Genipin 1-β-D-gentiobioside (Genipin 1-gentiobioside) is one of the most abundant and bioactive iridoid glycosides in Gardenia jasminoides Ellis, which possesses hepatoprotective, anti-inflammatory, antioxidant, and antithrombotic activities.

Genipin 1-β-D-gentiobioside (Genipin gentiobioside) has moderate terminal elimination half-life (t_1/2=1.65±0.87 h and 2.43±2.30 h for nomal rats (11.4 mg/kg) and cholestatic liver injury (CLI) rats (11.4 mg/kg), respectively)^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

550.51

C₂₃H₃₄O₁₅

29307-60-6

京尼平-1-β-D-龙胆双糖苷；京尼平龙胆双糖苷

Room temperature in continental US; may vary elsewhere.

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

Ethanol : 125 mg/mL (227.06 mM; Need ultrasonic)

DMSO : 100 mg/mL (181.65 mM; Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

  Solubility: ≥ 2.5 mg/mL (4.54 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (4.54 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2.5 mg/mL (4.54 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (4.54 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

  Solubility: ≥ 2.5 mg/mL (4.54 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (4.54 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Zhu H, et al. Simultaneous determination of two iridoid glycosides, two anthraquinones and four flavonoid glycosides of Zhi-Zi-Da-Huang decoction in rat plasma by UFLC-MS/MS: application to a comparative pharmacokinetic study in normal and cholestatic liver injury rats. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jun 1;960:116-25.

Rats^[1]
Twelve male rats are divided randomly into normal and acute cholestatic liver injury (CLI) group, with 6 rats in each. The rats are kept in an air-conditioned environment at a temperature of 22-24°C and a relative humidity of 50±10%, with access to the standard laboratory food and water. After fasted for 12 h, the rats are given Zhi-Zi-Da-Huang decoction (ZZDHD) at a dose of 8 g/kg (crude drug/body weight, equivalent to 35.9 mg/kg geniposide, 11.4 mg/kg Genipin 1-β-D-gentiobioside, 2.50 mg/kg Rhein, 0.23 mg/kg Emodin, 11.2 mg/kg Isonaringin, 83.5 mg/kg Naringin, 12.4 mg/kg Hesperidin and 79.6 mg/kg Neohesperidin) by oral administration. Blood samples (approximately 0.3 mL) are collected from the fosse orbital vein using heparinized 1.5 mL polythene tubes before the dose and at 0.08, 0.17, 0.33, 0.67, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36 and 48 h after dosing, and then centrifuged immediately at 13,000 rpm for 5 min. Plasma is transferred into clean tubes and stored at -80°C until analysis^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Zhu H, et al. Simultaneous determination of two iridoid glycosides, two anthraquinones and four flavonoid glycosides of Zhi-Zi-Da-Huang decoction in rat plasma by UFLC-MS/MS: application to a comparative pharmacokinetic study in normal and cholestatic liver injury rats. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jun 1;960:116-25.