BW-180C(Synonyms: [D-Ala2, D-Leu5]-Enkephalin DADLE)

BW-180C;(Synonyms: [D-Ala2, D-Leu5]-Enkephalin; DADLE) 纯度: 99.65%

BW-180C ([D-Ala2, D-Leu5]-Enkephalin) 是水溶性的多肽类阿片受体 (Opioid Receptor) 激动剂。

BW-180Camp;;(Synonyms: [D-Ala2, D-Leu5]-Enkephalin;  DADLE)

BW-180C Chemical Structure

CAS No. : 63631-40-3

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥965 In-stock
5 mg ¥770 In-stock
10 mg ¥1200 In-stock
25 mg ¥2400 In-stock
50 mg ¥3900 In-stock
100 mg ¥5900 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

BW-180C ([D-Ala2, D-Leu5]-Enkephalin) is a water soluble Opioid Receptor peptide agonist.

IC50 Target

Opioid Receptor[1]

体外研究
(In Vitro)

BW-180C ([D-Ala2, D-Leu5]-Enkephalin) significantly inhibits cellular transcription in SH-SY5Y cells without causing cell injury. Following recovery for 72 h without BW-180C in primary neurons, the transcriptional activity fully resumes[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

BW-180C ([D-Ala2, D-Leu5]-Enkephalin) protects against I-R injury in hepatocytes, but not in the sinusoidal endothelial cells of the liver in rats. GPT levels are significantly lower in the BW-180C group as compared to those of the Control group, but the serum levels of HA are not different between the two groups. The concentrations of MDA of the liver tissue are significantly lower in the BW-180C group than in the Control group[2]. BW-180C (DADLE)-induced analgesia is mediated by the stimulation of both mu- and delta-opioid receptors[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

569.65

Formula

C29H39N5O7

CAS 号

63631-40-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : ≥ 300 mg/mL (526.64 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7555 mL 8.7773 mL 17.5546 mL
5 mM 0.3511 mL 1.7555 mL 3.5109 mL
10 mM 0.1755 mL 0.8777 mL 1.7555 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Tian J, et al. [D-Ala2, D-Leu5] encephalin (DADLE) reversibly inhibits cellular transcription in neurons without causing cell injury. Brain Res. 2014 May 27;1565:1-7.

    [2]. Yamanouchi K, et al. [D-Ala2, D-Leu5] enkephalin (DADLE) protects liver against ischemia-reperfusion injury in the rat. J Surg Res. 2003 Sep;114(1):72-7.

    [3]. Suh HH, et al. Different types of opioid receptors mediating analgesia induced by morphine, DAMGO, DPDPE, DADLE and beta-endorphin in mice. Naunyn Schmiedebergs Arch Pharmacol. 1990 Jul;342(1):67-71.

Cell Assay
[1]

Primary cortical neurons are plated in 96-well plates and cultured with 100 nM BW-180C for 72 h. 5 mg/mL MTT in an amount equal to 10% of the culture volume is added to each well 4 h before the end of treatment. After a 4-h incubation, the media is removed, and MTT solvent in amount equal to original culture volume is added to each well to dissolve the crystals. The formation of formazan is analyzed within one hour after adding MTT solvent, by measuring the absorbance at a wavelength of 570 nm and a reference wavelength of 690 nm by using a microplate reader. The values in absorbance are normalized to the PBS-treated control to calculate the fold change[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2][3]

Rats[2]

After administration of BW-180C (BW-180C group) or normal saline as a vehicle (Control group), partial hepatic ischemia is induced by occluding the vessels supplying 92% of the liver for 45 min, followed by declamping the vessels and resection of the non-ischemic lobe. After 120 min of reperfusion, serum glutamic-pyruvic transaminase (GPT), hyaluronic acid (HA) levels, and concentrations of malondialdehyde (MDA) of the liver tissue are measured. Additionally, bile output from the ischemic lobes is measured after reperfusion[2].

Mice[3]

A single i.c.v, injection of opioid agonist alone or agonist in combination with antagonist. Injection volume for i.c.v, injection is 5 μL. The dose-response relationships are established by injecting i.c.v, with different doses of morphine sulfate, DAMGO, DPDPE, BW-180C, or p-endorphin in the presence or absence of a fixed dose of CTOP (0.025 or 0.05 μg), ICI 174864 (5 μg), or ICI 154129 (5 μg). The agonists and antagonists are mixed, and given as a single i.c.v, injection[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Tian J, et al. [D-Ala2, D-Leu5] encephalin (DADLE) reversibly inhibits cellular transcription in neurons without causing cell injury. Brain Res. 2014 May 27;1565:1-7.

    [2]. Yamanouchi K, et al. [D-Ala2, D-Leu5] enkephalin (DADLE) protects liver against ischemia-reperfusion injury in the rat. J Surg Res. 2003 Sep;114(1):72-7.

    [3]. Suh HH, et al. Different types of opioid receptors mediating analgesia induced by morphine, DAMGO, DPDPE, DADLE and beta-endorphin in mice. Naunyn Schmiedebergs Arch Pharmacol. 1990 Jul;342(1):67-71.

D-Ala-Lys-AMCA

D-Ala-Lys-AMCA;

D-Ala-Lys-AMCA 是一种已知的质子偶联寡肽转运蛋白 1 (PEPT1) 底物,可发出蓝色荧光。D-Ala-Lys-AMCA 可能被转运到肝癌细胞和 Caco-2 细胞中。

D-Ala-Lys-AMCAamp;;

D-Ala-Lys-AMCA Chemical Structure

CAS No. : 375822-19-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

D-Ala-Lys-AMCA 的其他形式现货产品:

D-Ala-Lys-AMCA hydrochloride

生物活性

D-Ala-Lys-AMCA is a known proton-coupled oligopeptide transporter 1 (PEPT1) substrate that emits blue fluorescence. D-Ala-Lys-AMCA may be transported into liver cancer cells and Caco-2 cells based on fluorescence analysis[1].

分子量

432.47

Formula

C21H28N4O6

CAS 号

375822-19-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Gong Y, et al. Specific expression of proton-coupled oligopeptide transporter 1 in primary hepatocarcinoma-anovel strategy for tumor-targeted therapy. Oncol Lett. 2017 Oct;14(4):4158-4166.

[D-Ala2]leucine-enkephalin

[D-Ala2]leucine-enkephalin; 纯度: 99.19%

[D-Ala2]leucine-enkephalin 是一种 δ 阿片类 (delta opioid) 激动剂,是一种能长效抗降解的亮氨酸脑非肽。

[D-Ala2]leucine-enkephalinamp;;

[D-Ala2]leucine-enkephalin Chemical Structure

CAS No. : 64963-01-5

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ¥752 In-stock
5 mg ¥600 In-stock
10 mg ¥900 In-stock
50 mg ¥3500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

[D-Ala2]leucine-enkephalin 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Neuronal Signaling Compound Library
  • Anti-Cancer Compound Library
  • Peptidomimetic Library
  • Neurotransmitter Receptor Compound Library
  • Peptide Library

生物活性

[D-Ala2]leucine-enkephalin, a delta opioid agonist, is a degradation resistant long-acting Leu-enkephalin.

分子量

569.65

Formula

C29H39N5O7

CAS 号

64963-01-5

Sequence

Tyr-{d-Ala}-Gly-Phe-Leu

Sequence Shortening

Y-{d-Ala}-GFL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 50 mg/mL (87.77 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7555 mL 8.7773 mL 17.5546 mL
5 mM 0.3511 mL 1.7555 mL 3.5109 mL
10 mM 0.1755 mL 0.8777 mL 1.7555 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

Mca-Ala-Pro-Lys(Dnp)-OH

Mca-Ala-Pro-Lys(Dnp)-OH; 纯度: 98.99%

Mca-Ala-Pro-Lys(Dnp)-OH 是特异的 ACE2 猝灭荧光底物,可用于检测尿、心、肺 ACE2 活性。

Mca-Ala-Pro-Lys(Dnp)-OHamp;;

Mca-Ala-Pro-Lys(Dnp)-OH Chemical Structure

CAS No. : 305336-82-7

规格 价格 是否有货 数量
10 mg ¥2200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Mca-Ala-Pro-Lys(Dnp)-OH 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Mca-Ala-Pro-Lys(Dnp)-OH, a specific ACE2 quenched fluorogenic substrate, can be used to detect ACE2 activity, such as urinary, heart and lung[1][2].

体外研究
(In Vitro)

The activity of ACE2 enzyme detected by: the fluorescent peptide substrate Mca-AlaPro-Lys (Dnp)-OH is hydrolyzed by ACE2 and its product is detected by the active fluorescence resonance energy transfer method[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

696.66

Formula

C32H36N6O12

CAS 号

305336-82-7

Sequence Shortening

Mca-AP{K(Dnp)}

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 50 mg/mL (71.77 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4354 mL 7.1771 mL 14.3542 mL
5 mM 0.2871 mL 1.4354 mL 2.8708 mL
10 mM 0.1435 mL 0.7177 mL 1.4354 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.59 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.59 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Liu J, Ji H, et al. Sex differences in renal angiotensin converting enzyme 2 (ACE2) activity are 17β-oestradiol-dependent and sex chromosome-independent. Biol Sex Differ. 2010;1(1):6. Published 2010 Nov 5.

    [2]. Liang Y, et al. Urinary angiotensin converting enzyme 2 increases in patients with type 2 diabetic mellitus. Kidney Blood Press Res. 2015;40(2):101-110.

D-Ala-Lys-AMCA hydrochloride

D-Ala-Lys-AMCA hydrochloride; 纯度: 99.54%

D-Ala-Lys-AMCA hydrochloride 是一种已知的质子偶联寡肽转运蛋白 1 (PEPT1) 底物,可发出蓝色荧光。D-Ala-Lys-AMCA hydrochloride 可能被转运到肝癌细胞和 Caco-2 细胞中。

D-Ala-Lys-AMCA hydrochlorideamp;;

D-Ala-Lys-AMCA hydrochloride Chemical Structure

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥3920 In-stock
1 mg ¥2000 In-stock
5 mg ¥3800 In-stock
10 mg ¥6500 In-stock
25 mg ¥13000 In-stock
50 mg ¥22000 In-stock
100 mg ¥36000 询价
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

D-Ala-Lys-AMCA hydrochloride 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Cancer Compound Library
  • Targeted Diversity Library
  • Anti-Liver Cancer Compound Library
  • Peptide Library

生物活性

D-Ala-Lys-AMCA hydrochloride is a known proton-coupled oligopeptide transporter 1 (PEPT1) substrate that emits blue fluorescence. D-Ala-Lys-AMCA hydrochloride may be transported into liver cancer cells and Caco-2 cells based on fluorescence analysis[1].

分子量

468.93

Formula

C21H29ClN4O6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

DMSO : 250 mg/mL (533.13 mM; Need ultrasonic)

H2O : 100 mg/mL (213.25 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1325 mL 10.6626 mL 21.3251 mL
5 mM 0.4265 mL 2.1325 mL 4.2650 mL
10 mM 0.2133 mL 1.0663 mL 2.1325 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.44 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.44 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.44 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.44 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Gong Y, et al. Specific expression of proton-coupled oligopeptide transporter 1 in primary hepatocarcinoma-anovel strategy for tumor-targeted therapy. Oncol Lett. 2017 Oct;14(4):4158-4166.

D-Ala-Lys-AMCA TFA

D-Ala-Lys-AMCA TFA;

D-Ala-Lys-AMCA TFA 是一种已知的质子偶联寡肽转运蛋白 1 ( PEPT1) 底物,可发出蓝色荧光。D-Ala-Lys-AMCA TFA 可能被转运到肝癌细胞和 Caco-2 细胞中。

D-Ala-Lys-AMCA TFAamp;;

D-Ala-Lys-AMCA TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

D-Ala-Lys-AMCA TFA 的其他形式现货产品:

D-Ala-Lys-AMCA hydrochloride

生物活性

D-Ala-Lys-AMCA TFA is a known proton-coupled oligopeptide transporter 1 (PEPT1) substrate that emits blue fluorescence. D-Ala-Lys-AMCA TFA may be transported into liver cancer cells and Caco-2 cells based on fluorescence analysis[1].

分子量

546.49

Formula

C23H29F3N4O8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Gong Y, et al. Specific expression of proton-coupled oligopeptide transporter 1 in primary hepatocarcinoma-anovel strategy for tumor-targeted therapy. Oncol Lett. 2017 Oct;14(4):4158-4166.

[Ala17]-MCH

[Ala17]-MCH; 纯度: 98.19%

[Ala17]-MCH 是一种 MCH (HY-P1525A) 的类似物,是 MCHR1 的配体 (Ki=0.16 nM) 选择性 优于对 MCHR2 的 (Ki=34 nM)。Eu3+螯合标记 [Ala17]-MCH 对 MCHR1 (Kd=0.37 nM) 亲和性高,而对 MCHR2 的亲和性低。

[Ala17]-MCHamp;;

[Ala17]-MCH Chemical Structure

CAS No. : 359784-84-2

规格 价格 是否有货 数量
1 mg ¥2200 In-stock
5 mg ¥8800 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

[Ala17]-MCH 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

[Ala17]-MCH, a MCH analogue (HY-P1525A), is a selective ligand for MCHR1 (Ki=0.16 nM) over MCHR2 (Ki=34 nM). [Eu3+ chelate-labeled [Ala17]-MCH shows high affnity for MCHR1 (Kd=0.37 nM) while has little demonstrable binding affnity for MCHR2[1].

IC50 Target

Ki: 0.16 nM (MCHR1)
Ki: 34 nM (MCHR2)[1]

分子量

2271.71

Formula

C97H155N29O26S4

CAS 号

359784-84-2

Sequence Shortening

DFDMLRCMLGRVYRPCAQV (Disulfide bridge:Cys7-Cys16)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 25 mg/mL (11.00 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.4402 mL 2.2010 mL 4.4020 mL
5 mM 0.0880 mL 0.4402 mL 0.8804 mL
10 mM 0.0440 mL 0.2201 mL 0.4402 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (1.10 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.10 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (1.10 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.10 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (1.10 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (1.10 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Xiaoying Gao, et al. Europium-labeled melanin-concentrating hormone analogues: ligands for measuring binding to melanin-concentrating hormone receptors 1 and 2. Anal Biochem. 2004 May 15;328(2):187-95.

Fmoc-Ala-Glu-Asn-Lys-NH2

Fmoc-Ala-Glu-Asn-Lys-NH2; 纯度: 98.04%

Fmoc-Ala-Glu-Asn-Lys-NH2 是一种选择性天冬酰胺内肽酶 AEP 抑制肽,可抑制淀粉样前体蛋白 APP 裂解。AEP是一种 pH 值控制的半胱氨酸蛋白酶,在衰老过程中被激活并介导 APP 蛋白水解过程。

Fmoc-Ala-Glu-Asn-Lys-NH2amp;;

Fmoc-Ala-Glu-Asn-Lys-NH2 Chemical Structure

CAS No. : 220701-06-4

规格 价格 是否有货 数量
5 mg ¥1350 In-stock
10 mg ¥2350 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Fmoc-Ala-Glu-Asn-Lys-NH2 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Neuronal Signaling Compound Library
  • Anti-Alzheimer’s Disease Compound Library
  • Peptide Library

生物活性

Fmoc-Ala-Glu-Asn-Lys-NH2 is a selective asparagine endopeptidase (AEP) inhibitor peptide and suppresses amyloid precursor protein (APP) cleavage. AEP, a pH-controlled cysteine proteinase, is activated during ageing and mediates APP proteolytic processing[1].

体外研究
(In Vitro)

Fmoc-Ala-Glu-Asn-Lys-NH2 antagonizes APP processing by AEP, whereas other small molecular inhibitors and inactive peptide AEQK were without effect[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

681.74

Formula

C33H43N7O9

CAS 号

220701-06-4

Sequence Shortening

{Fmoc}-AENK-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light, stored under nitrogen

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (146.68 mM; ultrasonic and warming and heat to 80°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4668 mL 7.3342 mL 14.6683 mL
5 mM 0.2934 mL 1.4668 mL 2.9337 mL
10 mM 0.1467 mL 0.7334 mL 1.4668 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Zhang Z, et al. Delta-secretase cleaves amyloid precursor protein and regulates the pathogenesis in Alzheimer’s disease. Nat Commun. 2015;6:8762. Published 2015 Nov 9.

[Sar1,Val5,Ala8]-Angiotensin II 品牌:Peptide


[Sar1,Val5,Ala8]-Angiotensin II

品牌:Peptide
CAS No.:34273-10-4
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

334-40713

25mg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

[D-Ala2,Met5]-Enkephalin 品牌:Peptide


[D-Ala2,Met5]-Enkephalin

品牌:Peptide
CAS No.:
储存条件:-20℃
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

337-41163

25mg 咨询


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。