Ganoderic acid DM, a natural triterpenoid isolated from Ganoderma lucidum, induces DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells. Ganoderic acid DM as a specific inhibitor of osteoclastogenesis[1][2].
体外研究 (In Vitro)
Ganoderic acid DM (GADM) effectively inhibits cell proliferation and colony formation in MCF-7 human breast cancer cells, which was much stronger than that of MDA-MB-231 breast cancer cells[1]. Ganoderic acid DM especially suppresses the expression of c-Fos and nuclear factor of activated T cells c1 (NFATc1). Ganoderic acid DM markedly suppressed the expression of cathepsin K and TRAP mRNA[2]. Ganoderic acid DM induces autophagic apoptosis in non-small cell lung cancer cells by inhibiting the PI3K/Akt/mTOR activity[3].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
Cell Line:
MCF-7 and MDA-MB-231 cells.
Concentration:
0-100 μM.
Incubation Time:
48 h.
Result:
Decreased the cell viability in breast cancer cells.
Cell Viability Assay[2]
Cell Line:
RAW-D cells.
Concentration:
0-100 μg/mL.
Incubation Time:
0-100 μg/mL.
Result:
Clearly suppressed osteoclastogenesis from the RAW 264 cell D-clone.
分子量
468.67
Formula
C30H44O4
CAS 号
173075-45-1
中文名称
灵芝酸 DM
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Guo-Sheng Wu, et al. Ganoderic acid DM, a natural triterpenoid, induces DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells. Fitoterapia. 2012 Mar;83(2):408-14.
[2]. Ichiko Miyamoto, et al. Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum. Eur J Pharmacol. 2009 Jan 5;602(1):1-7.
[3]. Junbo Xia, et al. Ganoderic acid DM induces autophagic apoptosis in non-small cell lung cancer cells by inhibiting the PI3K/Akt/mTOR activity. Chem Biol Interact. 2020 Jan 25;316:108932.
Quillaic acid(Quillaja sapogenin) is the major aglycone of the widely studied saponins of the Chilean indigenous tree Quillaja saponaria Mol; can elicit dose-dependent antinociceptive effects in two murine thermal models.
分子量
486.68
Formula
C30H46O5
CAS 号
631-01-6
中文名称
皂皮酸
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Arrau S, et al. Antinociceptive activity of Quillaja saponaria Mol. saponin extract, quillaic acid and derivatives in mice. J Ethnopharmacol. 2011 Jan 7;133(1):164-7.
[2]. Rodríguez-Díaz M, et al. Topical anti-inflammatory activity of quillaic acid from Quillaja saponaria Mol. and some derivatives. J Pharm Pharmacol. 2011 May;63(5):718-24.
上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。
Englerin A
Englerin A 是一种有效和选择性的 TRPC4 和 TRPC5 通道的活化剂,EC50 值分别为 11.2 和 7.6 nM。Englerin A 通过增加 Ca2+ 内流和 Ca2+ 细胞超负荷来诱导肾癌细胞死亡。
Englerin A Chemical Structure
CAS No. : 1094250-15-3
规格
是否有货
5 mg
询价
10 mg
询价
25 mg
询价
* Please select Quantity before adding items.
生物活性
Englerin A is a potent and selective activator of TRPC4 and TRPC5 channels, with EC50s of 11.2 and 7.6 nM, respectively. Englerin A can induce renal carcinoma cells death by elevated Ca2+ influx and Ca2+ cell overload[1][2][3].
IC50 & Target[1]
hTRPC4
11.2 nM (EC50)
hTRPC5
7.6 nM (EC50)
体外研究 (In Vitro)
Englerin A (0.15-2500 nM; 48 h) characteristic concentration-dependent suppresses cells growth of the A-498 cells treated with the control siRNA while has little effect on the growth of cells treated with TRPC4 siRNAs[3]. Englerin A (0.001nM-10 μM; 48 h) inhibits cells viability in HEK293T cells overexpressing TRPC5[3]. Englerin A (3 nM; 1-240 s) evokes sustained elevation of the intracellular Ca2+ concentration within 1 min in HEK 293 cells over-expressing human TRPC4[1]. Englerin A (0.1-1000 nM; 1-300 s) evokes intracellular Ca2+ elevations in A498 cells as well with an EC50 of 10 nM[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
Englerin A (5 mg/kg; i.p daily except Sunday) markedly inhibits tumor growth during the 2-week treatment period in mice[4].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
442.54
Formula
C26H34O6
CAS 号
1094250-15-3
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Akbulut Y, et, al. (-)-Englerin A is a potent and selective activator of TRPC4 and TRPC5 calcium channels. Angew Chem Int Ed Engl. 2015 Mar 16;54(12):3787-91.
[2]. Rubaiy HN, et, al. Identification of an (-)-englerin A analogue, which antagonizes (-)-englerin A at TRPC1/4/5 channels. Br J Pharmacol. 2018 Mar; 175(5):830-839.
[3]. Carson C, et, al. Englerin A Agonizes the TRPC4/C5 Cation Channels to Inhibit Tumor Cell Line Proliferation. PLoS One. 2015 Jun 22;10(6):e0127498.
[4]. Sourbier C, et, al. Englerin A stimulates PKCθ to inhibit insulin signaling and to simultaneously activate HSF1: pharmacologically induced synthetic lethality. Cancer Cell. 2013 Feb 11;23(2):228-37.
[1]. Matsuzawa Y, et al. Activation of cytosolic phospholipase A2alpha by epidermal growth factor (EGF) and phorbol ester in HeLa cells: different effects of inhibitors for EGF receptor, protein kinase C, Src, and C-Raf. J Pharmacol Sci. 2009 Oct;111(2):182-92
[1]. Sakurai N, et al. Cancer preventive agents. Part 1: chemopreventive potential of cimigenol, cimigenol-3,15-dione, and related compounds. Bioorg Med Chem. 2005 Feb 15;13(4):1403-8.
上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。
Eucalyptol(Synonyms: 1,8-Cineole) 纯度: ≥98.0%
Eucalyptol 是 5-HT3 受体, 钾通道, TNF-α 和 IL-1β 的抑制剂。
Eucalyptol Chemical Structure
CAS No. : 470-82-6
规格
价格
是否有货
数量
50 mg
¥700
In-stock
100 mg
¥1050
询价
500 mg
¥2850
询价
1 g
询价
5 g
询价
* Please select Quantity before adding items.
生物活性
Eucalyptol is an inhibitor of 5-HT3 receptor ,potassium channel, TNF-α and IL-1β.
IC50 & Target
5-HT3 Receptor
IL-1β
TNF-α
potassium channel
体外研究 (In Vitro)
Eucalyptol inhibits 5-HT-evoked currents in oocytes expressing 5-HT3 receptors with an IC50 of 258 µM[1]. Eucalyptol (Cin) treatment significantly decreases the ROS level in Aβ25-35 treated cells in a dose dependent manner. Eucalyptol treatment significantly decreases the NO level in Aβ25-35 treated cells in a dose dependent manner (p<0.05 and p<0.01). Eucalyptol treatment also significantly decreases IL-1βlevel in Aβ25-35 treated cells in a dose dependent manner (p<0.05 and p<0.01) as compare to Aβ25-35 treated PC12 cells. IL-6 level is also attenuated by Eucalyptol in dose dependent manner (p<0.05 and p<0.01) as compare to Aβ25-35 treated cells[3].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
Results show that male and female rats treated with Eucalyptol (CIN) at the highest doses, 500 and 1000 mg/kg, have shown lower body weight than control group from the 7th to 50th day of treatment. The administration of Eucalyptol significantly reduces body weight gain of male rats (Eucalyptol 500 and 1000 mg/kg) and female rats (Eucalyptol 1000 mg/kg) in the first week of treatment. However, this reduction is followed by an increase in body weight of rats males and females treated with all doses of the second week until the end of treatment. For male rats, there is a significant increase of 6.93% in mean corpuscular volume (MCV) (Eucalyptol 1000 mg/kg) and of 43.54 and 38.98% in the platelet count (Eucalyptol 500 and 1000 mg/kg, respectively) and a decrease of 6.74 and 6.67% in mean corpuscular hemoglobin concentration (MCHC) (Eucalyptol 500 and 1000 mg/kg) and mean platelet volume (MPV) of 10.40, 10.60 and 15.73% (Eucalyptol 100, 500 and 1000 mg/kg, respectively), when compare to the control group[4].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
154.25
Formula
C10H18O
CAS 号
470-82-6
中文名称
桉油精;桉树脑
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Jarvis GE, et al. Noncompetitive Inhibition of 5-HT3 Receptors by Citral, Linalool, and Eucalyptol Revealed by Nonlinear Mixed-Effects Modeling. J Pharmacol Exp Ther. 2016 Mar;356(3):549-62.
[2]. Zeraatpisheh Z, et al. Eucalyptol induces hyperexcitability and epileptiform activity in snail neurons by inhibiting potassium channels. Eur J Pharmacol. 2015 Oct 5;764:70-8.
[3]. Khan A, et al. 1,8-cineole (eucalyptol) mitigates inflammation in amyloid Beta toxicated PC12 cells: relevance to Alzheimer’s disease. Neurochem Res. 2014 Feb;39(2):344-52.[3]
[4]. Caldas GF, et al. Repeated-doses and reproductive toxicity studies of the monoterpene 1,8-cineole (eucalyptol) in Wistar rats. Food Chem Toxicol. 2016 Nov;97:297-306.
Cell Assay [3]
The protective dose of Eucalyptol (Cineole) is determined by MTT dye-uptake method. In brief, cells (1×104 per well) are seeded in 96-well tissue culture plates and allowed to adhere for 24 h in CO2 incubator at 37°C. Cells are differentiated for the indicated time period. Thereafter, the medium is replaced with the medium containing Eucalyptol (0 to 10 μM) in different experiments for a period up to 24 h. Tetrazolium bromide salt (5 mg/mL of stock in PBS) 10 μL/well is added in 100 mL of cell suspension and plate is incubated for 4 h. At the end of incubation period, the reaction mixture is carefully taken out and 200 μL of DMSO is added to each well by pipetting up and down several times until the content gets homogenized. The plates are kept on rocker shaker for 10 min at room temperature and then read at 550 nm using microplate reader[3].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration [4]
Swiss mice are used in this experiment. The animals are randomly divided into two groups (n=5) and fasted overnight with free access to water. The group control receives a 1% Tween-80 aqueous solution (0.1 mL/10 g) and the other group is treated with Eucalyptol a single 2000 mg/kg dose by oral route. The animals are observed at 30, 60, 120, 180 and 240 min after oral treatment and daily for 14 days. Behavioral changes, weight, food and water consumption, clinical signs of toxicity or mortality are recorded daily[4].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Jarvis GE, et al. Noncompetitive Inhibition of 5-HT3 Receptors by Citral, Linalool, and Eucalyptol Revealed by Nonlinear Mixed-Effects Modeling. J Pharmacol Exp Ther. 2016 Mar;356(3):549-62.
[2]. Zeraatpisheh Z, et al. Eucalyptol induces hyperexcitability and epileptiform activity in snail neurons by inhibiting potassium channels. Eur J Pharmacol. 2015 Oct 5;764:70-8.
[3]. Khan A, et al. 1,8-cineole (eucalyptol) mitigates inflammation in amyloid Beta toxicated PC12 cells: relevance to Alzheimer’s disease. Neurochem Res. 2014 Feb;39(2):344-52.[3]
[4]. Caldas GF, et al. Repeated-doses and reproductive toxicity studies of the monoterpene 1,8-cineole (eucalyptol) in Wistar rats. Food Chem Toxicol. 2016 Nov;97:297-306.
上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。
Ganoderic acid Y(Synonyms: 灵芝酸Y) 纯度: 99.07%
Ganoderic acid Y 是一种 α-葡萄糖苷酶 (α-glucosidase) 抑制剂,对酵母 α-葡萄糖苷酶的 IC50 为170 μM。Ganoderic acid Y 通过阻止肠道病毒 71 (EV71) 的脱膜而抑制其复制。
Ganoderic acid Y Chemical Structure
CAS No. : 86377-52-8
规格
价格
是否有货
数量
1 mg
¥6000
In-stock
5 mg
询价
10 mg
询价
* Please select Quantity before adding items.
生物活性
Ganoderic acid Y is a α-glucosidase inhibitor with an IC50 of 170 μM for yeast α-glucosidase. Ganoderic acid Y inhibits enterovirus 71 (EV71) replication through blocking EV71 uncoating[1][2].
体外研究 (In Vitro)
Ganoderic acid Y significantly inhibits the replication of the viral RNA (vRNA) of EV71[2].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
454.68
Formula
C30H46O3
CAS 号
86377-52-8
中文名称
灵芝酸Y
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Chen XQ, et al. Lanostane triterpenes from the mushroom Ganoderma resinaceum and their inhibitory activities against α-glucosidase. Phytochemistry. 2018 May;149:103-115.
[2]. Zhang W, et al. Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection. Biochem Biophys Res Commun. 2014 Jul 4;449(3):307-12.
7-O-Geranylscopoletin is a coumarin from the root of Atalantia monophylla. Various parts of this plant have been used for folk medicine for several purposes such as chronic rheumatism, paralysis, antispasmodic, stimulant and hemiplegia[1].
分子量
328.40
Formula
C20H24O4
CAS 号
28587-43-1
中文名称
7-香叶草氧基-6-甲氧基香豆素
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
参考文献
[1]. Chukaew A, et al. Potential anti-allergic acridone alkaloids from the roots of Atalantia monophylla. Phytochemistry. 2008 Oct;69(14):2616-20.
上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。
Platycoside E(Synonyms: 桔梗苷E) 纯度: ≥98.0%
Platycoside E 是从桔梗的根中分离出的桔梗皂苷元型皂苷,具有溶血活性和佐剂潜力。Platycoside E 促进 ovalbumin (OVA) 免疫小鼠中血清 OVA 特异性 IgG2a 和 IgG2b 抗体的产生。
Platycoside E Chemical Structure
CAS No. : 237068-41-6
规格
价格
是否有货
数量
1 mg
¥2860
In-stock
5 mg
¥8570
In-stock
10 mg
询价
50 mg
询价
* Please select Quantity before adding items.
Platycoside E 相关产品
•相关化合物库:
Natural Product Library Plus
Bioactive Compound Library Plus
生物活性
Platycoside E is a platycodigenin-type saponin isolated from the root of Platycodon grandiflorum with haemolytic activity and adjuvant potential. Platycoside E promotes the production of the sera OVA-specific IgG2a and IgG2b antibody in the ovalbumin (OVA)-immunized mice[1].
IC50 & Target
IC50: OVA-specific IgG2a and IgG2b antibody[1]
分子量
1549.60
Formula
C69H112O38
CAS 号
237068-41-6
中文名称
桔梗苷E
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Xie Y, et al. Contribution of the glycidic moieties to the haemolytic and adjuvant activity of platycodigenin-type saponins from the root of Platycodon grandiflorum.Vaccine. 2008 Jun 25;26(27-28):3452-60.