标签归档：nm

Pepstatin Ammonium(Synonyms: Pepstatin A Ammonium)

发表于由

Pepstatin Ammonium;(Synonyms: Pepstatin A Ammonium) 纯度: 99.66%

Pepstatin Ammonium 是由放线菌类产生的一种特异性的天冬氨酸蛋白酶 (aspartic proteases) 抑制剂，能够抑制 hemoglobin-pepsin，hemoglobin-proctase，casein-pepsin，casein-proctase，casein-acid protease 和 hemoglobin-acid protease 的活性，IC50 值分别为 4.5 nM，6.2 nM，150 nM，290 nM，520 nM 和 260 nM；同时可抑制 HIV protease 的活性。

Pepstatin Ammoniumamp;;(Synonyms: Pepstatin A Ammonium)

Pepstatin Ammonium Chemical Structure

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10 mg ￥550 In-stock
25 mg ￥1200 In-stock
50 mg ￥1950 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

Pepstatin Ammonium 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Pepstatin Ammonium is a specific aspartic protease inhibitor produced by actinomycetes, with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease, respectively. Pepstatin Ammonium also inhibits HIV protease.

IC50 Target

IC50: 4.5 nM (Hemoglobin-pepsin), 6.2 nM (Hemoglobin-proctase), 150 nM (Casein-pepsin), 260 nM (Hemoglobin-acid protease), 290 nM (Casein-proctase), 520 nM (Casein-acid protease)[1]
体外研究
(In Vitro)

Pepstatin Ammonium is a specific acid protease inhibitor produced by actinomycetes, with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM for hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease, respectively[1]. Pepstatin (Pepstatin A) inhibits the recombinant HIV protease with an IC50 of 250 μM. Pepstatin shows no effect on cellular protein synthesis and probably does not exert severe cell toxicity[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Pepstatin has a very low toxicity, with LD50s of 1090 mg/kg, 875 mg/kg, 820 mg/kg and 450 mg/kg for mice, rats, rabbits, and dogs by i.p. route, and >2000 mg/kg for all species by oral route. Pepstatin (0.5-50 mg/kg, p.o.) suppresses stomach ulceration of the pylorus in ligated Shay rats[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

702.92

Formula

C34H66N6O9
中文名称

抑肽素铵
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 41.67 mg/mL (59.28 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.4226 mL 7.1132 mL 14.2264 mL
5 mM 0.2845 mL 1.4226 mL 2.8453 mL
10 mM 0.1423 mL 0.7113 mL 1.4226 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (2.96 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.96 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (2.96 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (2.96 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (2.96 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.96 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Umezawa H, et al. Pepstatin, a new pepsin inhibitor produced by Actinomycetes. J Antibiot (Tokyo). 1970 May;23(5):259-62.

    [2]. Seelmeier S, et al. Human immunodeficiency virus has an aspartic-type protease that can be inhibited by pepstatin A. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6612-6.
Cell Assay
[2]

Pepstatin A is freshly dissolved in DMSO at 7 mM. It is very slowly diluted (1:100) into the medium of HIV-infected H9 suspension cultures so that no pepstatin A precipitated (final concentration, 70 μM pepstatin A and 1% DMSO), and the cultures are incubated without change of culture medium for 48 hr. As control, uninfected H9 cells are also incubated with pepstatin and in addition HIV infected and uninfected cells are incubated with 1% DMSO but without pepstatin[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Umezawa H, et al. Pepstatin, a new pepsin inhibitor produced by Actinomycetes. J Antibiot (Tokyo). 1970 May;23(5):259-62.

    [2]. Seelmeier S, et al. Human immunodeficiency virus has an aspartic-type protease that can be inhibited by pepstatin A. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6612-6.

荧光染料Indo-1 AM(Synonyms: Indo-1 Acetoxymethyl ester)

发表于由

荧光染料Dye Reagents Indo-1 AM;(Synonyms: Indo-1 Acetoxymethyl ester)

Indo-1 AM 是一种荧光 Ca2+ 指示剂 (λex=340 nm，λem=405/485 nm)。

Indo-1 AM(Synonyms: Indo-1 Acetoxymethyl ester)

Indo-1 AM Chemical Structure

CAS No. : 112926-02-0

规格 价格 是否有货
1 mg ￥6151 询问价格 货期

* Please select Quantity before adding items.

生物活性

Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).

体外研究
(In Vitro)

Indo-1 AM is a fluorescent Ca2+ indicator with λex=340 nm and λem=405/485 nm[1]. In rabbit hearts that are exposed to Indo-1 AM, 72% of the dye is in the cytosol, and the remainder is associated with the mitochondria, microsomes and the nucleus[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1009.91

Formula

C47H51N3O22
CAS 号

112926-02-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Andrienko T, et al. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning. PLoS One. 2016 Dec 1;11(12):e0167300.

    [2]. Blatter LA, et al. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys J. 1990 Dec;58(6):1491-9.
Cell Assay
[2]

The cells are loaded with indo-I and fura-2 by exposure to the membrane-permeant forms, Indo-1 AM (indo-l-acetoxymethyl ester) and fura-2-acetoxymethyl ester, respectively. A mixture of 10 μL of 1 mM Indo-1 AM or fura-2/AM in dimethylsulfoxide (DMSO), 2.5 μL of 25% wt/wt Pluronic F-127 in DMSO, and 75 μL fetal calf serum (FCS) or newborn calf serum (NCS) is added to 2 mL Tyrode solution containing the cells and 40 μL FCS or NCS. Loading is achieved by gently shaking the cells for 15 to 30 min[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Andrienko T, et al. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning. PLoS One. 2016 Dec 1;11(12):e0167300.

    [2]. Blatter LA, et al. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys J. 1990 Dec;58(6):1491-9.

荧光染料ADHP(Synonyms: 10-Acetyl-3,7-dihydroxyphenoxazine)

发表于由

荧光染料Dye Reagents ADHP;(Synonyms: 10-Acetyl-3,7-dihydroxyphenoxazine)

ADHP 是一种荧光过氧化物酶底物（λex=530 nm, λem=590 nm）。

ADHP(Synonyms: 10-Acetyl-3,7-dihydroxyphenoxazine)

ADHP Chemical Structure

CAS No. : 119171-73-2

规格 价格 是否有货
5 mg ￥2200 询问价格 货期

* Please select Quantity before adding items.

生物活性

ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

体外研究
(In Vitro)

To obtain the parameters Km and kcat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6 s−1.The kobs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

257.24

Formula

C14H11NO4
CAS 号

119171-73-2
中文名称

10-乙酰基-3,7-二羟基吩嗪
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Jiansheng Huang, et al. Ordered Cleavage of Myeloperoxidase Ester Bonds Releases Active site Heme Leading to Inactivation of Myeloperoxidase by Benzoic Acid Hydrazide Analogs. Arch Biochem Biophys. 2014 Apr 15; 548: 74–85.
Kinase Assay
[1]

ADHP, 4-ABAH, 2-ABAH, 4-BAH, 4-FBAH, 4-NBAH, 4-TFMBAH, 3-DMABAH, NaN3 and isoniazid are dissolved in DMSO and subsequently diluted into assay buffer. The final concentration of DMSO in the reaction is less than 0.5 % (v/v), which does not affect fluorescence of the oxidized ADHP product 7-hydroxyl-3H-phenoxazin-3-one (resorufin). Reactions of ADHP (20 μM) are incubated with MPO (2.8 nm) in assay buffer and initiated by the addition of 1/10th volume H2O2 from a serial dilution basin. To determine the effect that the simplest benzoic acid hydrazide inhibitor or its analog 4-TFMBAH has on the heme catalytic ability of MPO, MPO (1.2 μM) is incubated for 10 min with different concentrations of BAH inhibitor (0, 0.025, 0.25, 2.5, 12.5 and 25 mM) with ADHP (40 μM) and timing of the reaction is measured following addition of H2O2 (20 μM) ADHP. All reactions are measured in assay buffer at room temperature. Samples of 20 μL are added to non-reducing sample loading buffers, and then loaded without prior heating and resolved by 4-15% gradient SDS-polyacrylamide gel electrophoresis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Jiansheng Huang, et al. Ordered Cleavage of Myeloperoxidase Ester Bonds Releases Active site Heme Leading to Inactivation of Myeloperoxidase by Benzoic Acid Hydrazide Analogs. Arch Biochem Biophys. 2014 Apr 15; 548: 74–85.

Cy7.5

发表于由

Cy7.5;

Cy7.5 是一种荧光标记试剂 (Ex=700-770 nm，Em=790 nm)。Cyanine 染料可用于标记蛋白质，抗体，肽，寡聚核苷酸等。

Cy7.5amp;;

Cy7.5 Chemical Structure

CAS No. : 847180-48-7

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Cy7.5 is a fluorescence imaging (FI) agent (Ex=700-770 nm，Em=790 nm) as well as a magnetic resonance imaging (MRI) imaging agent. Cyanine dyes are used to label proteins, antibodies, peptides, and oligonucleotide[1].

分子量

943.09

Formula

C43H46N2O14S4
CAS 号

847180-48-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
溶解性数据
In Vitro:;

DMSO : 62.5 mg/mL (66.27 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0603 mL 5.3017 mL 10.6034 mL
5 mM 0.2121 mL 1.0603 mL 2.1207 mL
10 mM 0.1060 mL 0.5302 mL 1.0603 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (2.21 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.21 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (2.21 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (2.21 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Zhan Y, et al. In Vivo Dual-Modality Fluorescence and Magnetic Resonance Imaging-Guided Lymph Node Mapping with Good Biocompatibility Manganese Oxide Nanoparticles. Molecules. 2017 Dec 12;22(12). pii: E2208.

Research Diets动物饲料98121701NM Formula 模型饲料配方表

发表于由

上海金畔生物科技有限公司代理Research Diets品牌动物饲料全线产品，欢迎访问官网了解更多产品信息。
Research Diets动物饲料98121701NM Formula 模型饲料配方表

98121701NM

RD Low Fat Diet Without Cholesterol

适用实验动物模型: 啮齿类 (大鼠/小鼠)
饲料颜色: 未添加染色剂
是否辐照处理/辐照产品号码 :

Research Diets生产饲料大部分都可以送伽马辐照。我们会在这类的产品号结尾标注 “i” 字母以说明其为通过辐照处理的饲料，而饲料经过多轮辐照处理则会标示双字母 “ii” 以区别单轮辐照 “i”。如果您有任何关于辐照的问题，请联系我们的科研技术支持。

无

纯化饲料配方表

营养成分类别 成分 公克
蛋白质 酪蛋白（乳酸; 30筛孔） 195.00 克
蛋白质 Methionine, DL 3.00 克
碳水化合物 玉米淀粉 404.40 克
碳水化合物 蔗糖(微粒) 341.00 克
碳水化合物 麦芽糊精 100.00 克
纤维素 纤维素(Solka Floc/FCC200) 50.00 克
脂肪 玉米油 52.50 克
矿物质 S10001 35.00 克
矿物质 碳酸钙, light(USP) 4.00 克
维他命 V10001 10.00 克
维他命 酒石酸胆碱 2.00 克
抗氧化剂 Ethoxyquin 0.04 克
饲料配方总重（公克）: 1196.94 克