Colivelin;
Colivelin 是一种具有大脑通透性的神经保护肽 (neuroprotective peptide)，是 STAT3 的有效激活剂。Coliveli 通过激活 STAT3 在体外抑制神经元死亡。Colivelin 对神经毒性、Aβ 沉积,神经元细胞凋亡,在神经退行性疾病显示出长期有利的影响。Colivelin 具有潜力用于阿尔茨海默病和缺血性脑损伤的相关研究。
Colivelin Chemical Structure
CAS No. : 867021-83-8
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| 100 mg |
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| 250 mg |
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| 500 mg |
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* Please select Quantity before adding items.
Colivelin 的其他形式现货产品:
Colivelin TFA
| 生物活性 |
Colivelin is a brain penetrant neuroprotective peptide and a potent activator of STAT3, suppresses neuronal death by activating STAT3 in vitro[1]. Colivelin exhibits long-term beneficial effects against neurotoxicity, Aβ deposition, neuronal apoptosis, and synaptic plasticity deficits in neurodegenerative disease[2]. Colivelin has the potential for the treatment of alzheimer’s disease and ischemic brain injury[1]
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| IC50 Target |
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体外研究
(In Vitro) |
Colivelin completely suppresses death induced by overexpressed FAD-causative genes and Aβ1-43 at a concentration of 100 fm, and keep its neuroprotective action at or above the levels of 1 nm[1].Colivelin-induced neuroprotection occurs via two neuroprotective pathways: one mediated by Ca2+/calmodulin-dependent protein kinase IV, triggered by ADNF, and one mediated by signal transducer and activator of transcription 3, triggered by HN[1]. Colivelin reverses caspase3, Bax and Bcl-2 expressions in HT22 cells medaited by rmMFG-E8 in the co-cultured cells under OGD condition[4].
Colivelin (50 µg/mL, 4 hours) significantly increases the p-STAT3 protein levels in BV-2 cells[4].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis[4]
| Cell Line: |
BV-2 cells. |
| Concentration: |
50 µg/mL. |
| Incubation Time: |
4 hours. |
| Result: |
Increased p-STAT3 levels. |
Cell Viability Assay[5]
| Cell Line: |
KYSE70 and TE8 cells. |
| Concentration: |
0.5 μM. |
| Incubation Time: |
1 hour (followed by CYT-Rx20 treatment) |
| Result: |
Sgnificantly suppressed the viability in KYSE70 and TE8 cells. |
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体内研究
(In Vivo) |
Colivelin (intracerebroventricular administration; 10 pmol/3 μl; 3 weeks) suppresses impairment in spatial working memory induced by repetitive intracerebroventricular injection of Aβ25-35 or Aβ1-42, in addition, it antagonizes neuronal loss in the CA1 region of hippocampus induced by hippocampal injection of Aβ1-42[1]. Colivelin (intraperitoneal administration; 1.4, 7, or 35 nM/0.21 mL; on the Y-maze testday) suppresses memory impairment caused by 3-quinuclidinyl benzilateand restricts functional memory deficit[1]. Colivelin (intraperitoneal injection; 1 mg/kg; 14 days) results in improved motor and cognitive function with time by performance of mNSS, rotarod, and corner turning test.It also reduces lesion volume and improves neurological deficits after MCAO[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: |
CD-1 mice[1] |
| Dosage: |
10 pmol/3 μl |
| Administration: |
Intracerebroventricular administration |
| Result: |
Completely suppressed Aβ 25-35-mediated impairment in spatial working memory and increased the number of immunoreactive neurons. |
| Animal Model: |
C57 mice[1] |
| Dosage: |
1.4, 7, or 35 nM/0.21mL |
| Administration: |
Intraperitoneal administration |
| Result: |
Protected against cholinotoxin-induced amnesia in mice. |
| Animal Model: |
Male C57BL/6 mice[3] |
| Dosage: |
1 mg/kg |
| Administration: |
Intraperitoneal administration |
| Result: |
Protected against ischemic brain injury, and improves neurological outcomes |
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| 分子量 |
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| Formula |
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| CAS 号 |
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| Sequence Shortening |
SALLRSIPAPAGASRLLLLTGEIDLP
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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| 参考文献 |
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[1]. Chiba T, et al. Development of a femtomolar-acting humanin derivative named colivelin by attaching activity-dependent neurotrophic factor to its N terminus: characterization of colivelin-mediated neuroprotection against Alzheimer’s disease-relevant insults in vitro and in vivo. J Neurosci. 2005 Nov 2;25(44):10252-61.
[2]. Pan Z, et al. Upregulation of HSP72 attenuates tendon adhesion by regulating fibroblast proliferation and collagen production via blockade of the STAT3 signaling pathway.Cell Signal. 2020 Mar 18:109606.
[3]. Zhao H, et al. Colivelin Rescues Ischemic Neuron and Axons Involving JAK/STAT3 Signaling Pathway.Neuroscience. 2019 Sep 15;416:198-206.
[4]. Fang YY, et al. MFG-E8 alleviates oxygen-glucose deprivation-induced neuronal cell apoptosis by STAT3 regulating the selective polarization of microglia. Int J Neurosci. 2020 Mar 12:1-10.
[5]. Chiu WC, et al. The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways. PLoS One. 2016 Nov 22;11(11):e0166453.
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