EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET)[1].
体外研究 (In Vitro)
The peptide sequence of EDANS is derived from a natural processing site for HIV-1 PR[1].Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that was linearly related to the extent of substrate hydrolysis[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
266.32
Formula
C12H14N2O3S
CAS 号
50402-56-7
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, protect from light, stored under nitrogen
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;
DMSO : 13.89 mg/mL (52.16 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
3.7549 mL
18.7744 mL
37.5488 mL
5 mM
0.7510 mL
3.7549 mL
7.5098 mL
10 mM
0.3755 mL
1.8774 mL
3.7549 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
[1]. E D Matayoshi, et al. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. Science. 1990 Feb 23;247(4945):954-8.
DiI (DiIC18(3)) is a lipophilic carbocyanine fluorescent dye for membrane labeling. DiI has an absorption maximum at 549 nm and an emission maximum 565 nm. DiI is mildly fluorescent in aqueous suspension, but becomes bright when bound to cell membrane.
体外研究 (In Vitro)
Once bound to a membrane it diffuses laterally in 2 dimensions; if there is no diffusion barrier, DiI generally stains the whole leaflet (one surface) of a biological membrane rapidly, but does not readily flip across to the other leaflet.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
DiI-labeled motoneurons have remained viable for up to 4 weeks in culture and up to one year in vivo[1]
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
933.87
Formula
C59H97ClN2O4
CAS 号
41085-99-8
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 12.5 mg/mL (13.39 mM; ultrasonic and warming and heat to 80°C)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.0708 mL
5.3541 mL
10.7081 mL
5 mM
0.2142 mL
1.0708 mL
2.1416 mL
10 mM
0.1071 mL
0.5354 mL
1.0708 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. D P Kuffler, et al. Long-term survival and sprouting in culture by motoneurons isolated from the spinal cord of adult frogs. J Comp Neurol. 1990 Dec 22;302(4):729-38.
EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET)[1].
体外研究 (In Vitro)
The peptide sequence of EDANS is derived from a natural processing site for HIV-1 PR[1].Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that was linearly related to the extent of substrate hydrolysis[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
266.32
Formula
C12H14N2O3S
CAS 号
50402-56-7
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, protect from light, stored under nitrogen
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;
DMSO : 13.89 mg/mL (52.16 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
3.7549 mL
18.7744 mL
37.5488 mL
5 mM
0.7510 mL
3.7549 mL
7.5098 mL
10 mM
0.3755 mL
1.8774 mL
3.7549 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
[1]. E D Matayoshi, et al. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. Science. 1990 Feb 23;247(4945):954-8.
HBC525 is a HBC-like fluorophore and a fluorogenic RNA aptamer (Kd=3.8 nM). HBC525 can be directly used as fusion tags for the imaging and tracking of cellular RNAs of interest. Fluorogenic RNA aptamers have also been used to construct various interesting dynamic RNA nanodevices for cellular target detection and imaging[1][2].
IC50 Target
HBC525: 491/525 nm (Ex/Em)[2]
体内研究 (In Vivo)
荧光RNA适体
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
319.36
Formula
C19H17N3O2
CAS 号
2174014-64-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Chen X, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293.
[2]. Yu Q, et al. Genetically encoded RNA nanodevices for cellular imaging and regulation. Nanoscale. 2021;13(17):7988-8003.
Fluorescein diacetate 是一种细胞渗透性的酯酶底物。Fluorescein diacetate 可作为人谷胱甘肽硫转移酶 Pi (hGSTP1-1) 的荧光底物。
Fluorescein Diacetate Chemical Structure
CAS No. : 596-09-8
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10;mM;*;1 mL in DMSO
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Fluorescein Diacetate 相关产品
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Bioactive Compound Library Plus
生物活性
Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.
体外研究 (In Vitro)
Fluorescein diacetate (FDA) is an acetylated derivative of the green fluorescent dye fluorescein[1]. Fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human Pi-class glutathione S-transferase (hGSTP1) selectively among various cytosolic GSTs. Fluorescence activation of Fluorescein diacetate susceptible to GST inhibitors is observed in MCF7 cells exogenously overexpressing hGSTP1, but not in cells overexpressing hGSTA1 or hGSTM1. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. To investigate whether the fluorescence activation is due to hGSTP1 activity, Fluorescein diacetate is incubated with recombinant hGSTP1-1 and GSH in vitro. Remarkable fluorescence activation is observed in the presence of both hGSTP1-1 and GSH, whereas only slight activation is observed in the absence of either of them or when the enzyme is heat inactivated. This suggests that the fluorescence activation of Fluorescein diacetate depends on hGSTP1-1 activity. From the linear relationship between the rate of increase in fluorescence and the hGSTP1-1 concentration, the specific activity of the enzyme for 1 μM Fluorescein diacetate is determined to be 79±15 nmol/min/mg protein. Fluorescein diacetate is applicable as a fluorogenic substrate for evaluating inhibitors of GSTP1-1 in vitro. For Fluorescein diacetate as a substrate, both Ethacrynic acid (EA) and NBDHEX suppress the hGSTP1-1-dependent fluorescent increase in a concentration-dependent manner, with IC50s of 3.3±0.5 μM and 0.61±0.04 μM, respectively[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
416.38
Formula
C24H16O7
CAS 号
596-09-8
中文名称
二乙酸荧光素;荧光素二乙酸盐;荧光素二乙酸酯
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84.
[2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.
Cell Assay [2]
MCF7 cells (2-3×105) are seeded in a 35-mm glass bottom dish before the experiment. Prior to imaging, cells are washed with 1 mL PBS, then incubated in 1 mL Hanks’ Balanced Salt solution (HBSS(+) without phenol red) containing 1 μM Fluorescein diacetate (0.1% DMSO as a cosolvent) for 5 min at 37°C. Cells are washed twice with 1 mL PBS and 1 mL HBSS is added before imaging. Fluorescence images of fluorescein and DsRed-Express2 were acquired in the FITC channel (excitation at 473 nm) and the DsRed channel (excitation 559 nm). The 16-bit images obtained are analysed[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84.
[2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.
CLR1501, a fluorescently labeled CLR1404 analog, is a cancer cell-selective fluorescence compound[1][2].
体外研究 (In Vitro)
CLR1501 exhibits five- to ninefold preferential uptake in these cancer cell lines in vitro compared to normal fibroblasts[1]. Excitation/emission peaks are 500/517 nm for CLR1501. CLR1501 exhibits tumor selectivity in vitro and in vivo for human glioblastoma stem-like (GSC) cell lines and xenografts[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
701.67
Formula
C38H59BF2N3O4P
CAS 号
1250963-36-0
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Weichert JP, et al. Alkylphosphocholine analogs for broad-spectrum cancer imaging and therapy. Sci Transl Med. 2014 Jun 11;6(240):240ra75.
[2]. Swanson KI, et al. Fluorescent cancer-selective alkylphosphocholine analogs for intraoperative glioma detection. Neurosurgery. 2015 Feb;76(2):115-23; discussion 123-4.
[1]. Nowakowski A, et al. Sensor specific imaging of proteomic Zn2+ with zinquin and TSQ after cellular exposure to N-ethylmaleimide. Metallomics. 2012 May;4(5):448-56.