标签归档：acridine

Acridine Orange 10-Nonyl Bromide(Synonyms: 10-壬基溴代吖啶橙 Nonylacridine orange)

发表于由

Acridine Orange 10-Nonyl Bromide;(Synonyms: 10-壬基溴代吖啶橙; Nonylacridine orange) 纯度: 99.50%

Acridine Orange 10-Nonyl Bromide 是心磷脂的荧光探针 (λex: 489 nm, λem: 525 nm)。

Acridine Orange 10-Nonyl Bromideamp;;(Synonyms: 10-壬基溴代吖啶橙; Nonylacridine orange)

Acridine Orange 10-Nonyl Bromide Chemical Structure

CAS No. : 75168-11-5

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥990 In-stock
10 mg ￥900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Acridine Orange 10-Nonyl Bromide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm).

体内研究
(In Vivo)

Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria[1]. when Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

472.50

Formula

C26H38BrN3
CAS 号

75168-11-5
中文名称

10-壬基溴代吖啶橙；吖啶橙10-溴壬烷
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 33.33 mg/mL (70.54 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1164 mL 10.5820 mL 21.1640 mL
5 mM 0.4233 mL 2.1164 mL 4.2328 mL
10 mM 0.2116 mL 1.0582 mL 2.1164 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 1.67 mg/mL (3.53 mM); Clear solution

    此方案可获得 ≥ 1.67 mg/mL (3.53 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.67 mg/mL (3.53 mM); Clear solution

    此方案可获得 ≥ 1.67 mg/mL (3.53 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Ratinaud MH, et al. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry. 1988 May;9(3):206-12.

    [2]. Gallet PF, et al. Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange. Eur J Biochem. 1995 Feb 15;228(1):113-9.
Cell Assay
[2]

Yeast cells in log phase are fixed in cold ethanol (70% by vol.) and stored at -20°C. Fixed cells are washed three times with cold 10 mM Tris/HCI pH 7, then mildly sonicated to eliminate aggregates and finally counted. Yeast cells are added to 45 μM Acridine Orange 10-Nonyl Bromide and incubated for 15 min at 20°C. Cells are centrifuged (3000×g, 5 min) then washed twice in 10 mM Tris/HCl pH 7. Red fluorescence of Acridine Orange 10-Nonyl Bromide bound to 106 yeast cells is measured at 640 nm and correlated to the calibration curve run with thin-walled vesicles containing known amounts of cardiolipin[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Ratinaud MH, et al. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry. 1988 May;9(3):206-12.

    [2]. Gallet PF, et al. Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange. Eur J Biochem. 1995 Feb 15;228(1):113-9.

Acridine Orange hydrochloride hydrate 吖啶橙盐酸盐水合物 品牌：Chemodex

发表于由

Acridine Orange hydrochloride hydrate

吖啶橙盐酸盐水合物

品牌：Chemodex
CAS No.：65-61-2 | 1704465-79-1
储存条件：+4°C
纯度：≥98% (HPLC)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-A0005-G001

 – 1 g – 900.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Acridine Orange hydrochloride hydrate 吖啶橙盐酸盐水合物 品牌：Chemodex

发表于由

Acridine Orange hydrochloride hydrate

吖啶橙盐酸盐水合物

品牌：Chemodex
CAS No.：65-61-2 | 1704465-79-1
储存条件：+4°C
纯度：≥98% (HPLC)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-A0005-G005

 – 5 g – 3,560.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Acridine orange 10-nonyl bromide 10-壬基吖啶橙溴化物 品牌：Chemodex

发表于由

Acridine orange 10-nonyl bromide

10-壬基吖啶橙溴化物

品牌：Chemodex
CAS No.：75168-11-5
储存条件：+4°C
纯度：>90% (HPLC)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-A0103-M100

 – 100 mg – 1,960.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Acridine Orange hemi(zinc chloride) 吖啶橙 品牌：Chemodex

发表于由

Acridine Orange hemi(zinc chloride)

吖啶橙

品牌：Chemodex
CAS No.：10127-02-3
储存条件：+20°C
纯度：>90% (Dye content)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-A0668-G025

 – 25 g – 1,060.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Acridine Orange hemi(zinc chloride) 吖啶橙 品牌：Chemodex

发表于由

Acridine Orange hemi(zinc chloride)

吖啶橙

品牌：Chemodex
CAS No.：10127-02-3
储存条件：+20°C
纯度：>90% (Dye content)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-A0668-G100

 – 100 g – 3,460.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Acridine Orange hydrochloride(Synonyms: 吖啶橙)

发表于由

Acridine Orange hydrochloride;(Synonyms: 吖啶橙) 纯度: 99.72%

Acridine Orange hydrochloride 是与核酸结合的细胞渗透性荧光染料，导致光谱发射改变。

Acridine Orange hydrochlorideamp;;(Synonyms: 吖啶橙)

Acridine Orange hydrochloride Chemical Structure

CAS No. : 65-61-2

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥550 In-stock
100 mg ￥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Acridine Orange hydrochloride 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Blood Cancer Compound Library

生物活性

Acridine Orange hydrochloride is a cell-permeable fluorescent dye that binds to nucleic acids, resulting in an altered spectral emission.

体外研究
(In Vitro)

Acridine Orange has been employed extensively as a cytochemical stain and has shown to stain differentially DNA and RNA, and double-restranded nucleic acids in situ. Acridine Orange can either intercalate into double helical nucleic acids (green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (red fluorescence at 640 nm). This unique characteristic makes acridine orange useful for cell-cycle studies[1]. Acridine Orange staining of unfixed cells may be used as a simple, fast means of obtaining information on cell ploidy levels and cell cycle status from DNA measurements (green fluorescence), and cell transcriptional activity from RNA staining (red fluorescence), in human and murine cells lines, peripheral blood and bone marrow specimens from patients with leukemia and mitogenically (phytohemagglutinin) or antigenically (mixed lymphocyte culture) stimulated human peripheral blood cultures[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

301.81

Formula

C17H20ClN3
CAS 号

65-61-2
中文名称

吖啶橙；碱性橙 14；丫啶橙；3,6-双(二甲氨基)吖啶
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;

H2O : 50 mg/mL (165.67 mM; ultrasonic and heat to 60°C)

DMSO : 25 mg/mL (82.83 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3133 mL 16.5667 mL 33.1334 mL
5 mM 0.6627 mL 3.3133 mL 6.6267 mL
10 mM 0.3313 mL 1.6567 mL 3.3133 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 2.08 mg/mL (6.89 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (6.89 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.89 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.89 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.

    [2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.
Cell Assay

Two-step, pH 3.0: Aliquots (0.2 mL, containing approximately 2-5×105 cells) are withdrawn from cultures and are added to 0.5 mL of a solution containing: 0.1% (v/v) Triton X-100, 0.2 M sucrose, 10-4 M EDTA and 2×10-2 M citrate-phosphate buffer, at pH 3.0. Triton X-100 is included in the various procedures at the indicated pH to increase cell permeability yet maintain cellular integrity. The chelating agent EDTA is used to facilitate RNA denaturation. The cells are stained one minute later by addition of 1 mL of a solution containing 0.002% (20 μg/mL) AO, 0.1 M NaCl and 10-2 M citrate-phosphate buffer, pH 3.8. Cations are included in the staining mixture to ensure staining specificity. The final AO concentration is approximately 4×10-5 M[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.

    [2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.