Phenol Red sodium salt(Synonyms: Phenolsulfonephthalein sodium salt)

发表于2024年4月11日由Whatman官网

Phenol Red sodium salt;(Synonyms: Phenolsulfonephthalein sodium salt) 纯度: ge;97.0%

Phenol red (Phenolsulfonephthalein) sodium salt 是一种 pH 指示染料，在出现阳性反应时，依赖于从粉红色到黄色的明显颜色变化。

Phenol Red sodium saltamp;;(Synonyms: Phenolsulfonephthalein sodium salt)

Phenol Red sodium salt Chemical Structure

CAS No. : 34487-61-1

规格	价格	是否有货	数量
10;mM;*;1 mL in DMSO	￥550	In-stock
500 mg	￥400	In-stock
5 g	￥550	In-stock
25 g	￥1100	In-stock
50 g	;	询价	;

* Please select Quantity before adding items.

Phenol Red sodium salt 相关产品

bull;相关化合物库:

Covalent Screening Library Plus
Bioactive Compound Library Plus
Covalent Screening Library
FDA Approved amp; Pharmacopeial Drug Library

生物活性

Phenol red (Phenolsulfonephthalein) sodium salt is a pH indicator dye, relying on a distinct color change from pink to yellow in case of a positive reaction^[1] ^[2].

分子量

376.36

Formula

C₁₉H₁₃NaO₅S

CAS 号

34487-61-1

中文名称

苯酚红钠盐；酚红钠盐；酚红钠

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:;

DMSO : 5 mg/mL (13.29 mM; Need ultrasonic)

H₂O : < 0.1 mg/mL (insoluble)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.6570 mL	13.2852 mL	26.5703 mL
5 mM	0.5314 mL	2.6570 mL	5.3141 mL
10 mM	0.2657 mL	1.3285 mL	2.6570 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

参考文献

[1]. Morgan A, et al. Caution for the routine use of phenol red – It is more than just a pH indicator. Chem Biol Interact. 2019;310:108739.

[2]. Peltzer D, et al. Rapid and simple colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Bovine alphaherpesvirus 1. J Virol Methods. 2021;289:114041.

Cell Assay ^[1]^[2]	Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 10⁵ cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time^[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Morgan A, et al. Caution for the routine use of phenol red – It is more than just a pH indicator. Chem Biol Interact. 2019;310:108739. [2]. Peltzer D, et al. Rapid and simple colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Bovine alphaherpesvirus 1. J Virol Methods. 2021;289:114041.

Cell Assay
^[1]^[2]

Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 10⁵ cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time^[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Morgan A, et al. Caution for the routine use of phenol red – It is more than just a pH indicator. Chem Biol Interact. 2019;310:108739.

[2]. Peltzer D, et al. Rapid and simple colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Bovine alphaherpesvirus 1. J Virol Methods. 2021;289:114041.