标签归档：human

Endothelin-3, human, mouse, rabbit, rat TFA(Synonyms: Endothelin 3 (Rat,Human) (TFA))

发表于由

Endothelin-3, human, mouse, rabbit, rat TFA;(Synonyms: Endothelin 3 (Rat,Human) (TFA)) 纯度: 99.66%

Endothelin-3, human, mouse, rabbit, rat TFA 是由21个氨基酸组成的血管活性肽，可与G蛋白连接的跨膜受体，ET-RA 和ET-RB 结合。

Endothelin-3, human, mouse, rabbit, rat TFA (Synonyms: Endothelin 3 (Rat,Human) (TFA))

Endothelin-3, human, mouse, rabbit, rat TFA Chemical Structure

规格 价格 是否有货 数量
500 μg ￥1800 In-stock
1 mg ￥2800 In-stock
5 mg ￥9800 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Endothelin-3, human, mouse, rabbit, rat TFA 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Endothelin-3, human, mouse, rabbit, rat TFA is a 21-amino acid vasoactive peptide that binds to G-protein-linked transmembrane receptors, ET-RA and ET-RB.

IC50 & Target

ET-RA, ET-RB[1]
分子量

2757.07

Formula

C123H169F3N26O35S4
Sequence

Cys-Thr-Cys-Phe-Thr-Tyr-Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp(Disulfide bridge: Cys1-Cys15, Cys3-Cys11)
Sequence Shortening

CTCFTYKDKECVYYCHLDIIW(Disulfide bridge: Cys1-Cys15, Cys3-Cys11)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (<50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Skovsted GF, et al. Endothelin-1 and Endothelin-3 Regulate Endothelin Receptor Expression in Rat Coronary Arteries. Basic Clin Pharmacol Toxicol. 2015 Nov;117(5):297-305.

Orexin A (human, rat, mouse)

发表于由

Orexin A (human, rat, mouse);

Orexin A human, rat, mouse 是一种 33 氨基酸兴奋性神经肽，参与多种中枢和外周过程。Orexin A human, rat, mouse 是一种特异性、高亲和力的 G 蛋白偶联受体 OX1R 激动剂，在调节摄食行为中起重要作用。Orexin A human, rat, mouse 是一种有效的抗伤害和抗痛觉过敏的药物。

Orexin A (human, rat, mouse)&nbsp;

Orexin A (human, rat, mouse) Chemical Structure

CAS No. : 205640-90-0

规格 价格 是否有货
1 mg ￥4400 询问价格 & 货期
5 mg ￥18000 询问价格 & 货期

* Please select Quantity before adding items.

Orexin A (human, rat, mouse) 的其他形式现货产品:

Orexin A (human, rat, mouse) (TFA)

生物活性

Orexin A human, rat, mouse, a 33 amino acid excitatory neuropeptide, orchestrates diverse central and peripheral processes. Orexin A human, rat, mouse is a specific, high-affinity agonist for G-protein-coupled receptor OX1R. Orexin A human, rat, mouse has a role in the regulation of feeding behavior. Orexin A human, rat, mouse is an effective anti-nociceptive and anti-hyperalgesic agent in mice and rats[1][2].

体外研究
(In Vitro)

Orexin A human, rat, mouse has high affinity for OX1R, with 38 nM IC50 and 34 nM EC50 values in the the [Ca2﹢]i transient assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Orexin A human, rat, mouse (3-30 mg/kg; i.v.; 5 min pre-test) significantly increases the latency to response at 10 and 30 mg/kg i.v. when given 5 min pre-test from 24.8±2.0 s in vehicle-treated mice to 35.0±3.7 s and 45.7±4.5 s, respectively[2].
Orexin A human, rat, mouse (3, 10 and 30 mg/kg; i.v.) was given immediately before phenylp-quinone (PPQ) and increases the latency to the first PPQ-induced constriction from 357.4±35.2 s in vehicle-treated mice to 500.3±31.2 s at 10 mg/kg and 594.5±5.5 s at 30 mg/kg[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female mice (mouse carrageenan-induced thermal hyperalgesia test)[2]
Dosage: 3, 10 and 30 mg/kg
Administration: i.v.; 5 min pre-test
Result: Significantly increased the latency to response at 10 and 30 mg/kg.

分子量

3561.10

Formula

C152H243N47O44S4
CAS 号

205640-90-0
Sequence

{Glp}-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys-Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-IIE-Leu-Thr-Leu-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
Sequence Shortening

{Glp}-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (<50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Sakurai T, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell. 1998 Feb 20;92(4):573-85.

    [2]. Bingham S, et al. Orexin-A, an hypothalamic peptide with analgesic properties. Pain. 2001 May;92(1-2):81-90.

Apelin-17(human, bovine)

发表于由

Apelin-17(human, bovine); 纯度: 98.86%

Apelin-17(human, bovine) 是一种内源性孤儿 G 蛋白偶联受体 APJ 激动剂。Apelin-17(human, bovine) 与在 HEK 293 细胞中表达的人 APJ 受体结合 (pIC50=9.02)。Apelin-17(human, bovine) 阻断 HIV-1 和 HIV-2 毒株进入表达 APJ 的 NP-2/CD4 细胞。

Apelin-17(human, bovine)amp;;

Apelin-17(human, bovine) Chemical Structure

CAS No. : 217082-57-0

规格 价格 是否有货 数量
1 mg ￥700 In-stock
5 mg ￥2100 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Apelin-17(human, bovine) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Apelin-17(human, bovine) is an endogenous orphan G protein-coupled receptor APJ agonist. Apelin-17(human, bovine) binds to human APJ receptors expressed in HEK 293 cells (pIC50=9.02). Apelin-17(human, bovine) inhibits the entry of some HIV-1 and HIV-2 into the NP2/CD4 cells expressing APJ[1][2][3].

IC50 Target[3]

HIV-1

;

HIV-2

;

分子量

2138.55

Formula

C96H156N34O20S
CAS 号

217082-57-0
Sequence

Lys-Phe-Arg-Arg-Gln-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe
Sequence Shortening

KFRRQRPRLSHKGPMPF
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
参考文献

  • [1]. Tatemoto K, Hosoya M, Habata Y, et al. Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor. Biochem Biophys Res Commun. 1998;251(2):471-476.

    [2]. Medhurst AD, Jennings CA, Robbins MJ, et al. Pharmacological and immunohistochemical characterization of the APJ receptor and its endogenous ligand apelin. J Neurochem. 2003;84(5):1162-1172.

    [3]. Zou MX, et al. Apelin peptides block the entry of human immunodeficiency virus (HIV). FEBS Lett. 2000;473(1):15-18.

GLP-2(1-33)(human)(Synonyms: GLP-2 (human) Glucagon-like peptide 2 (human))

发表于由

GLP-2(1-33)(human);(Synonyms: GLP-2 (human); Glucagon-like peptide 2 (human)) 纯度: 99.18%

GLP-2(1-33) (human) 是一种肠内分泌激素可以与GLP-2 受体结合并且刺激肠上皮细胞生长。

GLP-2(1-33)(human)&nbsp;(Synonyms: GLP-2 (human); Glucagon-like peptide 2 (human))

GLP-2(1-33)(human) Chemical Structure

CAS No. : 223460-79-5

规格 价格 是否有货 数量
500 μg ￥1500 In-stock
1 mg ￥2500 In-stock
5 mg ￥9500 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

GLP-2(1-33)(human) 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

GLP-2(1-33) (human) is an enteroendocrine hormone which can bind to the GLP-2 receptor and stimulate the growth of intestinal epithelium.

IC50 & Target

GLP-2 receptor[1]
体外研究
(In Vitro)

GLP-2-treated group demonstrates a 518±22% increase (P<0.05) in mucosal IGFBP-4 mRNA levels as compare with vehicle-treated controls. Because the mucosal expression of IGFBP-4 transcripts is found to be very low relative to that of the whole intestine, FRIC cultures are used as an in vitro model of the entire intestine. FRIC cultures have previously been established to express a functional GLP-2 receptor (GLP-2R) that displays a cAMP response, as well as enhances IGF-1 mRNA expression and IGF-1 secretion in response to GLP-2 treatment. When incubates with GLP-2 (10−8 M) for 2 hours, IGFBP-4 mRNA expression in the FRIC cultures is also found to be increased, by 40.8±15.2% (P<0.05), compare with vehicle-treated cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

GLP-2 quickly increases apoB48 mass in the TRL fraction of plasma, which is indicative of chylomicron number, and this is blocked by L-NAME. GLP-2 treatment alone increases postprandial TRL-lipids (slope 3.65±0.73×10−3 g/L/min vs 1.63±0.28×10−3 g/L/min, GLP-2 vs control), and this effect is completely mitigated by L-NAME pretreatment (slope 3.67±0.15×10−4 g/L/min). The GLP-2-induced rise in TRL-apoB48 occurres within 30 minutes and precedes the rise in TRL-TG. GLP-2 acutely increases plasma tritium levels (slope, 1.66±0.25×102 dpm/mL/min vs 1.11±0.17×102 dpm/mL/min, GLP-2 vs control)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

3766.19

Formula

C165H254N44O55S
CAS 号

223460-79-5
Sequence

His-Ala-Asp-Gly-Ser-Phe-Ser-Asp-Glu-Met-Asn-Thr-Ile-Leu-Asp-Asn-Leu-Ala-Ala-Arg-Asp-Phe-Ile-Asn-Trp-Leu-Ile-Gln-Thr-Lys-Ile-Thr-Asp
Sequence Shortening

HADGSFSDEMNTILDNLAARDFINWLIQTKITD
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro:;

H2O : 25 mg/mL (6.64 mM; ultrasonic and adjust pH to 9 with NH4OH)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2655 mL 1.3276 mL 2.6552 mL
5 mM 0.0531 mL 0.2655 mL 0.5310 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Kaori Austin, et al. IGF Binding Protein-4 is Required for the Growth Effects of Glucagon-Like Peptide-2 in Murine Intestine. Endocrinology. 2015 Feb; 156(2): 429-436.

    [2]. Hsieh J, et al. Glucagon-Like Peptide 2 (GLP-2) Stimulates Postprandial Chylomicron Production and Postabsorptive Release of Intestinal Triglyceride Storage Pools via Induction of Nitric Oxide Signaling in Male Hamsters and Mice. Endocrinology. 2015 Oct;156(10):3538-47.
Cell Assay
[1]

Fetal rat intestinal cells (FRICs) are collected by enzymatical digestion of whole fetal rat intestines (mixed sex) and cultured overnight in DMEM containing 4.5 g/L glucose, 5% fetal bovine serum, and 40 U/mL penicillin and 40 μg/mL streptomycin in 10 cm plates. Cells are then treated with GLP-2 (10−8 M) or vehicle in low-glucose DMEM with 0.5% FBS for 0.5 to 24 hours[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Hamsters that undergo intraduodenal catheterization (idc) are allowed 7 days of recovery before jugular vein catheterization. For postprandial experiments, conscious fasted (16 h) hamsters receive a 75 mmol/kg iv bolus of L-NAME. After 10 minutes, baseline blood sample is collected (time 0), followed by an oralgastric gavage of 3 μL Ci [9,10-3H(N)] triolein in 200 μL olive oil. At t=10 minutes, F-127 is given ip (1 g/kg) to inhibit peripheral TG-rich lipoprotein (TRL) catabolism. At t=20 minutes, the hamsters receive ip injections of either PBS or 0.25 mg/kg human GLP-2(1-33). Blood (400 μL) is collected every 30 minutes for 2 hours. Blood is centrifuged at 6000 rpm for 10 minutes at 4°C to isolate plasma. Radioactivity is assessed using a liquid scintillation counter[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Kaori Austin, et al. IGF Binding Protein-4 is Required for the Growth Effects of Glucagon-Like Peptide-2 in Murine Intestine. Endocrinology. 2015 Feb; 156(2): 429-436.

    [2]. Hsieh J, et al. Glucagon-Like Peptide 2 (GLP-2) Stimulates Postprandial Chylomicron Production and Postabsorptive Release of Intestinal Triglyceride Storage Pools via Induction of Nitric Oxide Signaling in Male Hamsters and Mice. Endocrinology. 2015 Oct;156(10):3538-47.

Corticotropin-releasing factor (human)(Synonyms: Human CRF Human corticotropin-releasing factor)

发表于由

Corticotropin-releasing factor (human);(Synonyms: Human CRF; Human corticotropin-releasing factor) 纯度: 98.44%

Corticotropin-releasing factor human (Human CRF) 能够刺激垂体前叶的促肾上腺皮质激素的合成和分泌。

Corticotropin-releasing factor (human)&nbsp;(Synonyms: Human CRF; Human corticotropin-releasing factor)

Corticotropin-releasing factor (human) Chemical Structure

CAS No. : 86784-80-7

规格 价格 是否有货 数量
250 μg ￥650 In-stock
500 μg ￥1000 In-stock
1 mg ￥1600 In-stock
5 mg ￥5600 In-stock
10 mg ￥9500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Corticotropin-releasing factor (human) 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Corticotropin-releasing factor human (Human CRF) stimulates the synthesis and secretion of adrenocorticotropin in the anterior pituitary.

体外研究
(In Vitro)

CRF increases excitability of type II dlBNST neurons through activation of the AC-cAMP-PKA pathway, thereby causing pain-induced aversive responses[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

In rats injected with CRF (1 nmol/side), the time spent in the drug-paired compartment during the test session (314±22 s) is significantly shorter than time spent in that compartment during the preconditioning session (520±18 s)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

4757.45

Formula

C208H344N60O63S2
CAS 号

86784-80-7
Sequence

Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2
Sequence Shortening

SEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEII-NH2
中文名称

促肾上腺皮质激素释放因子 (人)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80°C 2 years
-20°C 1 year
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro:;

H2O : 25 mg/mL (5.25 mM; ultrasonic and adjust pH to 2 with HCl)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2102 mL 1.0510 mL 2.1020 mL
5 mM 0.0420 mL 0.2102 mL 0.4204 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Kaneko T et al. Activation of adenylate cyclase-cyclic AMP-protein kinase A signaling by corticotropin-releasing factorwithin the dorsolateral bed nucleus of the stria terminalis is involved in pain-induced aversion. Eur J Neurosci. 2016 Sep 30.

    [2]. Tenk J et al. Acute central effects of corticotropin-releasing factor (CRF) on energy balance: Effects of age and gender. Peptides. 2016 Nov;85:63-72.

Luteinizing hormone (human)(Synonyms: 黄体生成素 (人))

发表于由

Luteinizing hormone (human);(Synonyms: 黄体生成素 (人))

Luteinizing hormone (human) 是垂体 (LH) 产生的一种异二聚体糖蛋白激素，在人类生殖中起关键作用。

Luteinizing hormone (human)amp;;(Synonyms: 黄体生成素 (人))

Luteinizing hormone (human) Chemical Structure

CAS No. : 39341-83-8

规格 价格 是否有货
10 μg ￥7350 询问价格 货期

* Please select Quantity before adding items.

生物活性

Luteinizing hormone (human), a heterodimeric glycoprotein hormone produced by the pituitary gland (LH), plays key roles in human reproduction[1].

IC50 Target

LHR[1]
体外研究
(In Vitro)

Luteinizing hormone (human) binds to luteinizing hormone receptor (LHR) in human and non-human species which are mainly expressed in the ovary and testis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
CAS 号

39341-83-8
中文名称

黄体生成素 (人)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Laura Riccetti, et al. Human Luteinizing Hormone and Chorionic Gonadotropin Display Biased Agonism at the LH and LH/CG Receptors. Sci Rep. 2017 Apr 19;7(1):940.

PD-1（人）ELISA试剂盒 PD-1 (human) ELISA Kit

发表于由

PD-1（人）ELISA试剂盒
PD-1 (human) ELISA Kit

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

PD-1（人）ELISA试剂盒 PD-1 (human) ELISA Kit

PD-1 (human) ELISA Kit

PD-1 (人) ELISA试剂盒



◆规格/处理


产品细节

别名

　CD279；细胞程序性死亡蛋白1

产品规格

　套

性能

应用

　定量ELISA

特异性

　在血清，血浆和细胞培养上清液中检测可溶性的人PD-1（sPD-1）。

交叉反应

　人

数量

　1×96 wells

灵敏度

　1.6 pg/mL

检测范围

　3.125 ~200 pg/mL

样品类型

　细胞培养上清液、血浆、血清

分析类型

　夹心法

检测类型

　比色法

其他产品数据

　UniProt link Q15116:   PD-1 (human)

登记号

　Q15116

运输和处理

运输

　冰袋（BI）

短期储存

　+4°C

长期储存

　+4°C

建议保存方法

　●　标准品配制后，分装好并储存在-20°C。
　●　避免反复冻融。
　●　微孔板和试剂使用前应回温至室温。

使用/稳定性

　有效期为生产后的12个月。请参阅ELISA试剂盒上的失效日期。

◆描述


PD-1（也称为细胞程序性死亡蛋白1；CD279）是CD28/CTLA-4免疫受体家族的I型跨膜蛋白，参与信号和免疫调节。CD28/CTLA-4家族成员可以促进T细胞活性（CD28和ICOS），也可以下调T细胞活性（CTLA-4和PD-1）。PD-1在活化的T细胞、B细胞、骨髓细胞和胸腺细胞中均有表达。PD-1与PD-L1（B7-H1；CD274）或PD-L2（B7-DC；CD273）结合后，可抑制TCR介导的T细胞增殖和生成IL-1、IL-4、IL-10和IFN-γ。另外，PD-1结合后也抑制BCR介导的信号传导。PD-1缺陷型小鼠会有外周耐受性缺陷，会自发发展为自身免疫疾病。可溶性的PD-1（sPD-1）能在活化的免疫细胞中表达，并且可以在血清/血浆中分泌。sPD-1或sPD-L1等可溶性检查点，会参与到正负免疫调节，它们在血清、血浆水平的变化会影响癌症的发展、预测和治疗。不同癌症的患者，所检测到sPD-1或sPD-L1水平会有所升高。PD-1（人）ELISA试剂盒是为检测不同的生物体液中特异性sPD-1（人）而开发的。


PD-1（人）ELISA试剂盒 PD-1 (human) ELISA Kit

标准曲线



※ 本页面产品仅供研究用，研究以外不可使用。

产品列表
产品编号 产品名称 产品规格 产品等级 备注
AG-45B-0015-KI01  PD-1 (human) ELISA Kit
 PD-1 (人) ELISA试剂盒		 96 wells

Tim-4（小鼠）：Fc（人）（重组）（生物素标记） Tim-4 (mouse):Fc (human) (rec.) (Biotin)

发表于由

Tim-4（小鼠）：Fc（人）（重组）（生物素标记）
Tim-4 (mouse):Fc (human) (rec.) (Biotin)

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

Tim-4（小鼠）：Fc（人）（重组）（生物素标记）

参考文献


[1]

A novel affinity-based method for the isolation of highly purified extracellular vesicles:W. Nakai, et al.; Sci. Rep. 6, 33935 (2016)

[2]

High purity isolation and sensitive quantification of extracellular vesicles using affinity to Tim-4: T. Yoshida, et al.; Curr. Prot. Cell Biol. 77, 3.45.1-3.45.18 (2017)

产品列表
产品编号 产品名称 产品规格 产品等级 备注
AG-40B-0180B-C010 Tim-4 (mouse):Fc (human) (rec.) (Biotin)
 Tim-4 (小鼠):Fc (人) (重组) (生物素标记)		 10 µg – –
AG-40B-0180B-3010 Tim-4 (mouse):Fc (human) (rec.) (Biotin)
 Tim-4 (小鼠):Fc (人) (重组) (生物素标记)		 3 x 10 µg – –

GIP, human TFA(Synonyms: 抑胃肽三氟乙酸盐 (人) Gastric Inhibitory Peptide (GIP), human TFA)

发表于由

GIP, human TFA (Synonyms: 抑胃肽三氟乙酸盐 (人); Gastric Inhibitory Peptide (GIP), human TFA) 纯度: 96.24%

GIP, human TFA 是一种由 42 个氨基酸组成的肽类激素，是葡萄糖依赖性胰岛素分泌的促进剂和胃酸分泌的弱抑制剂。GIP, human TFA 作为肠 K 细胞释放的肠促胰岛素激素，对营养摄入作出反应。

GIP, human TFA&nbsp;(Synonyms: 抑胃肽三氟乙酸盐 (人); Gastric Inhibitory Peptide (GIP), human TFA)

GIP, human TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ￥5000 In-stock
5 mg   询价  
10 mg   询价  

* Please select Quantity before adding items.

GIP, human TFA 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

GIP, human TFA, a peptide hormone consisting of 42 amino acids, is a stimulator of glucose-dependent insulin secretion and a weak inhibitor of gastric acid secretion. GIP, human TFA acts as an incretin hormone released from intestinal K cells in response to nutrient ingestion[1][2][3].

体外研究
(In Vitro)

Gastric Inhibitory Polypeptide (GIP) exerts various peripheral effects on adipose tissue and lipid metabolism, thereby leading to increased lipid deposition in the postprandial state[1]. GIP, human plays a vital role in lipid metabolism and the development of obesity.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

5097.62

Formula

C228H339F3N60O68S
Sequence

Tyr-Ala-Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-His-Gln-Gln-Asp-Phe-Val-Asn-Trp-Leu-Leu-Ala-Gln-Lys-Gly-Lys-Lys-Asn-Asp-Trp-Lys-His-Asn-Ile-Thr-Gln
Sequence Shortening

YAEGTFISDYSIAMDKIHQQDFVNWLLAQKGKKNDWKHNITQ
中文名称

抑胃肽三氟乙酸盐 (人)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light, stored under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Meier JJ, et al. Gastric inhibitory polypeptide: the neglected incretin revisited. Regul Pept. 2002 Jul 15;107(1-3):1-13.

    [2]. Miyachi A, et al. Quantitative analytical method for determining the levels of gastric inhibitory polypeptides GIP1-42 and GIP3-42 in human plasma using LC-MS/MS/MS. J Proteome Res. 2013;12(6):2690-2699.

    [3]. Gabe MBN, et al. Molecular interactions of full-length and truncated GIP peptides with the GIP receptor – A comprehensive review. Peptides. 2020;125:170224.

  • [1]. Meier JJ, et al. Gastric inhibitory polypeptide: the neglected incretin revisited. Regul Pept. 2002 Jul 15;107(1-3):1-13.

    [2]. Miyachi A, et al. Quantitative analytical method for determining the levels of gastric inhibitory polypeptides GIP1-42 and GIP3-42 in human plasma using LC-MS/MS/MS. J Proteome Res. 2013;12(6):2690-2699.

    [3]. Gabe MBN, et al. Molecular interactions of full-length and truncated GIP peptides with the GIP receptor – A comprehensive review. Peptides. 2020;125:170224.

PAMP-12(human, porcine)

发表于由

PAMP-12(human, porcine)  纯度: 99.03%

PAMP-12(human, porcine) 是免疫反应性 (ir)-PAMP 的一种主要成分，由肾上腺髓质素前体加工而成，是一种有效的降压肽，可参与心血管方面的调控。

PAMP-12(human, porcine)&nbsp;

PAMP-12(human, porcine) Chemical Structure

CAS No. : 196305-05-2

规格 价格 是否有货 数量
1 mg ￥1200 In-stock
5 mg ￥3600 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

PAMP-12(human, porcine) 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

PAMP-12(human, porcine) is a major component of immunoreactive (ir)-PAMP, is processed from the adrenomedullin precursor, is a potent hypotensive peptide and participates in cardiovascular control[1].

体外研究
(In Vitro)

PAMP-12 (human, porcine) is the PAMP[9-20] with a C-terminal amide structure. Intravenous injections of PAMP-12 in anesthetized rats show a significant hypotensive effect in a dose-dependent fashion[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1618.93

Formula

C77H119N25O14
CAS 号

196305-05-2
Sequence Shortening

FRKKWNKWALSR-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80°C 2 years
-20°C 1 year
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. K Kuwasako, et al. Purification and Characterization of PAMP-12 (PAMP[9-20]) in Porcine Adrenal Medulla as a Major Endogenous Biologically Active Peptide. FEBS Lett. 1997 Sep 1;414(1):105-10.

  • [1]. K Kuwasako, et al. Purification and Characterization of PAMP-12 (PAMP[9-20]) in Porcine Adrenal Medulla as a Major Endogenous Biologically Active Peptide. FEBS Lett. 1997 Sep 1;414(1):105-10.