Tetrazolium Red(2,3,5-Triphenyltetrazolium chloride; TPTZ) is used to visualize dehydrogenase enzyme activity; initially the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.
分子量
334.80
Formula
C19H15ClN4
CAS 号
298-96-4
中文名称
红四氮唑;四氮唑红;红四唑;四唑红
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
H2O : 50 mg/mL (149.34 mM; Need ultrasonic)
DMSO : 16.67 mg/mL (49.79 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
2.9869 mL
14.9343 mL
29.8686 mL
5 mM
0.5974 mL
2.9869 mL
5.9737 mL
10 mM
0.2987 mL
1.4934 mL
2.9869 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
Nitro blue tetrazolium chloride;(Synonyms: NBT) 纯度: 99.82%
Nitro blue tetrazolium chloride (NBT) 是脱氢酶底物,与碱性磷酸酶底物BCIP一起用于western blotting和免疫组化染色。
Nitro blue tetrazolium chloride Chemical Structure
CAS No. : 298-83-9
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数量
Free Sample (0.1-0.5 mg)
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10;mM;*;1 mL in DMSO
¥550
In-stock
250 mg
¥500
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500 mg
¥800
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1 g
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5 g
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Nitro blue tetrazolium chloride 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.
分子量
817.64
Formula
C40H30Cl2N10O6
CAS 号
298-83-9
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
70%DMF : 100 mg/mL (122.30 mM; Need ultrasonic)
DMSO : 33.33 mg/mL (40.76 mM; Need ultrasonic)
H2O : 8 mg/mL (9.78 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.2230 mL
6.1152 mL
12.2303 mL
5 mM
0.2446 mL
1.2230 mL
2.4461 mL
10 mM
0.1223 mL
0.6115 mL
1.2230 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: