications. Biotechnol Lett. 2007 Jan;29(1):17-25.

5-BrUTP sodium salt(Synonyms: 5-Bromouridine 5′-triphosphate sodium salt)

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生化分析试剂 Biochemical Assay Reagents
5-BrUTP sodium salt;(Synonyms: 5-Bromouridine 5′-triphosphate sodium salt) 纯度: ge;98.0%

5-BrUTP sodium salt 可以用来标记 RNA 以测量转录。

5-BrUTP sodium salt(Synonyms: 5-Bromouridine 5

5-BrUTP sodium salt Chemical Structure

CAS No. : 161848-60-8

规格 价格 是否有货 数量
5 mg ￥1900 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

5-BrUTP sodium salt 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

5-BrUTP sodium salt can be used to label RNA to measure the transcription.

Formula

C9H14BrN2O15P3.xNa
CAS 号

161848-60-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 33.33 mg/mL (Need ultrasonic)
参考文献

  • [1]. Bui HT, et al. Essential role of paternal chromatin in the regulation of transcriptional activity during mouse preimplantation development. Reproduction. 2011 Jan;141(1):67-77.

Sodium molybdate(Synonyms: 钼酸钠; Molybdate disodium)

发表于由

生化分析试剂 Biochemical Assay Reagents
Sodium molybdate;(Synonyms: 钼酸钠; Molybdate disodium)

Sodium molybdate (Molybdate disodium) 是钼酸的有用来源。常用的为钼酸钠二水合物。

Sodium molybdate(Synonyms: 钼酸钠; Molybdate disodium)

Sodium molybdate Chemical Structure

CAS No. : 7631-95-0

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ￥500 In-stock
500 mg ￥400 In-stock
5 g ￥500 In-stock
10 g ; 询价 ;
50 g ; 询价 ;

* Please select Quantity before adding items.

Sodium molybdate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Orally Active Compound Library

生物活性

Sodium molybdate (Molybdate disodium) is a useful source of molybdate. It is often found as Sodium molybdate dihydrate. In murine models, Sodium molybdate dihydrate inactivated both the active and inactive form of the gluco corticoid receptor complex. With the use of nitrites, molybdate salts have been shown to reduce the emission of hydrogen sulfide from swine manure.

分子量

205.94

Formula

MoNa2O4
CAS 号

7631-95-0
中文名称

钼酸钠
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;

H2O : ≥ 100 mg/mL (485.58 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.8558 mL 24.2789 mL 48.5578 mL
5 mM 0.9712 mL 4.8558 mL 9.7116 mL
10 mM 0.4856 mL 2.4279 mL 4.8558 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

Resorufin sodium salt(Synonyms: 试卤灵钠盐 NSC 12097 sodium salt)

发表于由

Resorufin sodium salt;(Synonyms: 试卤灵钠盐; NSC 12097 sodium salt) 纯度: 96.04%

Resorufin sodium salt (NSC 12097 sodium salt) 是一种高荧光的粉红色染料，用于检测 ROS/RNS 和第二种分析物。

Resorufin sodium saltamp;;(Synonyms: 试卤灵钠盐; NSC 12097 sodium salt)

Resorufin sodium salt Chemical Structure

CAS No. : 34994-50-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥880 In-stock
100 mg ￥800 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Resorufin sodium salt 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Resorufin sodium salt (NSC 12097 sodium salt) is a highly fluorescent pink dye for the detection of ROS/RNS and a second analyte[1].

分子量

235.17

Formula

C12H6NNaO3
CAS 号

34994-50-8
中文名称

试卤灵钠盐
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (425.22 mM; Need ultrasonic)

DMSO : 62.5 mg/mL (265.77 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.2522 mL 21.2612 mL 42.5224 mL
5 mM 0.8504 mL 4.2522 mL 8.5045 mL
10 mM 0.4252 mL 2.1261 mL 4.2522 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (8.84 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (8.84 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Odyniec ML , et al. ‘AND’-based fluorescence scaffold for the detection of ROS/RNS and a second analyte. Chem Commun (Camb). 2018 Jul 26;54(61):8466-8469.

Tartaric acid disodium dihydrate(Synonyms: Sodium tartrate dibasic dihydrate; Sodium tartrate dihydrate)

发表于由

生化分析试剂 Biochemical Assay Reagents
Tartaric acid disodium dihydrate;(Synonyms: Sodium tartrate dibasic dihydrate; Sodium tartrate dihydrate) 纯度: ge;98.0%

酒石酸钠二元二水合物是一种钠盐, 在分子生物学和细胞培养中用作缓冲液, 是酸性磷酸酶抑制剂, 增加秋水仙碱结合tubulin1率。

Tartaric acid disodium dihydrate(Synonyms: Sodium tartrate dibasic dihydrate; Sodium tartrate dihydrate)

Tartaric acid disodium dihydrate Chemical Structure

CAS No. : 6106-24-7

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in Water ￥550 In-stock
500 mg ￥500 In-stock
5 g ￥600 In-stock
10 g ; 询价 ;
50 g ; 询价 ;

* Please select Quantity before adding items.

Tartaric acid disodium dihydrate 相关产品

bull;相关化合物库:

  • Natural Product Like Compound Library
  • Bioactive Compound Library Plus
  • Metabolism/Protease Compound Library
  • Anti-Cancer Compound Library
  • FDA Approved amp; Pharmacopeial Drug Library
  • Gut Microbial Metabolite Library
  • Phosphatase Inhibitor Library
  • Microbial Metabolite Library
  • Food Additive Library
  • Food-Sourced Compound Library
  • Inactive Ingredient Library

生物活性

Tartaric acid disodium dihydrate is a Acid phosphatase inhibitor, is a sodium salt used in buffers for molecular biology and cell culture applications. Increases the rate of colchicine binding to tubulin1.

分子量

232.10

Formula

C4H10Na2O8
CAS 号

6106-24-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (430.85 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.3085 mL 21.5424 mL 43.0849 mL
5 mM 0.8617 mL 4.3085 mL 8.6170 mL
10 mM 0.4308 mL 2.1542 mL 4.3085 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

Sodium carboxymethyl cellulose (MW 250000)(Synonyms: CMC-Na (MW 250000))

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生化分析试剂 Biochemical Assay Reagents
Sodium carboxymethyl cellulose (MW 250000);(Synonyms: CMC-Na (MW 250000))

Sodium carboxymethyl cellulose (CMC-Na) (MW 250000) 是羧甲基纤维素的钠盐，经常用作粘稠剂，糊剂和阻隔剂。

Sodium carboxymethyl cellulose (MW 250000)(Synonyms: CMC-Na (MW 250000))

Sodium carboxymethyl cellulose (MW 250000) Chemical Structure

CAS No. : 9004-32-4

规格 价格 是否有货 数量
100 mg ￥500 In-stock
500 mg ￥1000 In-stock
1 g ￥1400 In-stock
5 g ; 询价 ;
10 g ; 询价 ;

* Please select Quantity before adding items.

生物活性

Sodium carboxymethyl cellulose (CMC-Na) (MW 250000) is the sodium salt of cellulose arboxymethyl and frequently used as viscous agent, paste and barrier agent.

分子量

250000(Average)

CAS 号

9004-32-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
参考文献

  • [1]. Ahmed A Oun, et al. Effect of oxidized chitin nanocrystals isolated by ammonium persulfate method on the properties of carboxymethyl cellulose-based films. Carbohydr Polym. 2017 Nov 1;175:712-720.

Luminol sodium salt(Synonyms: 鲁米诺钠盐)

发表于由

Luminol sodium salt;(Synonyms: 鲁米诺钠盐)

Luminol sodium salt 是一种化学发光物质，pKa 值为 6.74 和 15.1。Luminol sodium salt 最适荧光波长为 425 nm。Luminol sodium salt 常用作法医检测血迹的诊断工具，应用于刑事侦查、生物工程、化学示踪等领域。

Luminol sodium saltamp;;(Synonyms: 鲁米诺钠盐)

Luminol sodium salt Chemical Structure

CAS No. : 20666-12-0

规格 价格 是否有货 数量
500 mg ￥500 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

生物活性

Luminol sodium salt is a chemical that exhibits chemiluminescence with pKa values of 6.74 and 15.1. Luminol sodium salt exhibits chemiluminescence (CL) at 425 nm λmax. Luminol sodium salt is commonly used in forensics as a diagnostic tool for the detection of blood stains[1].

体外研究
(In Vitro)

Luminol concentration is an important factor that affects Chemiluminescence (CL) intensity. The intensity of Luminol chemiluminescence (LCL) does not depend on the concentration of Luminol alone, but also on other factors like concentration of oxidizing agents, enzymes, and pH. Maximum recorded CL intensity was at a Luminol concentration of 0.3 mM. CL intensity increased linearly with increasing concentrations of Luminol in the range of 0.01 to 0.3 mM. However, further increases of Luminol concentration caused a decrease in CL intensity[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

199.14

Formula

C8H6N3NaO2
CAS 号

20666-12-0
中文名称

鲁米诺钠盐
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
参考文献

  • [1]. Michael Mayer, et al. Shedding Light on the Diversity of Surfactant Interactions with Luminol Electrochemiluminescence for Bioanalysis. Anal Chem. 2019 Oct 15;91(20):13080-13087.

MES sodium salt(Synonyms: 2-Morpholinoethanesulphonic acid sodium salt)

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生化分析试剂 Biochemical Assay Reagents
MES sodium salt;(Synonyms: 2-Morpholinoethanesulphonic acid sodium salt) 纯度: ge;95.0%

MES (2-Morpholinoethanesulphonic acid) sodium salt 是一种两性离子缓冲液，在 pH 值 5.5-7.7 范围内有效。MES sodium salt 作为一种 Good’s 缓冲液，广泛应用于植物培养基、试剂溶液和生理实验中调节 pH 值。

MES sodium salt(Synonyms: 2-Morpholinoethanesulphonic acid sodium salt)

MES sodium salt Chemical Structure

CAS No. : 71119-23-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥610 In-stock
500 mg ￥550 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

MES sodium salt 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

MES (2-Morpholinoethanesulphonic acid) sodium salt, a zwitterionic buffer, is effective in the pH range of 5.5-7.7. MES sodium salt, as one of the Good’s buffers, is broadly used to regulate pH value for plants culture medium, reagent solution, and physiological experiments[1][2].

分子量

217.22

Formula

C6H12NNaO4S
CAS 号

71119-23-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 25 mg/mL (115.09 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.6036 mL 23.0181 mL 46.0363 mL
5 mM 0.9207 mL 4.6036 mL 9.2073 mL
10 mM 0.4604 mL 2.3018 mL 4.6036 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (11.51 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (11.51 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (11.51 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Kagenishi T, et al. MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex. Front Plant Sci. 2016;7:79. Published 2016 Feb 18.

    [2]. Mash HE, et al. Complexation of copper by zwitterionic aminosulfonic (good) buffers. Anal Chem. 2003;75(3):671-677.

Sulforhodamine B sodium salt(Synonyms: Acid Red 52 Kiton Red 620)

发表于由

Sulforhodamine B sodium salt;(Synonyms: Acid Red 52; Kiton Red 620)

Sulforhodamine B sodium salt 是一种荧光染料，可用于定量测定培养细胞的细胞蛋白量。

Sulforhodamine B sodium saltamp;;(Synonyms: Acid Red 52; Kiton Red 620)

Sulforhodamine B sodium salt Chemical Structure

CAS No. : 3520-42-1

规格 价格 是否有货
10;mM;*;1 mL in DMSO ￥550 询问价格 货期
100 mg ￥500 询问价格 货期
1 g ￥700 询问价格 货期

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生物活性

Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.

体外研究
(In Vitro)

Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

580.65

Formula

C27H29N2NaO7S2
CAS 号

3520-42-1
中文名称

磺酰罗丹明 B；柴林红 B；奇通红 S；酸性桃红 B；酸性红 52；二甲苯红 B；酸性玫瑰红 B；磺化罗丹明 B；硫罗丹明 B；食品红 106
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 50 mg/mL (86.11 mM; Need ultrasonic)

H2O : 20 mg/mL (34.44 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7222 mL 8.6110 mL 17.2221 mL
5 mM 0.3444 mL 1.7222 mL 3.4444 mL
10 mM 0.1722 mL 0.8611 mL 1.7222 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.
Cell Assay
[1]

Gently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.

Rose Bengal sodium(Synonyms: 酸性红 94)

发表于由

Rose Bengal sodium;(Synonyms: 酸性红 94) 纯度: 99.90%

Rose Bengal sodium，一种合成的荧光素衍生物，是一种深红色的染料，主要成分是 4,5,6,7-tetrachloro-2,4,5,7-tetraiodo 荧光素。Rose Bengal sodium 被广泛用作眼科诊断剂，可以检测眼表干燥或受损的细胞，并具有抗病毒活性。

Rose Bengal sodiumamp;;(Synonyms: 酸性红 94)

Rose Bengal sodium Chemical Structure

CAS No. : 632-69-9

规格 价格 是否有货 数量
10 mg ￥500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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Rose Bengal sodium 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Rose Bengal sodium, a synthetic fluorescein derivative, and is a crimson-coloured dye with the principal component being 4,5,6,7-tetrachloro-2,4,5,7-tetraiodo fluorescein. Rose Bengal sodium has been widely used as an ophthalmic diagnostic agents, and can detect desiccated or damaged cells on the ocular surface. Rose Bengal sodium exhibits antiviral activities[1][2].

分子量

1017.64

Formula

C20H2Cl4I4Na2O5
CAS 号

632-69-9
中文名称

酸性红 94；孟加拉玫瑰红；虎红钠盐；四氯四碘荧光素二钠
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;

H2O : 31.25 mg/mL (30.71 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9827 mL 4.9133 mL 9.8267 mL
5 mM 0.1965 mL 0.9827 mL 1.9653 mL
10 mM 0.0983 mL 0.4913 mL 0.9827 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Doughty MJ, et, al. Fluorescence characteristics of sodium fluorescein-rose bengal ophthalmic solution mixtures. Cont Lens Anterior Eye. 2014 Oct; 37(5): 358-62.

    [2]. Roat MI, et, al. The antiviral effects of rose bengal and fluorescein. Arch Ophthalmol. 1987 Oct; 105(10): 1415-7.