Parasin I TFA

发表于2024年1月22日由Whatman官网

Parasin I TFA; 纯度: 98.27%

Parasin I (TFA) 是一种由 19 个氨基酸残基组成的多肽，源于从鲶鱼皮肤分离到的组蛋白 H2A，具有抗菌的作用。

Parasin I TFA Chemical Structure

规格	价格	是否有货	数量
500 μg	￥700	In-stock
1 mg	￥1200	In-stock
5 mg	￥4800	In-stock
10 mg	;	询价	;
50 mg	;	询价	;

* Please select Quantity before adding items.

Parasin I TFA 相关产品

bull;相关化合物库:

Natural Product Library Plus
Bioactive Compound Library Plus
Anti-Infection Compound Library
Natural Product Library
Antibacterial Compound Library
Peptide Library

生物活性

Parasin I (TFA) is a 19-amino acid histone H2A-derived peptide isolated from the skin of the catfish, and shows antimicrobial activity^[1].

IC₅₀ Target

Bacterial^[1]

分子量

2114.33

Formula

C₈₂H₁₅₄N₃₄O₂₄.C₂HF₃O₂

Sequence

Lys-Gly-Arg-Gly-Lys-Gln-Gly-Gly-Lys-Val-Arg-Ala-Lys-Ala-Lys-Thr-Arg-Ser-Ser

Sequence Shortening

KGRGKQGGKVRAKAKTRSS

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder	-80deg;C	2 years
	-20deg;C	1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

Solvent Solubility

In Vitro:;

H₂O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

;	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3.;;Recommended solution:

Overall charge of peptide	Details
Negative (lt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH₄OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

参考文献

[1]. Koo YS, et al. Structure-activity relations of parasin I, a histone H2A-derived antimicrobial peptide. Peptides. 2008 Jul;29(7):1102-8.

Parasin I(Synonyms: 鲶鱼抗菌肽 I)

发表于2024年1月12日由Whatman官网

Parasin I;(Synonyms: 鲶鱼抗菌肽 I)

Parasin I 是一种由 19 个氨基酸残基组成的多肽，源于从鲶鱼皮肤分离到的组蛋白 H2A，具有抗菌的作用。

Parasin I Chemical Structure

CAS No. : 219552-69-9

规格		是否有货
5 mg		询价
10 mg		询价
25 mg		询价

* Please select Quantity before adding items.

生物活性	Parasin I is a 19-amino acid histone H2A-derived peptide isolated from the skin of the catfish, and shows antimicrobial activity.
体外研究 (In Vitro)	Parasin I with comparable antimicrobial activities localized to the cell membrane and subsequently permeabilized the outer and cytoplasmic membranes. Parasin I and its active analogs show strong cytoplasmic membrane permeabilizing activity^[1]. Codon optimized parasin I fused with human lysozyme is expressed in Pichia pastoris, and has potent antibiotic activity^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	2000.31
Formula	C₈₂H₁₅₄N₃₄O₂₄
CAS 号	219552-69-9
Sequence	Lys-Gly-Arg-Gly-Lys-Gln-Gly-Gly-Lys-Val-Arg-Ala-Lys-Ala-Lys-Thr-Arg-Ser-Ser
Sequence Shortening	KGRGKQGGKVRAKAKTRSS
中文名称	鲶鱼抗菌肽 I
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Koo YS, et al. Structure-activity relations of parasin I, a histone H2A-derived antimicrobial peptide. Peptides. 2008 Jul;29(7):1102-8. [2]. Zhao H, et al. Characterization of bioactive recombinant antimicrobial peptide parasin I fused with human lysozyme expressed in the yeast Pichia pastoris system. Enzyme Microb Technol. 2015 Sep;77:61-7.

Cell Assay ^[1]	The antimicrobial activity of each peptide is determined using the broth microdilution assay. Briefly, single colonies of bacteria and fungi are inoculated into 3% trypticase soy broth (TSB) and Saboraud’s medium, respectively, and cultured overnight at 37 and 30°C, respectively. Aliquots of each culture are transferred to 50 mL of fresh medium and incubated for an additional 3-6 h to obtain midlogarithmic phase cells. The cells are then washed and resuspended in 10 mM sodium phosphate buffer (NAPB), pH 7.4. The relationship between absorbance at 620 nm and colony-forming units (cfus) is determined for each microorganism by spreading serial dilutions of the cell suspension onto TSB or Saboraud agar plates. The cell suspension is diluted to 5×10⁵ cfu/mL with 10 mM NAPB. Each well of 96- well propylene microtiter plates is filled with 90 mL of the diluted suspension and 10 mL of serially diluted peptide samples. After incubation for 3 h, fresh medium is added to the mixture and incubated at 37°C (bacteria) or 30°C (fungi) for an additional 16 h. The inhibition of growth is determined by measuring absorbance at 620 nm with a Model 550 Microplate Reader. The lowest concentration of peptide that completely inhibits growth is defined as the ‘minimal inhibitory concentration’ (MIC). The MICs are the average values obtained in triplicates in three independent experiments. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Koo YS, et al. Structure-activity relations of parasin I, a histone H2A-derived antimicrobial peptide. Peptides. 2008 Jul;29(7):1102-8. [2]. Zhao H, et al. Characterization of bioactive recombinant antimicrobial peptide parasin I fused with human lysozyme expressed in the yeast Pichia pastoris system. Enzyme Microb Technol. 2015 Sep;77:61-7.

Cell Assay
^[1]

The antimicrobial activity of each peptide is determined using the broth microdilution assay. Briefly, single colonies of bacteria and fungi are inoculated into 3% trypticase soy broth (TSB) and Saboraud’s medium, respectively, and cultured overnight at 37 and 30°C, respectively. Aliquots of each culture are transferred to 50 mL of fresh medium and incubated for an additional 3-6 h to obtain midlogarithmic phase cells. The cells are then washed and resuspended in 10 mM sodium phosphate buffer (NAPB), pH 7.4. The relationship between absorbance at 620 nm and colony-forming units (cfus) is determined for each microorganism by spreading serial dilutions of the cell suspension onto TSB or Saboraud agar plates. The cell suspension is diluted to 5×10⁵ cfu/mL with 10 mM NAPB. Each well of 96- well propylene microtiter plates is filled with 90 mL of the diluted suspension and 10 mL of serially diluted peptide samples. After incubation for 3 h, fresh medium is added to the mixture and incubated at 37°C (bacteria) or 30°C (fungi) for an additional 16 h. The inhibition of growth is determined by measuring absorbance at 620 nm with a Model 550 Microplate Reader. The lowest concentration of peptide that completely inhibits growth is defined as the ‘minimal inhibitory concentration’ (MIC). The MICs are the average values obtained in triplicates in three independent experiments.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Koo YS, et al. Structure-activity relations of parasin I, a histone H2A-derived antimicrobial peptide. Peptides. 2008 Jul;29(7):1102-8.

[2]. Zhao H, et al. Characterization of bioactive recombinant antimicrobial peptide parasin I fused with human lysozyme expressed in the yeast Pichia pastoris system. Enzyme Microb Technol. 2015 Sep;77:61-7.

Parasin I 品牌：Anygen

发表于2023年12月6日由Whatman官网

Parasin I

品牌：Anygen
CAS No.：
储存条件：-20℃
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
307-92271	–	0.5 mg	–	咨询	–	–

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