Ruthenium red (Ammoniated ruthenium oxychloride) is a polycationic dye widely used for electron microscopy (EM) of cells, tissues and vegetative bacteria. Ruthenium red strongly reacts with phospholipids and fatty acids and binds to acidic mucopolysaccharides. Ruthenium red is a L-type calcium current (I_Ca) blocker^[1][2].

I_Ca^[2]

Ruthenium red staining of intracellular organelles and structures seemed to depend on the time of exposure to this agent, as well on the concentration used^[2].
Ruthenium red efficiently blocks the L-type calcium current in a dose-dependent manner in in guinea-pig isolated ventricular heart cells, with EC₅₀ of 0.8 μM^[2].
Ruthenium red (10 μM) blocks the sarcoplasmic Ca release channels or the mitochondrial Ca uptake, and blocks 26.7 % of the sodium current, and slows its inactivation time-course in guinea-pig isolated ventricular heart cells^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

11103-72-3

Room temperature in continental US; may vary elsewhere.

H₂O : 50 mg/mL (Need ultrasonic)

DMSO : 10 mg/mL (Need ultrasonic)

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

  Solubility: 1 mg/mL (Infinity mM); Clear solution; Need ultrasonic

  此方案可获得 1 mg/mL (Infinity mM) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 10.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

• [1]. Lashanda N Waller, et al. Ruthenium red staining for ultrastructural visualization of a glycoprotein layer surrounding the spore of Bacillus anthracis and Bacillus subtilis. J Microbiol Methods. 2004 Jul;58(1):23-30.

  [2]. Claire O Malécot, et al. Ruthenium red as an effective blocker of calcium and sodium currents in guinea-pig isolated ventricular heart cells. Br J Pharmacol. 1998 Jun; 124(3): 465-472.