K114, a fluorescent Congo Red analogue, binds tightly to amyloid fibrils with an EC₅₀ of 20-30 nM^[1]. K114 is an efficient detector of semen-derived enhancer of virus infection (SEVI)^[2].

K114’s unusually low buffer fluorescence is due to self-quenching in sedimentable aggregates or micelles which upon interacting with amyloid fibrils undergo an enhancement in fluorescence intensity and shifts in the excitation and emission spectra^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

393.27

C₂₂H₁₇BrO₂

872201-12-2

Room temperature in continental US; may vary elsewhere.

DMSO : 100 mg/mL (254.28 mM; Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. LeVine H 3rd, et al. Mechanism of A beta(1-40) fibril-induced fluorescence of (trans,trans)-1-bromo-2,5-bis(4-hydroxystyryl)benzene (K114). Biochemistry. 2005 Dec 6;44(48):15937-43.

  [2]. Selmani V, et al. K114 (trans, trans)-bromo-2,5-bis(4-hydroxystyryl)benzene is an efficient detector of cationic amyloid fibrils. Protein Sci. 2015 Mar;24(3):420-5.