荧光染料EDANS(Synonyms: 1,5-EDANS)

荧光染料Dye Reagents EDANS;(Synonyms: 1,5-EDANS) 纯度: 99.95%

EDANS (1,5-EDANS) 是一种新型的淬灭荧光底物 (fluorogenic substrate),用于通过共振能量转移分析逆转录病毒蛋白酶。

EDANSamp;;(Synonyms: 1,5-EDANS)

EDANS Chemical Structure

CAS No. : 50402-56-7

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥550 In-stock
100 mg ¥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

EDANS 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET)[1].

体外研究
(In Vitro)

The peptide sequence of EDANS is derived from a natural processing site for HIV-1 PR[1].
Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that was linearly related to the extent of substrate hydrolysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

266.32

Formula

C12H14N2O3S

CAS 号

50402-56-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

DMSO : 13.89 mg/mL (52.16 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.7549 mL 18.7744 mL 37.5488 mL
5 mM 0.7510 mL 3.7549 mL 7.5098 mL
10 mM 0.3755 mL 1.8774 mL 3.7549 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 1.39 mg/mL (5.22 mM); Clear solution

    此方案可获得 ≥ 1.39 mg/mL (5.22 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 13.9 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.39 mg/mL (5.22 mM); Clear solution

    此方案可获得 ≥ 1.39 mg/mL (5.22 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 13.9 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 1.39 mg/mL (5.22 mM); Clear solution

    此方案可获得 ≥ 1.39 mg/mL (5.22 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 13.9 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. E D Matayoshi, et al. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. Science. 1990 Feb 23;247(4945):954-8.

DiI(Synonyms: DiIC18(3))

DiI;(Synonyms: DiIC18(3)) 纯度: 99.92%

DiI (DiIC18(3)) 是一种亲脂性膜染料。DiI 在进入细胞膜之前荧光非常弱,仅当进入到细胞膜后才可以被激发出很强的荧光。DiI 被激发后可以发出橙红色的荧光,最大激发波长为 549 nm,最大发射波长为 565 nm。通常用作神经元和其它细胞的长期示踪剂。

DiIamp;;(Synonyms: DiIC18(3))

DiI Chemical Structure

CAS No. : 41085-99-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥605 In-stock
10 mg ¥550 In-stock
25 mg ¥1000 In-stock
50 mg ¥1500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

DiI 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

DiI (DiIC18(3)) is a lipophilic carbocyanine fluorescent dye for membrane labeling. DiI has an absorption maximum at 549 nm and an emission maximum 565 nm. DiI is mildly fluorescent in aqueous suspension, but becomes bright when bound to cell membrane.

体外研究
(In Vitro)

Once bound to a membrane it diffuses laterally in 2 dimensions; if there is no diffusion barrier, DiI generally stains the whole leaflet (one surface) of a biological membrane rapidly, but does not readily flip across to the other leaflet.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

DiI-labeled motoneurons have remained viable for up to 4 weeks in culture and up to one year in vivo[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

933.87

Formula

C59H97ClN2O4

CAS 号

41085-99-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 12.5 mg/mL (13.39 mM; ultrasonic and warming and heat to 80°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0708 mL 5.3541 mL 10.7081 mL
5 mM 0.2142 mL 1.0708 mL 2.1416 mL
10 mM 0.1071 mL 0.5354 mL 1.0708 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 1.25 mg/mL (1.34 mM); Suspended solution; Need ultrasonic

    此方案可获得 1.25 mg/mL (1.34 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 1.25 mg/mL (1.34 mM); Suspended solution; Need ultrasonic

    此方案可获得 1.25 mg/mL (1.34 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. D P Kuffler, et al. Long-term survival and sprouting in culture by motoneurons isolated from the spinal cord of adult frogs. J Comp Neurol. 1990 Dec 22;302(4):729-38.

荧光染料EDANS(Synonyms: 1,5-EDANS)

荧光染料Dye Reagents EDANS;(Synonyms: 1,5-EDANS) 纯度: 99.95%

EDANS (1,5-EDANS) 是一种新型的淬灭荧光底物 (fluorogenic substrate),用于通过共振能量转移分析逆转录病毒蛋白酶。

EDANS(Synonyms: 1,5-EDANS)

EDANS Chemical Structure

CAS No. : 50402-56-7

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥550 In-stock
100 mg ¥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

EDANS 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET)[1].

体外研究
(In Vitro)

The peptide sequence of EDANS is derived from a natural processing site for HIV-1 PR[1].
Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that was linearly related to the extent of substrate hydrolysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

266.32

Formula

C12H14N2O3S

CAS 号

50402-56-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

DMSO : 13.89 mg/mL (52.16 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.7549 mL 18.7744 mL 37.5488 mL
5 mM 0.7510 mL 3.7549 mL 7.5098 mL
10 mM 0.3755 mL 1.8774 mL 3.7549 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 1.39 mg/mL (5.22 mM); Clear solution

    此方案可获得 ≥ 1.39 mg/mL (5.22 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 13.9 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.39 mg/mL (5.22 mM); Clear solution

    此方案可获得 ≥ 1.39 mg/mL (5.22 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 13.9 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 1.39 mg/mL (5.22 mM); Clear solution

    此方案可获得 ≥ 1.39 mg/mL (5.22 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 13.9 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. E D Matayoshi, et al. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. Science. 1990 Feb 23;247(4945):954-8.

荧光染料HBC525

荧光染料Dye Reagents HBC525;

HBC525 是一种类 HBC 荧光团和荧光 RNA 适体 (Kd=3.8 nM)。HBC525 可以直接作为融合标签用于感兴趣的细胞 RNA 的成像和跟踪。荧光 RNA 适体也被用于构建各种有趣的用于细胞靶点检测和成像的动态 RNA 纳米工具。

HBC525

HBC525 Chemical Structure

CAS No. : 2174014-64-1

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

HBC525 is a HBC-like fluorophore and a fluorogenic RNA aptamer (Kd=3.8 nM). HBC525 can be directly used as fusion tags for the imaging and tracking of cellular RNAs of interest. Fluorogenic RNA aptamers have also been used to construct various interesting dynamic RNA nanodevices for cellular target detection and imaging[1][2].

IC50 Target

HBC525: 491/525 nm (Ex/Em)[2]

体内研究
(In Vivo)

荧光RNA适体

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

319.36

Formula

C19H17N3O2

CAS 号

2174014-64-1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Chen X, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293.

    [2]. Yu Q, et al. Genetically encoded RNA nanodevices for cellular imaging and regulation. Nanoscale. 2021;13(17):7988-8003.

Fluorescein Diacetate(Synonyms: 荧光素二乙酸酯; 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein)

生化分析试剂 Biochemical Assay Reagents
Fluorescein Diacetate;(Synonyms: 荧光素二乙酸酯; 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein) 纯度: 99.91%

Fluorescein diacetate 是一种细胞渗透性的酯酶底物。Fluorescein diacetate 可作为人谷胱甘肽硫转移酶 Pi (hGSTP1-1) 的荧光底物。

Fluorescein Diacetate(Synonyms: 荧光素二乙酸酯; 3,6-Diacetoxyfluoran;  Di-O-acetylfluorescein)

Fluorescein Diacetate Chemical Structure

CAS No. : 596-09-8

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ¥500 In-stock
500 mg ¥400 In-stock
5 g ¥500 In-stock
10 g ; 询价 ;
50 g ; 询价 ;

* Please select Quantity before adding items.

Fluorescein Diacetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.

体外研究
(In Vitro)

Fluorescein diacetate (FDA) is an acetylated derivative of the green fluorescent dye fluorescein[1]. Fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human Pi-class glutathione S-transferase (hGSTP1) selectively among various cytosolic GSTs. Fluorescence activation of Fluorescein diacetate susceptible to GST inhibitors is observed in MCF7 cells exogenously overexpressing hGSTP1, but not in cells overexpressing hGSTA1 or hGSTM1. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. To investigate whether the fluorescence activation is due to hGSTP1 activity, Fluorescein diacetate is incubated with recombinant hGSTP1-1 and GSH in vitro. Remarkable fluorescence activation is observed in the presence of both hGSTP1-1 and GSH, whereas only slight activation is observed in the absence of either of them or when the enzyme is heat inactivated. This suggests that the fluorescence activation of Fluorescein diacetate depends on hGSTP1-1 activity. From the linear relationship between the rate of increase in fluorescence and the hGSTP1-1 concentration, the specific activity of the enzyme for 1 μM Fluorescein diacetate is determined to be 79±15 nmol/min/mg protein. Fluorescein diacetate is applicable as a fluorogenic substrate for evaluating inhibitors of GSTP1-1 in vitro. For Fluorescein diacetate as a substrate, both Ethacrynic acid (EA) and NBDHEX suppress the hGSTP1-1-dependent fluorescent increase in a concentration-dependent manner, with IC50s of 3.3±0.5 μM and 0.61±0.04 μM, respectively[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

416.38

Formula

C24H16O7

CAS 号

596-09-8

中文名称

二乙酸荧光素;荧光素二乙酸盐;荧光素二乙酸酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 26.67 mg/mL (64.05 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.4017 mL 12.0083 mL 24.0165 mL
5 mM 0.4803 mL 2.4017 mL 4.8033 mL
10 mM 0.2402 mL 1.2008 mL 2.4017 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.00 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.00 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84.

    [2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.

Cell Assay
[2]

MCF7 cells (2-3×105) are seeded in a 35-mm glass bottom dish before the experiment. Prior to imaging, cells are washed with 1 mL PBS, then incubated in 1 mL Hanks’ Balanced Salt solution (HBSS(+) without phenol red) containing 1 μM Fluorescein diacetate (0.1% DMSO as a cosolvent) for 5 min at 37°C. Cells are washed twice with 1 mL PBS and 1 mL HBSS is added before imaging. Fluorescence images of fluorescein and DsRed-Express2 were acquired in the FITC channel (excitation at 473 nm) and the DsRed channel (excitation 559 nm). The 16-bit images obtained are analysed[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84.

    [2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.

荧光染料6-Aminofluorescein(Synonyms: 6-氨基荧光素 6-AF)

荧光染料Dye Reagents 6-Aminofluorescein;(Synonyms: 6-氨基荧光素; 6-AF) 纯度: 98.63%

6-Aminofluorescein (6-AF) 是一种新型荧光标记物。

6-Aminofluoresceinamp;;(Synonyms: 6-氨基荧光素; 6-AF)

6-Aminofluorescein Chemical Structure

CAS No. : 51649-83-3

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥770 In-stock
100 mg ¥700 In-stock
500 mg ¥1600 In-stock
1 g ¥2600 In-stock
5 g ; 询价 ;
10 g ; 询价 ;

* Please select Quantity before adding items.

6-Aminofluorescein 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

6-Aminofluorescein (6-AF) is a new fluorescence marker.

分子量

347.32

Formula

C20H13NO5

CAS 号

51649-83-3

中文名称

6-氨基荧光素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (287.92 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.8792 mL 14.3959 mL 28.7919 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL
10 mM 0.2879 mL 1.4396 mL 2.8792 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.99 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.99 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。

荧光染料6-Aminofluorescein(Synonyms: 6-氨基荧光素 6-AF)

荧光染料Dye Reagents 6-Aminofluorescein;(Synonyms: 6-氨基荧光素; 6-AF) 纯度: 98.63%

6-Aminofluorescein (6-AF) 是一种新型荧光标记物。

6-Aminofluorescein(Synonyms: 6-氨基荧光素; 6-AF)

6-Aminofluorescein Chemical Structure

CAS No. : 51649-83-3

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥770 In-stock
100 mg ¥700 In-stock
500 mg ¥1600 In-stock
1 g ¥2600 In-stock
5 g ; 询价 ;
10 g ; 询价 ;

* Please select Quantity before adding items.

6-Aminofluorescein 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

6-Aminofluorescein (6-AF) is a new fluorescence marker.

分子量

347.32

Formula

C20H13NO5

CAS 号

51649-83-3

中文名称

6-氨基荧光素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (287.92 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.8792 mL 14.3959 mL 28.7919 mL
5 mM 0.5758 mL 2.8792 mL 5.7584 mL
10 mM 0.2879 mL 1.4396 mL 2.8792 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.99 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.99 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.99 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。

荧光染料CLR1501

荧光染料Dye Reagents CLR1501;

CLR1501 是荧光标记的、CLR1404 的类似物,是具有癌细胞选择性的细胞荧光标记物。

CLR1501

CLR1501 Chemical Structure

CAS No. : 1250963-36-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

CLR1501, a fluorescently labeled CLR1404 analog, is a cancer cell-selective fluorescence compound[1][2].

体外研究
(In Vitro)

CLR1501 exhibits five- to ninefold preferential uptake in these cancer cell lines in vitro compared to normal fibroblasts[1].
Excitation/emission peaks are 500/517 nm for CLR1501. CLR1501 exhibits tumor selectivity in vitro and in vivo for human glioblastoma stem-like (GSC) cell lines and xenografts[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

701.67

Formula

C38H59BF2N3O4P

CAS 号

1250963-36-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Weichert JP, et al. Alkylphosphocholine analogs for broad-spectrum cancer imaging and therapy. Sci Transl Med. 2014 Jun 11;6(240):240ra75.

    [2]. Swanson KI, et al. Fluorescent cancer-selective alkylphosphocholine analogs for intraoperative glioma detection. Neurosurgery. 2015 Feb;76(2):115-23; discussion 123-4.

荧光染料DiSC3(5)

荧光染料Dye Reagents DiSC3(5);

DiSC3(5) 是一种荧光探针,常用作示踪染料来评估线粒体膜电位。

DiSC3(5)

DiSC3(5) Chemical Structure

CAS No. : 53213-94-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

DiSC3(5) is a fluorescent probe often used as a tracer dye to assess the mitochondrial membrane potential[1].

分子量

546.53

Formula

C25H27IN2S2

CAS 号

53213-94-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Yamamoto T, et al. Multiple effects of DiS-C3(5) on mitochondrial structure and function. Eur J Biochem. 2004;271(17):3573-3579.

荧光染料Zinquin

荧光染料Dye Reagents Zinquin; 纯度: ge;99.0%

Zinquin 是一种荧光传感器,可用于观察活性锌离子。λexem = 364/385 nm。

Zinquinamp;;

Zinquin Chemical Structure

CAS No. : 151606-29-0

规格 价格 是否有货
5 mg ¥3800 询问价格 货期

* Please select Quantity before adding items.

Zinquin 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Zinquin is a fluorescent sensor and used to observe reactive Zn2+. λexem = 364/385 nm.

分子量

386.42

Formula

C19H18N2O5S

CAS 号

151606-29-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 50 mg/mL (129.39 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.5879 mL 12.9393 mL 25.8786 mL
5 mM 0.5176 mL 2.5879 mL 5.1757 mL
10 mM 0.2588 mL 1.2939 mL 2.5879 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Nowakowski A, et al. Sensor specific imaging of proteomic Zn2+ with zinquin and TSQ after cellular exposure to N-ethylmaleimide. Metallomics. 2012 May;4(5):448-56.