TMRE;(Synonyms: Tetramethylrhodamine ethyl ester perchlorate) 纯度: 98.24%
TMRE 是一种线粒体特异性染料 (λex=550 nm,λem=575 nm)。
TMRE Chemical Structure
CAS No. : 115532-52-0
规格 | 价格 | 是否有货 | 数量 |
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10;mM;*;1 mL in DMSO | ¥680 | In-stock | |
5 mg | ¥600 | In-stock | |
10 mg | ¥900 | In-stock | |
25 mg | ¥1800 | In-stock | |
50 mg | ; | 询价 | ; |
100 mg | ; | 询价 | ; |
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TMRE 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
生物活性 |
TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm). |
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体外研究 (In Vitro) |
TMRE is a mitochondria specific dye (λex=550 nm, λem=575 nm)[1]. Multidirectional dynamic movement of TMRE is observed in epithelial cells and bidirectional dynamic movement is seen in the superficial cortical fiber cells of live bovine lenses. In the epithelium, the movement of TMRE fluorescence is up to 5 μm/min whereas in the superficial cortex the observed movement is up to 18.5 μm/min. The movement of TMRE fluorescence is abolished with treatment of the uncoupler, carbonyl cyanide m-chlorophenylhydrazone (CCCP)[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
514.95 |
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Formula |
C26H27ClN2O7 |
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CAS 号 |
115532-52-0 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
4deg;C, sealed storage, away from moisture and light *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light) |
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溶解性数据 |
In Vitro:;
DMSO : 27.78 mg/mL (53.95 mM; Need ultrasonic) 配制储备液
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
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参考文献 |
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Cell Assay [1] |
The entire experiment should be performed at room temperature because temperature will directly impact mitochondrial transmembrane potential and TMRE staining. Cells should never be placed, centrifuged, incubated, or washed at 4°C or have ice-cold buffers or media added. Treat the cells with a cytotoxic stimulus. Harvest cells and resuspend at 5×105 cells/mL in culture medium containing 150 nM TMRE. Incubate for 5 min at room temperature in the dark. Add Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (5 μM final concentration) to an aliquot of untreated cells and incubate for 5 min at room temperature in the dark. Turn on the appropriate laser on the flow cytometer. Set up a histogram plot to detect TMRE using log scale[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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